FAQs

Since you use PCR method to generate full length ORFs, how will you handle PCR induced mutation?

Answer: Our system incorporated a new high fidelity PCR composition that improves fidelity in generating full length ORFs by 10 fold. It is accomplished by reducing the non-specific binding of primers with template DNA at lower reaction temperature and by reducing the...
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How do you explain the differences between your actual sequencing data and GenBank sequences?

Answer: The differences can originate from two potential sources or combination of the two: 1) naturally occurring events including polymorphisms, alternative splicings, inconsistencies in multiple copies/versions of the same genes resulting from annotation updates,...
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How do you generate full length ORF to produce your clones?

Answer: We use PCR together with several patented technologies to maximize the fidelity of PCR to generate full length ORF (more information in ORF White Paper). We used more than 70 cDNA libraries of human or mice tissues as templates to generate full length ORFs....
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I would like to sequence the ORFs, what the primer sequences can I use?

Answer: For the ORFEXPRESS™ Shuttle clones, the forwarding sequencing primer (from upstream of 5' end of ORF) is: 5'-CCCAGTCACGACGTTGTAAAACG, and the reverse sequencing primer (from downstream of 3' end of ORF) is: 5'-ATGGTCATAGCTGTTTCCTG. For...
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Have you done expression test of each gene?

Answer: All expression clones in pReceiever-B01 and pReceiver-WG02/WG03/WG04/WG05/WG09/WG16 have been tested for expression in E. coli or cell free protein production system made by Roche. Other...

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