Monthly Archives: October 2015

Reagents and Kits

Transfection reagents EndoFectin™ Max       EndoFectin™ Expi293       EndoFectin™ HepG2     Luciferase assays Secrete-Pair™ Dual Luminescence and Gaussia Luciferase Assay Kits       Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (Firefly and Renilla)       Secrete-Pair™ Gaussia Luciferase Assay Kits     EZShuttle™ cloning......
Posted in Products

Lentiviral & AAV Delivery Systems

Recombinant viral vectors are widely used gene delivery tools for cells, animal models, and clinical applicatons, due to their propensity to infect most cells and tissues, and for enhanced safety. GeneCopoeia’s comprehensive suite of viral solutions for lentivirus and adeno-associated virus (AAV) includes ready-to-use particles suitable for use in cell culture and animals, plasmid clones expressing ORF, promoter, miRNA, shRNA, and sgRNA, and packaging reagents for do-it-yourself lentiviral particle production.         ......
Posted in Viral systems

Clone collections

-Sequence-verified expression clones for ORF, sgRNA, shRNA, & more     ORF Clones Expression-ready ORF clones EZShuttle™ Gateway® PLUS ORF clones Safe Harbor Knock-in ORF clones for human and mouse   ORF clone sets– Save up to 90% per clone     CRISPR and TALEN clones GeneHero™ Cas9 and sgRNA clones  GeneHero™ CRISPR sgRNA libraries Safe Harbor Knock-in ORF clones for human and mouse Genome-TA......
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GLuc-ON™ Transcriptional Response Assays

Introduction TRE non-viral clones TRE lentiviral clones Luciferase assay kits Introduction GeneCopoeia's GLuc-ON™ transcriptional response element (TRE) clones enable rapid and sensitive signal pathway analysis in mammalian cell lines. Using a secreted Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ TRE  clones are designed for robust and sensitive activation of specific signaling pathways in response to environmental stimuli or other experimental manipulation. Each GLuc-ON™ TRE clone comes either in transfection-ready or len......
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ERSE transcriptional response element (TRE) clone

GeneCopoeia provides the GLuc-ON™ ERSE transcriptional response element (TRE) clone for analysis of the response to endoplasmic reticulum (ER) stress resulting from the unfolded protein response. The ER stress response element (ERSE) is activated by several transcription factors, namely ATF6, to induce genes that express proteins that clear the cell of misfolded and unfolded proteins. In cases of prolonged ER stress, the pathway induces apoptosis. Malfunction of the ER stress pathway leads to diseases such as Huntingtin's disease and diabetes. The GLuc-ON™ MEF2 ERSE clon......
Posted in Luciferase Assays and Reporters, Products

JNK transcriptional response element (TRE) clone

GeneCopoeia's GLuc-ON™ JNK transcriptional response element (TRE) clone enables analysis of the effects on genes regulated by activator protein 1 (AP1). AP1 is a transcription factor complex comprised of the c-Fos and c-Jun family proteins,  and is necessary for signaling through the Stress-activated protein kinases (SAPK)/Jun amino-terminal kinases (JNK) (SAPK/JNK) pathway. The GLuc-ON™ JNK TRE clone contains tandem repeats of an AP1 binding site upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis......
Posted in Luciferase Assays and Reporters, Products

MEF2 transcriptional response element (TRE) clone

GeneCopoeia's GLuc-ON™ MEF2 transcriptional response element (TRE) clone enables analysis of pathways that act through the myocyte enhancer factor 2 (MEF2) proteins. MEF2 is a family of 4 transcription factors (MEF2A, MEF2B, MEF2C, and MEF2D) in mammals that regulate genes involved in muscle development and cardiac stress. The GLuc-ON™ MEF2 TRE clone contains tandem repeats of an MEF2 binding site upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The MEF2 response elements ar......
Posted in Luciferase Assays and Reporters, Products

TCF/LEF transcriptional response element (TRE) clone

GeneCopoeia provides the GLuc-ON™ TCF/LEF transcriptional response element (TRE) clone for analysis of the effects on Wnt signaling pathways mediated by TCF/LEF transcription factors, which recruit β-catenin to activate the promoters of several genes critical for function of the Wnt pathway. Wnt signaling plays fundamental roles in embryonic development, and its misregulation has been implicated in diseases such as cancer and type 2 diabetes. The GLuc-ON™ TCEF/LEF TRE clone contains tandem repeats of an TCF/LEF binding site upstream of a minimal promoter and secreted Ga......
Posted in Luciferase Assays and Reporters, Products

EGR1 transcriptional response element (TRE) clone

GeneCopoeia's GLuc-ON™ EGR1 transcriptional response element (TRE) clone enables analysis of the effects on genes regulated by early growth respionse protein 1 (EGR1), such as  transforming growth factor-beta-1 (TGFB1) and fibronectin (FN1). EGR1 is a transcription factor that responds to mitogenic stimulation. EGR1 regulates many processes, such as neuron activity, wound healing, apopotosis, and angiogenesis, and is known to both suppress and promote tumor growth. The GLuc-ON™ EGR1 TRE clone contains tandem repeats of an EGR1 binding site upstream of a minimal prom......
Posted in Luciferase Assays and Reporters, Products

PKC transcriptional response element (TRE) clone

GeneCopoeia's GLuc-ON™ PKC transcriptional response element (TRE) clone enables analysis of the effects on genes regulated by activator protein 1 (AP1). AP1 is a transcription factor complex comprised of the c-Fos and c-Jun family proteins,  and is necessary for signaling through the Protein kinase C (PKC) pathway. The GLuc-ON™ PKC TRE clone contains tandem repeats of an AP1 binding site upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The AP1 response elements ar......
Posted in Luciferase Assays and Reporters, Products