Introduction
E3 ubiquitin ligases are key components of the ubiquitin–proteasome system (UPS), selecting and tagging target proteins for degradation or regulation. They are responsible for selecting and labelling target proteins for degradation or regulation. The UPS system is the main mechanism by which cells degrade proteins. Its core function involves the coordinated action of E1s, E2s and E3 ubiquitin ligases, which work together to attach ubiquitin molecules to target proteins and mark them for proteasomal degradation. E3 ligases recognise specific substrate proteins and transfer ubiquitin to lysine residues on these proteins. With over 600 members, they control vital processes such as cell cycle, DNA repair, and immune signalling. Dysregulated E3 activity drives cancer, neurodegeneration, and inflammation, making E3s important targets for mechanistic studies and drug development.Genecopoeia offers sgRNA targeting E3 ubiquitin ligases for mechanistic studies and drug development.