Product Information
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini. The enzyme has no binding effect on single stranded DNA or RNA.
Order information
| Cat. No. |
product name |
enzyme concentration |
specification |
| FF004 |
T4 DNA Ligase |
400U/μl |
20,000 U, 50 μL |
| FF005 |
T4 DNA Ligase |
400U/μl |
100,000 U, 5 x 50 μL |
| inquire |
T4 DNA Ligase（high concentration） |
＞1,500 U/μL |
|
*For T4 DNA ligase (high concentration, > 1,500 U/μL) , please contact us at 1-301-762-0888 or
inquiry@genecopoeia.com.
Applications
- Cloning of restriction enzyme fragments
- DNA fragments with blunt end join Adapters
- Ligase mediated RNA detection
- Notch repair in double helix DNA hybrids
- Self-cyclization of linear DNA
