Monthly Archives: February 2014

qPCR arrays and reagents What can GeneCopoeia's miProfile™ miRNA qPCR arrays and ExProfile™ gene qPCR arrays do for you? Answer: With these arrays, you don't need to spend time on primer design and validation of genes and miRNAs. These arrays allow you to perform batch qPCR detection of genes or miRNAs simultaneously in the same 96-well or 384-well plate. This greatly simplifies and speeds up your experiments.   How do I order miProfile™ miRNA qPCR arrays and ExProfile™ gene qPCR arrays? Answer: GeneCopoeia offers custom services ......

Stable Cell Line Services What types of cell-based services do you offer? Answer: There are two types of related services we currently offer in various cell lines. One consists of the generation of mammalian stable cell lines for protein expression, gene knockdown, CRISPR- or TALEN-mediated genome modifications, and primary cell immortalization. The second type is cell-based validation, in which we offer validation of ORF and cDNA expression levels, shRNA knockdown efficiency, CRISPR sgRNA validation, interaction between miRNAs and their miRNA targets, and promoter activity. What......

Genome editing products General How do I order TALEN or CRISPR reagents from GeneCopoeia? Answer: If you are doing simple gene knockouts in humans, mice, or rats, you can order TALENs or CRISPR sgRNAs on our website. All you need to do is go to the TALEN/CRISPR search page, search for your gene, and then choose the appropriate clones that will work for your system. You can also order donor clones for these knockouts from the search results page. Please note that these TALENs and CRISPR sgRNAs are designed by default to target the protein coding-region as closely as possible to t......

shRNA clone sets How do I order shRNA clones from GeneCopoeia? Answer: All you need to do is go to the shRNA clones search page, search for your gene, and then choose the appropriate clones that will work for your system. If you have any custom requirements, then you will need to contact us and, after determining what you need, we will send you a custom quote. What is the delivery format of the shRNA clones Answer: GeneCopoeia provides a set containing 3 individual shRNA constructs and a scrambled control. Each clone is provided as of 5 µg purified plasmid. A datashe......

Lentivirus How do I order custom lentiviral particles from GeneCopoeia? Answer: The best way is to search for what you need on our lentiviral search page. You simply need to indicate the clone type (ORF, promoter, etc.), the gene information (gene symbol, aliases, description, nucleotide accession, Entrez gene ID, catalog or product ID), the vector type, and the delivery volume. You can also request a custom quote by contacting us. What volumes do you offer for your custom lentiviral particle preparations? Answer: 50 μl, 100 μl, 200 μl, and 400 μl, at titers of ......

ORF clones How do I order ORF clones from GeneCopoeia? Answer: All you need to do is go to the ORF clones search page, search for your gene, and then choose the appropriate clones that will work for your system. If you have any custom requirements, then you will need to contact us and, after determining what you need, we will send you a custom quote. What delivery formats do you offer for your ORF clones? Answer: We provide most ORF clones as 10 µg of purified plasmid. For our NextDay™ clones, you can choose between bacterial stock or transfection-ready DNA (10 ......