*Promotions are valid in the US & Canada only. For international customers, please contact your local distributors. Promotion ends July 31, 2025. Discounts are not valid on previous purchases and cannot be combined with additional discounts.
A robust experimental design delivers a universal kit suitable for first-strand cDNA synthesis from almost any source of RNA
- Efficient and easy
- Reliable
- Cost efficient
The SureScript™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification.
The kit uses the Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H–)) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.