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Product Information
T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for T7 promoter. Use double-stranded DNA (linearised plasmid or PCR product) as a template to synthesise RNA that is complementary to the reverse single-stranded DNA downstream of the promoter. T7 RNA Polymerase accepts modified nucleotides (e.g., biotin-, digoxigenin-, fluorescein-labeled nucleotides) as substrates for RNA synthesis.
Storage buffer: 50 mM Tris-HCl，100 mM NaCl，20 mM β-ME，1 mM EDTA，50% Glycerol，0.1% (w/v) Triton® X-100 (pH 7.9 @ 25°C) Definition of activity unit: One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C.
Advantages
- Yields 150~200 μg of RNA from 1 μg of DNA template.
- Optimized transcription buffer: Requires less T7 RNA polymerase for maximum RNA yield compared to other competitors.
Application
Synthesis of large amounts of RNA, including:- mRNA for vaccines or gene expression
- circRNA precursors
- Modified RNA: prepared by admixing modified NTP (e.g., aminoallyl-, biotin-, fluorescein-, digoxin-NTP)
- labelled RNA probes, etc.
