CRISPR Insertion or Deletion Detection System

IndelCheck™ CRISPR/TALEN insertion or deletion detection system
Includes target site PCR kit (IC003) and T7 endonuclease I assay kit (IC005)
IndelCheck™ CRISPR/TALEN insertion or deletion detection system
Includes target site PCR kit (IC004) and T7 endonuclease I assay kit (IC006)
Target site PCR Kit (version 2.0)
PCR reagents for amplifying region flanking CRISPR/TALEN target site, prior to T7 Endonuclease I digestion*
Target site PCR Kit (version 2.0)
PCR reagents for amplifying region flanking CRISPR/TALEN target site, prior to T7 Endonuclease I digestion*
T7 endonuclease I assay kit
Cleave mismatched PCR products using T7 endonuclease I to detect indel mutations
T7 endonuclease I assay kit
Cleave mismatched PCR products using T7 endonuclease I to detect indel mutations
Smart-Join™ Target Site PCR Cloning Kit
Smart-Join™ Target Site PCR Cloning Kit, 20 reaction
Smart-Join™ Target Site PCR Cloning Kit
Smart-Join™ Target Site PCR Cloning Kit, 100 reaction

IndelCheck™ CRISPR/TALEN insertion or deletion detection system IndelCheck™ CRISPR/TALEN insertion or deletion detection system Target site PCR Kit (version 2.0) Target site PCR Kit (version 2.0) T7 endonuclease I assay kit T7 endonuclease I assay kit Smart-Join™ Target Site PCR Cloning Kit Smart-Join™ Target Site PCR Cloning Kit

Price: $214.00 $647.00 $165.00 $498.00 $103.00 $311.00 $259.00 $727.00
Catalog#:
  • IC001
  • IC002
  • IC003
  • IC004
  • IC005
  • IC006
  • IC007
  • IC008
Size: 50 rxns 200 rxns 50 rxns 200 rxns 50 rxns 200 rxns 20 reaction 100 reaction
Qty:
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Product Information

GeneCopoeia’s IndelCheck™ CRISPR insertion or deletion (indel) detection system provides a powerful means for assisting you in your genome editing applications. The IndelCheck™ system can be used for:

  • CRISPR sgRNA functional validation (Figure 1) before undertaking long genome editing projects (3-6 months for genome edited cell lines, or 6-9 months for genome-edited mouse lines), saving you a great deal of time and effort by eliminating CRISPR sgRNAs with poor cleavage efficiency.
  • Screening cell clones for knockout (KO) and knock-in (KI) modifications (Figure 2).

Advantages

  • Complete system to simplify your CRISPR validation and edited clone screening
  • Robust amplification for the target site PCR. No genomic DNA isolation is required
  • Easy to use T7 endonuclease I assay with optimized conditions and positive control

 

Order Information

The IndelCheck™ system consists of the following major components:

  1. Target site PCR kit V2.0. For amplification of targeted genomic regions from cell lysates without genomic DNA isolation. Now in Version 2.0, the Target Site PCR kit contains buffer, SuperHeRo™ DNA polymerase, and nucleotides together in one mix for greater convenience compared with Version 1.0
  2. T7 endonuclease I assay kit. For the detection of CRISPR-introduced indel mutations near target site(s).
  3. Smart-Join™ Blunt-end PCR Cloning Kit. For cloning of PCR products to be used for sequencing and identification of CRISPR-mediated genomic modifications.

 

Related Products

Validation service

GeneCopoeia also offers CRISPR validation services using the IndelCheck™ CRISPR insertion and deletion detection system. We can validate the CRISPR you purchased from us using HEK293 cells (human), NIH3T3 cells (mouse), or your specific cell line(s). Please contact us at inquiry@genecopoeia.com or 301-762-0888.

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