Select TALEN / CRISPR-Cas9 Expression Clones Search again

Product ID:

HTN296965 (click here to view gene annotation page)

Species:

Human

Symbol:

NEDD4L

Alias:

NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2

Gene Description:

NEDD4 like E3 ubiquitin protein ligase

Target Gene Accession:

NM_015277.5(click here to view gene page)

Delivery format:

10 μg purified plasmid

Estimated Delivery:

Approximately 2-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.

Read Warranty Statement

Important Note:

By default, the TALEN/CRISPR constructs are designed to target the protein coding-region as closely as possible to the initiator ATG of the splice variant (accession number) you selected. This design strategy does not consider other possible variants of this gene. If this gene has multiple variants and you would like to target one particular exon, one unique variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.

Note:

One or more of the sgRNA target sites for this gene has 100% DNA sequence identity with chromosomal sequences elsewhere in the reference sequence genome. This is likely due to gene duplication. Such sgRNAs are expected to modify these identical off-target sites, although it is possible to identify clones with only the intended target modified. For questions or concerns, please contact support@genecopoeia.com.

CRISPR
HDR Donor Clones
HDR Donor Vectors
Indel Detection
Complete Solutions

The CRISPR sgRNA clone options listed below are intended for use as 20 nt sgRNAs with the wild-type Cas9 nuclease from Streptococcus pyogenes (SpCas9). GeneCopoeia does provide alternate CRISPR options, such as Cas9 D10A nickase and other high-fidelity versions of Cas9, SaCas9, and shorter sgRNAs. To purchase reagents for these other options, please contact us for a custom quote.

Check out our Cas9 Stable Cell Lines!

Buy	Catalog#	Description	Number of target sites	Selection marker	Reporter Gene	Vector
Non-viral clones
	CP-C9NU-01	Cas9 nuclease expression clone (Purified plasmid)	1	Neo	mCherry	CP-C9NU-01
	HCP296965-SG01-1	1 × sgRNA expression clone targeting NEDD4L (NM_015277.5)	1	N/A	Hygro	pCRISPR-SG01
	HCP296965-SG01-3	3 × sgRNA expression clone targeting NEDD4L (NM_015277.5)	3	N/A	Hygro	pCRISPR-SG01
Lentivral clones (packaged particles can be found under Lentiviral particle tab)
	CP-LvC9NU-01	Cas9 nuclease lentiviral expression clone (Purified plasmid)	1	Neo	N/A	CP-LvC9NU-01
	CP-LvC9NU-02	Cas9 nuclease lentiviral expression clone (-CMV promoter) (Purified plasmid)	1	Neo	eGFP	CP-LvC9NU-02
	CP-LvC9NU-08	Cas9 nuclease lentiviral expression clone (Purified plasmid)	1	Puro	N/A	CP-LvC9NU-08
	CP-LvC9NU-09	Cas9 nuclease lentiviral expression clone (-EF1a promoter) (Purified plasmid)	1	Neo	eGFP	CP-LvC9NU-09
	CP-LvC9NU-10	Cas9 nuclease lentiviral expression clone (Purified plasmid)	1	Hygro	eGFP	CP-LvC9NU-10
	HCP296965-LvSG03-1	1 × sgRNA lentiviral expression clone targeting NEDD4L (NM_015277.5)	1	mCherry	Puro	pCRISPR-LvSG03
	HCP296965-LvSG03-3	3 × sgRNA lentiviral expression clone targeting NEDD4L (NM_015277.5)	3	mCherry	Puro	pCRISPR-LvSG03
All-in-one clones
	HCP296965-CG02-1	1 × sgRNA/Cas9 all-in-one expression clone targeting NEDD4L (NM_015277.5)	1	N/A	N/A	pCRISPR-CG02
	HCP296965-CG02-3	3 × sgRNA/Cas9 all-in-one expression clone targeting NEDD4L (NM_015277.5)	3	N/A	N/A	pCRISPR-CG02
	HCP296965-CG04-1	1 × sgRNA/Cas9 all-in-one expression clone targeting NEDD4L (NM_015277.5)	1	Neo	copGFP	pCRISPR-CG04
	HCP296965-CG04-3	3 × sgRNA/Cas9 all-in-one expression clone targeting NEDD4L (NM_015277.5)	3	Neo	copGFP	pCRISPR-CG04
	HCP296965-CG07-1	1 × sgRNA/Cas9 all-in-one expression clone targeting NEDD4L (NM_015277.5)	1	N/A	copGFP	pCRISPR-CG07
	HCP296965-CG07-3	3 × sgRNA/Cas9 all-in-one expression clone targeting NEDD4L (NM_015277.5)	3	N/A	copGFP	pCRISPR-CG07
	HCP296965-CG08-1	1 × sgRNA/Cas9 all-in-one expression clone targeting NEDD4L (NM_015277.5)	1	N/A	mCherry	pCRISPR-CG08
	HCP296965-CG08-3	3 × sgRNA/Cas9 all-in-one expression clone targeting NEDD4L (NM_015277.5)	3	N/A	mCherry	pCRISPR-CG08
	HCP296965-CG12-1	1 × sgRNA/Cas9 all-in-one expression clone targeting NEDD4L (NM_015277.5)	1	Neo	mCherry	pCRISPR-CG12
	HCP296965-CG12-3	3 × sgRNA/Cas9 all-in-one expression clone targeting NEDD4L (NM_015277.5)	3	Neo	mCherry	pCRISPR-CG12
AAV clones
	HCP296965-AD01-1	1 × sgRNA/Cas9 AAV expression clone targeting NEDD4L (NM_015277.5)	1	N/A	N/A	pCRISPR-AD01
	HCP296965-AD01-3	3 × sgRNA/Cas9 AAV expression clone targeting NEDD4L (NM_015277.5)	3	N/A	N/A	pCRISPR-AD01
	HCP296965-AV20-1	1 × sgRNA AAV expression clone targeting NEDD4L (NM_015277.5)	1	N/A	N/A	pCRISPR-AV20
	HCP296965-AV20-3	3 × sgRNA AAV expression clone targeting NEDD4L (NM_015277.5)	3	N/A	N/A	pCRISPR-AV20
	HCP296965-AV22-1	1 × sgRNA AAV expression clone targeting NEDD4L (NM_015277.5)	1	N/A	eGFP	pCRISPR-AV22
	HCP296965-AV22-3	3 × sgRNA AAV expression clone targeting NEDD4L (NM_015277.5)	3	N/A	eGFP	pCRISPR-AV22

