Applications of OmicsLink™ Clones and Comparison with Whole Transcript cDNA Clones

Express proteins with unique tag that facilitates purification and down-stream analysis
Generate probes of in situ hybridization for tissue or organ gene expression profiling
Produce templates for esiRNA for gene silencing functional analysis
Validation of shRNA/miRNA function

Clone Characteristics	OmicsLink™ ORF clones	Whole Transcript cDNA Clones
Gene transcript	ORF (Start to stop only)	Whole transcripts including 5' and 3' UTRs
Expression ready	Yes	Some are, most will need further subcloning procedures to remove 5' and 3' UTRs
Mammalian cell transfection – Transicent Transfection – Stable Transfection	Yes Yes	Only a small number No
Unique protein tags – Protein-Molecule interactions – Concentration of lowly expressed proteins – Affinity purification of expressed proteins	Yes Yes Yes Yes	No No No No
shRNAi validation and rescue	Yes	No
miRNA and miRNA target validation and rescue	Yes	No
*Produce proteins via in vitro* cell-free translation systems**	Yes	No
production Template for esiRNA	Yes	No
*Probe generations for in situ* hybridization**	Yes	No