FAQs

How do you do quality control of your clones?

Answer: During the production of an ORFEXPRESS™ shuttle clone, each full length ORF clone delivered to you is fully sequenced. Before the delivery of each clone, we perform additional QC by PCR with gene specific primers to ensure that you are...

I cannot find the gene(s) of my interest from searching the database in your website. Can you clone the gene(s) for me? How much will it cost?

Answer: Yes, we will clone the gene(s) for you. It is our mission and continuing effort to clone all full-length ORFs of human genes. It is likely that the gene(s) that you are interested in is already in our production pipeline or in the queue for future cloning. If...

In some cases, when comparing your reference sequences with those from NCBI with the same gene symbol or description, I notice there are differing degrees of discrepancies between the sequences. Can you explain the discrepancies?

Answer: There are usually multiple gene transcripts in a gene locus, resulting from alternative splicing, updated information about ORFs, polymorphism, or redundancies from duplication of cloning effort from different laboratory groups. During the process of selecting...

In the annotation pages for your genes/clones, is the sequence on your website the actual sequence of your clone?

Answer: No, the sequence on the website is not the actual sequence but the reference sequence from NCBI. The actual sequence of the clone may differ due to genetic variation and alternative splicing. The actual sequence for any of the ORF cDNA clones can be requested...

There is one nucleotide change in the sequence, and it encodes an amino acid for proper protein function as sited by many literatures, how will you handle this case?

FAQs

Answer: Even though we cannot provide replacement clones for this type of cases, we do offer mutagenesis services to change any nucleotide compositions as requested for a nominal fee.

Since you use PCR method to generate full length ORFs, how will you handle PCR induced mutation?

FAQs

Answer: Our system incorporated a new high fidelity PCR composition that improves fidelity in generating full length ORFs by 10 fold. It is accomplished by reducing the non-specific binding of...

How do you explain the differences between your actual sequencing data and GenBank sequences?

FAQs

Answer: The differences can originate from two potential sources or combination of the two: 1) naturally occurring events including polymorphisms, alternative splicings, inconsistencies in multiple...

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