Monthly Archives: November 2014

GeneHero™ CRISPR human sgRNA libraries

Introduction Applications To order Related Products Resources Introduction GeneCopoeia offers CRISPR sgRNA libraries for high-throughput knockout of human genes in specific or custom gene groups or pathways. Loss-of-function screening by gene knockout is a powerful tool for systematic genetic analysis in mammalian cells, facilitating gene discovery, genome-scale functional interrogation (e.g. signal transduction pathways) and drug discovery (e.g. target identification and drug mechanism studies). The GeneHero™ human single guide RNA (sgRNA) librarie......
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Lentivirus: That’s My MOI, and I’m Sticking To It

  Ed Davis, Ph.D. Introduction Lentiviral vectors have proven invaluable for introducing genetic material into mammalian cells, either in culture or whole animals (reviewed in Mátrai, et al., 2010). Lentiviruses, primarily derived from human or feline immunodeficiency viruses (HIV or FIV, respectively), infect most cell types, including those that are difficult-to-impossible to transfect with plasmids. GeneCopoeia offers every one of our human and mouse ORF, promoter, shRNA, precursor microRNAs, microRNA inhibitor, and CRISPR sgRNA clones in lentivira......
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ORF clones: many tags, many decisions

  Ed Davis, Ph.D. Introduction Fusion tagging-the practice of adding amino acids in-frame to one end of a native protein-is a well- established strategy for many applications, including protein purification, immunoprecipitation, Western blotting, and in vivo imaging. GeneCopoeia provides tens of thousands of human and mouse open reading frame (ORF) clones for ready expression in vitro and in vivo. These clones come either without tags or with a large number of fusion tag options for customers to choose from. However, designing a tagging strategy r......
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