Monthly Archives: February 2019

GeneHero™ Cas9 Nuclease

Recombinant Streptococcus pyogenes Cas9 nuclease, purified from E. coli, is a great choice for genome editing applications in vitro or in vivo. Available in two versions, containing nuclear localization sequence (NLS), and either with or without a C-terminal 6XHis-tag, at a concentration of 1 mg/ml.  For optmal activity, use with GeneCopoeia's CRISPR-Fectin™ Transfection Reagent. Order information Product details Resources Related products Order information All Cas9 nucleases are dissolved in storage buffer at 1 mg/ml. Buy Catalog# Size ......
Posted in Genome editing tools

BlazeTaq™ First-Strand cDNA Synthesis Kit

A robust experimental design delivers a universal kit suitable for first-strand cDNA synthesis from most any source of RNA Efficient and easy Reliable Cost efficient The BlazeTaq™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification. The kit uses the Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H–)) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with l......
Posted in qPCR products

GeneHero™ CRISPRi Stable Cell Lines

GeneHero™ CRISPRi Stable Cell Lines are mammalian cell lines stably expressing dCas9-KRAB fusion protein for the study of transcriptional inhibition of endogenous genes. These CRISPRi cell lines have stably integrated human codon-optimized dCas9 fused to a transcriptional repressor peptide KRAB to silence target gene expression. The cell lines are monoclonal with verified dCas9-KRAB genome integration and expression. Simply introduce promoter-targeting sgRNA or CRISPRi sgRNA libraries into the CRISPRi cell lines. They are ideal for the study of gene expression inhibiti......
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Wild type Parental HCT116 Reference Standard

The Wild type Parental HCT116 Reference Standard is genomic DNA derived from a highly characterized diploid HCT116 cell line. The product can be mixed with single-point mutation reference standard to reach the target ideal variant allele frequency (VAF). This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {background-color: #f9f9f9; color: black; cursor: pointer; margin:0px; padding: 10px; width: 100%; border-color:#c1c1c1; border-width:2px; border-......
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AKT E17K Reference Standard

The AKT E17K Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation E17K in AKT on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {background-color: #f9......
Posted in Products

cKIT D816V Reference Standard

The cKIT D816V Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation D816V in cKIT on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {background-color:......
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BRAF V600E Reference Standard

The BRAF V600E Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation V600E in BRAF on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {background-color:......
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KRAS G13C Reference Standard

The KRAS G13C Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation G13C in KRAS on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {background-color: #......
Posted in Products

EGFR T790M C797S Reference Standard

The EGFR T790M C797S Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation T790M and C797S in EGFR on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {b......
Posted in Products

EGFR T790M Reference Standard

The EGFR T790M Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation T790M in EGFR on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {background-color:......
Posted in Products