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Lenti-Pac™ Lentiviral Packaging Systems

Introduction

Lentiviral Packaging Systems

The GeneCopoeia Lenti-Pac™ HIV Expression Packaging Systems includes an optimized lentiviral packaging plasmid mix, an eGFP positive control plasmid, a transfection reagent, EndoFectin™ Lenti (optimized for virus production), and TiterBoost™ reagent that further increases the titers 5-10 fold. When combined with GeneCopoeia lentiviral constructs, the results are high viral titers and robust expression levels.

 

The packaging plasmids in both HIV packaging systems are designed for coordinated, high-level expression of all essential lentiviral packaging elements to ensure efficient packaging of the recombinant transcript into infectious virus particles, as well as for preventing the viral sequences from self-replicating.

 

Lentiviral 293Ta packaging cell line

GeneCopoeia also offers 293Ta packaging cells for high titer lentivirus production using optimized packaging plasmids with lentiviral vector-based ORF cDNA, shRNA or miRNA constructs. Lenti-Pac 293Ta packaging cell line enables easy and efficient transfection for high-level expression of viral proteins required for packaging.

 

Lentiviral Concentration Solution

The Lenti-Pac™ Lentivirus Concentration Solution is designed for quick and simple concentration of lentiviral particles. The concentration is accomplished by mixing the proprietary reagent with the lentiviral supernatant, followed by a short incubation and centrifugation with an ordinary centrifuge.

 

Lentiviral Titration Kits

The Lenti-Pac™ HIV qRT-PCR Lentivirus Titration Kits are designed for quick and simple titration of lentiviral particles to determine the virus copy numbers and to confirm the success of packaging reactions.  The kits include the RNA extraction reagent, qRT-PCR reagents and standard control. The RNA genome of lentivirus is reverse-transcribed and quantified using qRT-PCR and SYBR® green technologies.

To Order Lentiviral Systems

 
Buy Catalog# Product Description Manual or datasheets Price
HPK-LvTR-20 Lenti-Pac HIV Expression Packaging Kit (20 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent HIV packaging kit manual $592
HPK-LvTR-40 Lenti-Pac HIV Expression Packaging Kit (40 reactions) HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent HIV packaging kit manual $989
HPR-LTK-050 Lenti-Pac HIV qRT-PCR Titration Kit (20 RT reactions, 50 PCR reactions) qRT-PCR based titration to determine the copy numbers of HIV lentiviral particles. Lentivirus titration kit manual $285
HPR-LTK-100 Lenti-Pac HIV qRT-PCR Titration Kit (40 RT reactions, 100 PCR reactions) qRT-PCR based lentiviral titration to determine the copy numbers of HIV lentiviral particles. Lentivirus titration kit manual $430
LPR-LCS-01 Lenti-Pac™ Lentivirus Concentration Solution (50 ml) Quick and simple concentration of lentiviral particles Lentivirus concentration solution manual $139
CLv-PK-01 Lenti-Pac 293Ta Cell Line (1.5 x 106 cells) 293Ta Lentiviral packaging cell line 293Ta packaging cell line datasheet $250
MP001 Mycoplasma PCR detection kit (50 rxns) Quick detection of mycoplasma contamination in cell culture or virus Mycoplasma PCR Detection Kit $125

 
 

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