RACE (Rapid Amplification of cDNA Ends) is a technique that enables rapid amplification of the unknown sequences at the end of the cDNA, using known partial sequences as an anchor. The AccelerRT® 5′ RACE Kit enables efficient reverse transcription of cDNA from 10 ng to 1 μg of total RNA, followed by rapid amplification of the 5′ ends using the synthesized cDNA as the template.

In 5′ RACE, upon reaching the 5′ end of the RNA template, a specific adapter sequence is annealed and extended to the 3′ end of the cDNA by the terminal deoxynucleotidyl transferase (TdT) activity of the Template Switching Reverse Transcriptase, eliminating the need for separate adapter ligation steps. The PCR amplification system utilizing the 2× PCR Amplification Mix enables amplification of long fragments and GC-rich regions, while maintaining high specificity to minimize smearing in RACE experiments.

This kit provides all pre-optimized reagents for 5′ RACE. Altering component concentrations or substituting with equivalent products is not recommended and may yield suboptimal results. Prior validation is required if substitution is necessary.

Advantages

• Streamlined procedure with no adapter ligation steps required
• 5′ RACE RT Mix delivers robust reverse transcription and template-switching activity for efficient 5′ RACE fragment generation
• 2× PCR Amplification Mix offers high amplification efficiency and fidelity to obtain accurate target fragments
• Compatible with 10 ng–1 μg of total RNA or poly(A)+ RNA as input template