DNase I

by shaoqunxian | Jul 19, 2024 | Reagents and Kits

DNase I DNase I

Price:	$54.00 $216.00
Catalog#:	PC024 PC025
XX:	1,000 U 5,000 U
Size:	1,000 U 5,000 U
Qty:
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Product Information DNase I is an endonuclease that digests single- and double- stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5’-phosphate and 3’-OH groups. The enzyme activity is strictly dependent on Ca²⁺ and is activated by Mg²⁺ or Mn²⁺ ions. If in the presence of Mg²⁺, DNase I cleaves each strand of dsDNA independently, in a statistically random fashion. If in the presence of Mn²⁺, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with one or two nucleotide overhangs.

Applications

Preparation of DNA-free RNA samples
Removal of DNA contamination from RNA samples prior to RT-qPCR reaction
DNA template after removal of RNA transcription in vitro
DNase I footprinting assay
Construction of DNA random fragment library

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T4 DNA Ligase

by shaoqunxian | Jul 19, 2024 | Reagents and Kits

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T4 DNA Ligase T4 DNA Ligase

Price:	$57.00 $208.00
Catalog#:	FF004 FF005
XX:	20,000 U 100,000 U
Size:	20,000 U 100,000 U
Qty:
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Product Information T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini. The enzyme has no binding effect on single stranded DNA or RNA.

Order information

Cat. No.	product name	enzyme concentration	specification
FF004	T4 DNA Ligase	400U/μl	20,000 U, 50 μL
FF005	T4 DNA Ligase	400U/μl	100,000 U, 5 x 50 μL
inquire	T4 DNA Ligase（high concentration）	＞1,500 U/μL

*For T4 DNA ligase (high concentration, > 1,500 U/μL) , please contact us at 1-301-762-0888 or inquiry@genecopoeia.com.

Applications

Cloning of restriction enzyme fragments
DNA fragments with blunt end join Adapters
Ligase mediated RNA detection
Notch repair in double helix DNA hybrids
Self-cyclization of linear DNA

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AccelerRT® Ⅲ Reverse Transcriptase

by shaoqunxian | Jul 19, 2024 | Reagents and Kits

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AccelerRT® III Reverse Transcriptase AccelerRT® III Reverse Transcriptase

Price:	$64.00 $260.00
Catalog#:	PC026 PC027
XX:	10,000 U 50,000 U
Size:	10,000 U 50,000 U
Qty:
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Product Information AccelerRT^® Ⅲ Reverse Transcriptase (RT) is an engineered version of M-MuLV RT with reduced RNase H activity. RNase H specifically degrade the RNA stand in RNA-DNA hybrids, therefore it probably degrade the RNA template in RNA-DNA hybrids when the first-stand cDNA synthesis. The enzyme can be used to synthesize more full-length product than conventional M-MLV, stronger extension ability. And it can used to Real Time RT-PCR.

Advantages

For longer cDNA synthesis
Active temperature up to 65°C

Applications

Reverse Transcription
First-stand cDNA synthesis
RNA virus detection(cDNA synthesis)
RT-qPCR

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T7 RNA Polymerase

by 锘妍黄 | Jul 2, 2024 | Reagents and Kits

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T7 RNA Polymerase T7 RNA Polymerase

Price:	$76.00 $303.00
Catalog#:	PC028 PC029
XX:	5,000 U 25,000 U
Size:	5,000 U 25,000 U
Qty:
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Product Information

T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for T7 promoter. Use double-stranded DNA (linearised plasmid or PCR product) as a template to synthesise RNA that is complementary to the reverse single-stranded DNA downstream of the promoter. T7 RNA Polymerase accepts modified nucleotides (e.g., biotin-, digoxigenin-, fluorescein-labeled nucleotides) as substrates for RNA synthesis.

