by admin | May 28, 2012 | Products
For studying the role of miRNA or trying to validate a putative target, GeneCopoeia has the right solutions. GeneCopoeia miRNA tools will help you design effective experiments to answer your questions.
- What is the regulatory function of a specific miRNA? Do quantitative changes in its expression directly or indirectly lead to cancer or other diseases? If so, how much change?
- How can I quickly detect and measure miRNA expression levels?
- Which miRNA have an effect on my gene of interest?
- Does a particular miRNA have the same influence on the same target gene in a different cell population?
- Are there multiple targets for my miRNA? How can I validate a specific miRNA against a predicted target?
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GeneCopoeia provides solutions for every step of miRNA functional analysis and detection
Analyze target protein levels and study miRNA-target mRNA interaction
miTarget™ miRNA 3' UTR Target Sequence Expression Clones
Do functional validation of predicted miRNA targets using miRNA 3' UTR target clones in a single or Duo-Luciferase reporter vector system.
- The 3' UTR target constructs can be used to analyze and validate the effect of endogenous miRNA on target gene expression levels.
- Up or down regulation of miRNA can be studied by transfecting precursor or inhibitor miRNA with miRNA 3' UTR target constructs using the GeneCopoeia luciferase reporter system.
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LucPair™ miR Luciferase Assay Kit
To measure the interaction between miRNA-target mRNA levels, the LucPair™ miR Duo-Luciferase reporter assay kit has been optimized and developed for use with GeneCopoeia miRNA constructs.
Secrete-Pair™ Dual Luminescence Assay Kit
Live cell analysis for miRNA target clones by detecting secreted Gaussia luciferase and SEAP.
OmicsLink™ ORF cDNA Expression Clones
Target gene restoration studies can also be performed using GeneCopoeia ORF cDNA expression clones available in 100 expression vectors with a choice of more than 50 fusion tags.
Questions? Contact us here or email directly at inquiry@genecopoeia.com.
by admin | May 28, 2012 | Products
For studying the role of miRNA or trying to validate a putative target, GeneCopoeia has the right solutions. GeneCopoeia miRNA tools will help you design effective experiments to answer your questions.
- What is the regulatory function of a specific miRNA? Do quantitative changes in its expression directly or indirectly lead to cancer or other diseases? If so, how much change?
- How can I quickly detect and measure miRNA expression levels?
- Which miRNA have an effect on my gene of interest?
- Does a particular miRNA have the same influence on the same target gene in a different cell population?
- Are there multiple targets for my miRNA? How can I validate a specific miRNA against a predicted target?
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GeneCopoeia provides solutions for every step of miRNA functional analysis and detection
Analyze and detect the effect of miRNA on target mRNA levels
All-in-One™ cDNA Synthesis and qPCR Kits
To study the effect of miRNA on target mRNA expression levels, the All-in-One first-strand cDNA synthesis kit delivers robust cDNA from virtually any source of total or polyA RNA source.
The All-in-One qPCR mix and validated qPCR primers provide sensitive and specific data in real time.
Using high-fidelity hot-start polymerase, an optimized reaction buffer and quality dNTPs deliver universal reaction conditions.
All-in-One™ qPCR validated primers
All-in-One qPCR human- mouse- and rat-specific primers are designed by a proprietary algorithm and validated for precision performance.
- Uniform reaction conditions
- High amplification efficiency and sensitivity
- Absence of non-specific primers and primer-dimers
- Affordable
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Use of the three products (cDNA synthesis, qPCR and primers) which were co-developed results in one of the most reliable, affordable and robust systems available to generate fast miRNA quantitative assays.
Questions? Contact us here or email directly at inquiry@genecopoeia.com.
by admin | May 28, 2012 | Products
For studying the role of miRNA or trying to validate a putative target, GeneCopoeia has the right solutions. GeneCopoeia miRNA tools will help you design effective experiments to answer your questions.
- What is the regulatory function of a specific miRNA? Do quantitative changes in its expression directly or indirectly lead to cancer or other diseases? If so, how much change?
- How can I quickly detect and measure miRNA expression levels?
- Which miRNA have an effect on my gene of interest?
- Does a particular miRNA have the same influence on the same target gene in a different cell population?
- Are there multiple targets for my miRNA? How can I validate a specific miRNA against a predicted target?
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GeneCopoeia provides solutions for every step of miRNA functional analysis and detection
Transfection of target cells with miRNAs and generation of lentiviral particles
EndoFectin™ Transfection Reagents
A variety of optimized transfection reagents have been developed by GeneCopoeia resulting in efficient and easy transfection. Choose one to meet your needs:
- For transfection of non-viral precursor and inhibitor miRNA plasmid constructs into most classical cell lines, EndoFectin PLUS is the efficient and cost effective choice.
- For transfection of lentiviral vector-based precursor and inhibitor miRNA clones use EndoFectin Lenti reagent for both efficient transfection and high titers for particles.
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Lenti-Pac™ Lentiviral Packaging kits
Lentiviral packaging kits have been optimized for use with GeneCopoeia lentiviral constructs to achieve high titers and robust expression levels.
- Both HIV-and FIV-based lentiviral packaging kits are available and include EndoFectin Lenti and TiterBoost reagents for 10-fold higher titers.
- The components of the kit together with miRNA plasmids can be co-transfected into GeneCopoeia 293Ta cells to produce lentiviral particles for further transduction into non-dividing or difficult-to-transfect target cells.
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Lentifect™ 293Ta cells
For easy transfection and high level expression of viral proteins, GeneCopoeia offers 293Ta cells for co-transfecting miRNA constructs with packaging plasmids.
Questions? Contact us here or email directly at inquiry@genecopoeia.com.
by admin | May 25, 2012 | Products
For studying the role of miRNA or trying to validate a putative target, GeneCopoeia has the right solutions. GeneCopoeia miRNA tools will help you design effective experiments to answer your questions.
- What is the regulatory function of a specific miRNA? Do quantitative changes in its expression directly or indirectly lead to cancer or other diseases? If so, how much change?
- How can I quickly detect and measure miRNA expression levels?
- Which miRNA have an effect on my gene of interest?
- Does a particular miRNA have the same influence on the same target gene in a different cell population?
- Are there multiple targets for my miRNA? How can I validate a specific miRNA against a predicted target?
GeneCopoeia provides solutions for every step of miRNA functional analysis and detection
Overexpress or knockdown miRNA
miExpress™ precursor miRNA expression clones and miArrest™ miRNA inhibitors
Overexpression or suppression of miRNA can help identify target genes and/or study the effects of specific miRNA in cell culture models.
Once the endogenous miRNA expression level of a cell is determined, GeneCopoeia miRNA precursors or inhibitors can be used to over-express or knockdown miRNA levels and study gain-of-function or loss-of-function effects respectively.
The specific features and choices enable studies of all kinds of cells including difficult-to-transfect and non-dividing using miRNA constructs from GeneCopoeia.
- Transient or stable transfection
- Lentiviral or non-viral vector backbones
- Fluorescent reporter genes
- Choice of vector-based or synthetic oligonucleotide inhibitors
miRNA clone sets now available!
Questions? Contact us here or email directly at inquiry@genecopoeia.com.
by admin | May 15, 2012 | Uncategorized
