LXRa transcriptional response element (TRE) Clone

LxRa transcriptional response element (TRE) clone for LXR signaling pathway
LxRa transcriptional response element (TRE) clone for LXR signaling pathway
Secreted alkaline phosphatase (SEAP)<br />Expression clone
Secreted alkaline phosphatase (SEAP)
Expression clone
Transcriptional response element(TRE) negative control clone
Transcriptional response element(TRE) negative control clone
GAPDH positive control clone
GAPDH positive control clone

LxRa transcriptional response element (TRE) clone for LXR signaling pathway Secreted alkaline phosphatase (SEAP)
Expression clone
Transcriptional response element(TRE) negative control clone GAPDH positive control clone

Price: $364.00 $411.00 $364.00 $390.00
Catalog#:
  • TR114
  • SEAP-PA01
  • TR001
  • GAPDH-PG02
Size: pEZX-PG02 pEZX-PA01 pEZX-PG02 pEZX-PG02
Qty:
Manual: DownloadDownloadDownload
View your cart


Introduction

GeneCopoeia’s GLuc-ON™ LXRa transcriptional response element (TRE) clone enables analysis of the transcriptional activity of liver X receptor (LXR). LXRs comprise 2 members: LXRa and LXRb. LXRs are nucleus-localized transcription factors that bind oxidized forms of cholesterol and are critical players in the regulation of cholesterol levels.

The GLuc-ON™ LXRa TRE clone contains tandem repeats of LXRa upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The LXRa response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ LXRa TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activated) and normalization (secreted alkaline phosphatase) control clones are also available for purchase.

User Manual
 

Validation Data

    Figure 1. Activation of the GLuc-ON™ LXRa TRE by exogenous expression of LXRa. 293T cells were co-transfected with (left to right) negative TRE clone + negative expression clone, negative TRE clone + LXRa expression clone, LXRa TRE clone + negative expression clone, or LXRa TRE clone + LXRa expression clone. Transfected cells were incubated for 40 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.