Introduction
Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. DBCO alkynes can be used to perform click reactions with azide-modified targets without the use of heavy metal catalysis. DBCO reactions are ideal for surface labeling of live cells and also minimize damage to fluorescent proteins like GFP or R-PE.
The Cy5 DBCO is reactive with azide via a Strain-promoted Azide-Alkyne Click Chemistry reaction (SPAAC).
Features
- Efficiency—the click reaction is complete in less than 1 hour;
- Specificity—the reaction between the label and detection tag is selective and specific;
- Stability—the reaction product contains an irreversible, covalent bond;
- Biologically inert—the components of the reaction do not undergo any side reactions.
Specifications
| Label | Cy5 |
| Ex/Em | 650/665 |
| Detection Method | Fluorescent |
| Solubility | DMSO, DMF |
| Molecular Weight | 1044.38 |
| Product Size | 0.5 µmol |
| Storage Conditions | -20 ℃, protect from light |
| Shipping Condition | Room Temperature |
| Applications | Click chemistry labeling |