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Product Information
DNase I is an endonuclease that digests single- and double- stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5’-phosphate and 3’-OH groups. The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions. If in the presence of Mg2+, DNase I cleaves each strand of dsDNA independently, in a statistically random fashion. If in the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with one or two nucleotide overhangs.
Applications
- Preparation of DNA-free RNA samples
- Removal of DNA contamination from RNA samples prior to RT-qPCR reaction
- DNA template after removal of RNA transcription in vitro
- DNase I footprinting assay
- Construction of DNA random fragment library
