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miProfile™ Disease and Focus-Group miRNA qPCR Arrays

by | Oct 11, 2012 | Products

For focused group profiling of miRNA expression

  
*Promotions are valid in the US & Canada only. For international customers, please contact your local distributors. Discounts are not valid on previous purchases and cannot be combined with additional discounts.
Introduction
miProfile disease and focus-group miRNA qPCR arrays profile the expression of disease-related or other focus-group miRNAs, which are carefully chosen for their close disease, pathway, or process correlation based on a thorough literature search of peer-reviewed publications. miRNAs are important modulators in cellular pathways and are highly conserved in eukaryotic organisms. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases. The miProfile disease and focus-group arrays allow researchers to study the differential expression of these miRNAs to gain understanding of the role of miRNA in disease pathogenesis or cellular pathways as well as to identify or validate key markers.
To view other miProfile miRNA qPCR arrays, click below:

 

Key Advantages

Close correlation

  • miRNAs are carefully chosen for their close correlation to the specific diseases or research focus based on a thorough literature search of peer-reviewed publications

Robust performance

  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
  • Broad linearity– Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible – High reproducibility (R2>0.99) for inter-array and intra-array replicates

Validated miRNA primers

  • Each miRNA primer is designed using a proprietary algorithm and experimentally validated

miProfile™ Cancer miRNA qPCR Arrays

by | Oct 11, 2012 | Products

For focused group profiling of cancer-related miRNA expression

  
*Promotions are valid in the US & Canada only. For international customers, please contact your local distributors. Discounts are not valid on previous purchases and cannot be combined with additional discounts.
Introduction
miProfile cancer miRNA qPCR arrays profile the expression of cancer-related miRNAs, which are carefully chosen for their close cancer correlation based on a thorough literature search of peer-reviewed publications. Arrays are available for expression profiling of specific types of cancer-related miRNAs or overall cancer-related miRNAs.
miRNAs are known for their extensive involvement in cancer development and progression and their altered pattern of expression may affect the cell cycle and survival program. The miProfile cancer arrays allow researchers to profile the differential expression of cancer-related miRNAs in normal tissues and various cancers to gain understanding of the role of miRNA in cancer pathogenesis as well as to identify or validate markers for cancer classification, diagnosis, and prognosis.
To view other miProfile miRNA qPCR arrays, click below:

 

Key Advantages

Close cancer correlation

  • miRNAs are carefully chosen for their close cancer correlation based on a thorough literature search of peer-reviewed publications

Robust performance

  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
  • Broad linearity– Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible – High reproducibility (R2>0.99) for inter-array and intra-array replicates

Validated miRNA primers

  • Each miRNA primer is designed using a proprietary algorithm and experimentally validated

miProfile™ miRNome miRNA qPCR Arrays

by | Oct 9, 2012 | Products

For high-throughput profiling of miRNA whole genome expression
*Promotions are valid in the US & Canada only. For international customers, please contact your local distributors. Discounts are not valid on previous purchases and cannot be combined with additional discounts.
Introduction
The miProfile human miRNome miRNA qPCR arrays are a set of nineteen 96-well plates covering 1,700 of best characterized and annotated miRNAs based on miRBase V18. Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer) and 12 wells of different types of controls, which are used to monitor the efficiency of the entire experimental process – from reverse transcription to qPCR reaction.
Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. A cDNA pool, containing reverse transcript products of 10 different tissue total RNAs, was used as the qPCR validation template.
Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion. The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended  and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.
To help best distinguish single-nucleotide mismatched miRNAs, 61 single-nucleotide mismatched miRNAs from the human miRNome are further grouped together on one 96-well plate. The miProfile human single-nucleotide mismatch miRNA qPCR array is available as stand-alone products for users who want to study these miRNAs using specific PCR conditions.
To view other miProfile miRNA qPCR arrays, click below:
 

Key Advantages

Validated miRNA primers
  • Each miRNA primer is designed using a proprietary algorithm and experimentally validated
Robust performance
  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
  • Broad linearity– Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible – High reproducibility (R2>0.99) for inter-array and intra-array replicates
Largest coverage
  • More than 1,700 of best characterized and annotated miRNAs are covered based on miRBase V18

miProfile™ miRNA qPCR Arrays

by | Oct 8, 2012 | Products

Introduction

For high-throughput profiling of miRNA expression

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. Usually 21-23 nucleotides in length, miRNAs are important modulators in cellular pathways and are highly conserved in eukaryotic organisms. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases. The miProfile miRNA PCR Arrays are designed for profiling the expression of pre-defined or customized sets of miRNAs in various tissues or cells. The resulting differential expression of profiled miRNAs help researchers to identify those miRNAs that are of biological significance and importance relevant to their research. Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer) and 12 wells of different types of controls, which are used to monitor the efficiency of the entire experimental process – from reverse transcription to qPCR reaction. Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. A cDNA pool, containing reverse transcript products of 10 different tissue total RNAs, was used as the qPCR validation template. Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion.The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended  and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.

Key advantages

Validated miRNA primers
  • Each miRNA primer is designed using a proprietary algorithm and experimentally validated
Robust performance
  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
  • Broad linearity– Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible – High reproducibility (R2>0.99) for inter-array and intra-array replicates
Genome-wide coverage, pre-arranged groups, or customized groups
  • Largest genome-wide miRNA coverage
  • Cancer-related groups
  • Customized miRNA arrays for focused study
 
Figure 1. miRNA PCR array experimental work flow (left) and miRNA RT-PCR mechanism (right).
 

Performance data

Linear Range and Sensitivity (Total RNA)

Figure 2. Broad linear range and high sensitivity

Inter-Array Reproducibility

Figure 3. High inter-array reproducibility 
Single Nucleotide Mismatches

Figure 4. Specificity of miRNA detection