Monthly Archives: January 2015

Genome Editing: HDR Donors For Gene Knockout, Mutagenesis, Tagging, and Safe Harbor Knock-in

  Ed Davis, Ph.D. Genome Editing—making specific changes at targeted genomic sites—is fundamentally important to researchers in biology and medicine (Bogdanove & Voytas, 2011; van der Oost, et al., 2013). Two popular genome editing technologies exploit bacterial systems for adaptive immunity or plant pathogenesis: CRISPR (Clustered, Regularly Interspaced, Short Palindromic Repeats), and TALEN (Transcription Activator-Like Effector Nucleases) and), respectively.  Both initiate double-strand breaks (DSBs) at virtually any genomic s......
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