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Resources for Antigen MicroArray

by | Oct 28, 2024 | Resources

Technology overview

Technology overview

GeneCopoeia’s OmicsArray™ antigen microarrays contain up to 120 purified antigens spotted onto nitrocellulose filters, which are adhered to glass slides. In addition, 8 spots are included for normalization. Each slide carries 16 identical arrays, and so can be used to process up to 15 samples simultaneously as well as a negative control. As little as 1 μL serum or 50 μL of other biofluids is needed for each sample.
As shown in Figure 1, arrays are incubated with patient samples, and any autoantibodies in the samples bind to their cognate antigens on the array. The arrays are washed to remove unbound autoantibodies and other proteins, then co-incubated with Cy3- and Cy5-labeled secondary antibodies. The dual labeling strategy is intended to distinguish between immunoglobulin (Ig) subtypes present within samples. For example, a Cy3-labeled anti-IgG secondary antibody is used to detect IgG autoantibodies, and a Cy5-labeled anti-IgM secondary antibody is used to detect IgM autoantibodies. Fluorophore-labeled secondary antibodies are available for detecting IgA, IgD, IgE, IgG and IgM immunoglobulins, as well as IgG subclasses IgG1, IgG2, IgG3, and IgG4.
After washing to remove unbound secondary antibodies, signals are detected using a microarray scanner (e.g., GenePix® 4000B, InnoScan 710, or equivalents). The raw data is then analyzed using GenePix® Pro 7.0 or Mapix software.

Workflow for autoantibody profiling in samples using GeneCopoeia’s OmicsArray™ antigen microarrays.

Figure 1. Workflow for autoantibody profiling in samples using GeneCopoeia’s OmicsArray™ antigen microarrays.

Fast-Fusion™ Multi Seamless Cloning Kit

by | Oct 24, 2024 | Products

Fast-Fusion™ Multi Seamless Cloning Kit
Fast-Fusion™ Multi Seamless Cloning Kit
Fast-Fusion™ Multi Seamless Cloning Kit
Fast-Fusion™ Multi Seamless Cloning Kit

Fast-Fusion™ Multi Seamless Cloning Kit Fast-Fusion™ Multi Seamless Cloning Kit

Price: $352.00 $806.00
Catalog#:
  • FF006
  • FF007
Size: 20rxns 60rxns
Qty:
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Introduction

For rapid and effective cloning of PCR products

The GeneCopoeia Fast-Fusion™ Multi Seamless Cloning Kit provides a rapid method for cloning your PCR products. In as little as 5 minutes minutes at 50℃, any PCR fragment can be cloned into your linearized vector. After a simple clean up step, a PCR-generated DNA fragment or other purified DNA fragment can be joined to a vector with overlapping ends (Fig.A).Up to five DNA fragments can be joined together in a single reaction. A single fragment can be efficiently fused and assembled in 5 minutes. Well-prepared vectors generate almost 100% positive clones.
No restriction sites are needed at the junction, enabling insertion of your fragment of interest at any vector position. The linearized vector can be generated by either PCR or restriction enzyme digestion. The PCR products can be produced by either Taq DNA polymerase or other high fidelity DNA polymerase.

Advantages

  • Fast and simple—1 minute for operation and5 minutes for incubation.
  • High adaptability—No restriction or recombination sites needed, insert fragments generated by either PCR or restriction enzyme digestion can be used.
  • Seamless construction—Final constructs are seamless with no extra or unwanted base pairs.
  • Flexibility—Multiple inserts can be assembled in one reaction. Suitable for multi-site mutagenesis.
  • High efficiency—Greater than 90% of colonies after transformation contain the correct insert(s).
 

Portocol overview

Figure A. Experimental workflow of using the Fast-FusionTM  Multi Seamless Cloning Kit

T7 RNA Synthesis Kit

by | Oct 21, 2024 | Reagents and Kits

T7 RNA Synthesis Kit
T7 RNA Synthesis Kit
T7 RNA Synthesis Kit
T7 RNA Synthesis Kit

T7 RNA Synthesis Kit T7 RNA Synthesis Kit

Price: $270.00 $460.00
Catalog#:
  • PC035
  • PC036
Size: 50 Reactions 100 Reactions
Qty:
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Introduction T7 RNA Synthesis Kit is designed for in vitro transcription of large amounts of RNA, provides with T7 RNA polymerase mix and four individually available nucleotides. The kit can also be used to synthesise capped RNA by adding cap analogues; or to synthesise modified RNA by replacing with modified UTP according to experimental requirements. The kit contains LiCl Solution for removing free nucleotides, enzymes and most of the template from the transcription product to obtain coarsely purified RNA.

Advantage

  1. Yields 150~200 μg of RNA from 1 μg of DNA template.
  2. Optimized transcription buffer: Requires less T7 RNA polymerase for maximum RNA yield compared to other competitors.

Application

Synthesis of large amounts of various RNAs, including but not limited to:
  1. mRNA for vaccine or gene expression
  2. precursors of circular RNA
  3. labeled RNA probes
  4. Modified RNA: prepared by admixing modified NTP (e.g., aminoallyl-, biotin-, fluorescein-, digoxin-NTP)