Product Introduction
A robust experimental design delivers a universal kit suitable for first-strand cDNA synthesis from most any source of RNA
- Efficient and easy
- Reliable
- Cost efficient
The BlazeTaq™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification. </p > The kit uses the Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H–)) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.
Product Details
BlazeTaq™ First-Strand Synthesis Kit in action
The BlazeTaq™ First Strand Synthesis Kit can be used in combination with the BlazeTaq™ SYBR Green qPCR Master Mix as the first step of the two-step RT-qPCR system to convert mRNA into cDNA; the cDNA can be subsequently aliquoted, stored or used for qPCR analysis.
Figure 1. Amplification of actin-β using the BlazeTaq™ two-step RT-qPCR system. A 10-fold dilution series of RNA extracts from HeLa cells were used for cDNA conversion with the BlazeTaq™ First-Strand cDNA Synthesis Kit. The actin-β cDNA was amplified with BlazeTaq™ SYBR® Green qPCR mix 2.0 with amounts ranging from ~100 ng to ~1 pg.
Figure 2. Comparison of the real-time amplication plots of the BlazeTaq Two-Step RT-qPCR System (red) with competitor A’s (A) and competitor B’s (B) products (blue). A 10-fold dilution series of B2M gene from total RNA of Hela cell extract with amounts ranging from 100 ng to 1 pg.