Catalog#	Product	Size
PC028	T7 RNA Polymerase (50 U/μL)	100 μL
PC028	10× Transcription Buffer	250 μL
PC029	T7 RNA Polymerase (50 U/μL)	5× 100 μL
PC029	10× Transcription Buffer	5× 250 μL
Inquire	T7 RNA Polymerase ( >200 U/μL)

*For T7 RNA polymerase (high concentration, >200 U/μL) products, please contact us at 1-301-762-0888 or inquiry@genecopoeia.com.

Storage buffer: 50 mM Tris-HCl，100 mM NaCl，20 mM β-ME，1 mM EDTA，50% Glycerol，0.1% (w/v) Triton® X-100 (pH 7.9 @ 25°C)

Definition of activity unit: One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C.

Advantages

Yields 150~200 μg of RNA from 1 μg of DNA template.
Optimized transcription buffer: Requires less T7 RNA polymerase for maximum RNA yield compared to other competitors.

Application

Synthesis of large amounts of RNA, including:

mRNA for vaccines or gene expression
circRNA precursors
Modified RNA: prepared by admixing modified NTP (e.g., aminoallyl-, biotin-, fluorescein-, digoxin-NTP)
labelled RNA probes, etc.

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DNase I DNase I

Applications

Different temperatures to digest DNA

Figure 1.The pUC19 plasmid was digested using DNase I at 25 ° C /10min and 37 ° C /10min, followed by 1% agar-gel electrophoresis.

Compare digestibility

Figure 2.Using PC024(red) and Competitor DNase I(blue) to digest 20pg to 200ng 293T total RNA containing gDNA residues, followed by qPCR amplification using BlazeTaq™ SYBR® Green qPCR mix 2.0(Cat.# QP031).

RNA stability after digestion

T4 DNA Ligase T4 DNA Ligase

Order information

Applications

End-joining reaction

Figure 1.The adhesive end substrate was attached using T4 DNA Ligase at 25 ° C, 10/20/30 mins, followed by 1% agarose gel electrophoresis.

Carrier construction application

Figure 2. Using T4 DNA Ligase to clone the target fragments to the vector, and then transformed into DH5a receptor cells. After transformation and culture, single colonies containing target genes were screened by PCR analysis.

DNA notch repair application

AccelerRT® III Reverse Transcriptase AccelerRT® III Reverse Transcriptase

Advantages

Applications

Exceptional RNA template sensitivity

Figure 1.First-strand cDNA was generated from 100 ng to 1 pg of total RNA from Hela total RNA and Jurkat total RNA using AccelerRT® Ⅲ Reverse Transcriptase. The synthesized cDNA was used as a template for qPCR using All-in-One™ qPCR Mix(Cat.# QP001).

Long fragment amplification

Consistent performance in the presence of a variety of inhibitors

Figure 3. Various inhibitors were added to total 293T total RNA, then was used in a reverse transcription reaction with AccelerRT® Ⅲ Reverse Transcriptase, The synthesized cDNA was used as a template in subsequent qPCR using All-in-One™ qPCR Mix(Cat.# QP001).

T7 RNA Polymerase T7 RNA Polymerase

Application

T7 RNA Polymerase reaction time course

Transcription products in different length

Figure 2. The in vitro transcription reactions were carried out by adding 250 ng of different sized template to a 20 µL reaction. As shown, the T7 RNA Polymerase is suitable for in vitro transcription reactions with different product lengths.

5′-TAATACGACTCACTATAG-3′

T7 RNA polymerase starts transcription from the underlined G in the promoter sequence. Using the reverse strand as a template, transcription begins at 5′->3′. The first base in the transcript will be G.

Taq DNA Polymerase Kit (Cat. No. PC003)

Super Taq DNA Polymerase Kit (Cat. No. C0102A)

UltraHiPF® DNA Polymerase Kit (Cat. No. PC018)

Custom clone construction for IVT(Coming Soon! )

RNaseLock™ RNase Inhibitor (Cat. No. PC005)

DNase I (Cat. No. PC024)

T7 High Yield RNA Synthesis Kit (Coming Soon! )

Custom circRNA synthesis service (Coming Soon! )

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