For species other than human/mouse or for custom orders, click CRISPR

Warranty Statements

HDR Donor Clones

Homology-dependent repair (HDR) gene knockout donors are designed to knock out your gene of interest using GeneCopoeia CRISPR or TALEN clones. Choose a vector backbone for your gene-specific HDR knockout clone from the list below. For other HDR donor applications, including conditional knockout, mutagenesis, in-frame fusion tagging, and more, please contact us for a custom quote.

Catalog#	Description	Vector	Promoter	Reporter Gene	Selection marker	LoxP site
DC-HTN296965-D01	Genome-Editing donor clone (copGFP/Puromycin) for knockout of NEDD4L (NM_015277.5)	pDonor-D01	EFa1	copGFP	Puromycin	N/A
DC-HTN296965-D02	Genome-Editing donor clone (copGFP/Neomycin) for knockout of NEDD4L (NM_015277.5)	pDonor-D02	CMV	copGFP	Neomycin	N/A
DC-HTN296965-D03	Genome-Editing donor clone (Neomycin) for knockout of NEDD4L (NM_015277.5)	pDonor-D03	CMV	N/A	Neomycin	N/A
DC-HTN296965-D04	Genome-Editing donor clone (Puromycin) for knockout of NEDD4L (NM_015277.5)	pDonor-D04	CMV	N/A	Puromycin	N/A
DC-HTN296965-D05	Genome-Editing donor clone (Neomycin) for knockout of NEDD4L (NM_015277.5)	pDonor-D05	EFa1	N/A	Neomycin	N/A
DC-HTN296965-D07	Genome-Editing donor clone (copGFP/Puromycin/TK/LoxP) for knockout of NEDD4L (NM_015277.5)	pDonor-D07	EFa1	copGFP	Puromycin/TK	LoxP
DC-HTN296965-D08	Genome-Editing donor clone (copGFP/Neomycin/TK/LoxP) for knockout of NEDD4L (NM_015277.5)	pDonor-D08	CMV	copGFP	Neomycin/TK	LoxP
DC-HTN296965-D09	Genome-Editing donor clone (Puromycin/TK/LoxP) for knockout of NEDD4L (NM_015277.5)	pDonor-D09	EFa1	N/A	Puromycin/TK	LoxP
DC-HTN296965-D10	Genome-Editing donor clone (Neomycin/TK/LoxP) for knockout of NEDD4L (NM_015277.5)	pDonor-D10	CMV	N/A	Neomycin/TK	LoxP
DC-HTN296965-D11	Genome-Editing donor clone (PGK/copGFP/Puro/TK/LoxP) for knockout of NEDD4L (NM_015277.5)	pDonor-D11	PGK	copGFP	Puromycin	LoxP
DC-HTN296965-D12	Genome-Editing donor clone (EF1a/copGFP/Hygro/TK/LoxP) for knockout of NEDD4L (NM_015277.5)	pDonor-D12	EFa1	copGFP	Hygromycin/TK	LoxP
DC-HTN296965-D13	Genome-Editing donor clone (PGK/copGFP/Neo/TK/LoxP) for knockout of NEDD4L (NM_015277.5)	pDonor-D13	PGK	copGFP	Neomycin/TK	LoxP
DC-HTN296965-D14	Genome-Editing donor clone (PGK/Puro/TK/LoxP) for knockout of NEDD4L (NM_015277.5)	pDonor-D14	PGK	N/A	Puromycin/TK	LoxP

Contact us for custom-designed donor clones for applications such as mutagenesis, tagging, conditional knockout and more. Contact us for custom-designed donor clones for applications such as mutagenesis, tagging, conditional knockout and more. Contact us for custom-designed donor clones for applications such as mutagenesis, tagging, conditional knockout and more.

HDR Donor Vectors

Vector	Description	Promoter	Reporter Gene	Selection marker	LoxP site
pDonor-D01	Genome-Editing Donor cloning vector (copGFP/Puromycin)	EFa1	copGFP	Puromycin	N/A
pDonor-D02	Genome-Editing Donor cloning vector (copGFP/Neomycin)	CMV	copGFP	Neomycin	N/A
pDonor-D03	Genome-Editing Donor cloning vector (Neomycin)	CMV	N/A	Neomycin	N/A
pDonor-D04	Genome-Editing Donor cloning vector (Puromycin)	CMV	N/A	Puromycin	N/A
pDonor-D05	Genome-Editing Donor cloning vector (Neomycin)	EFa1	N/A	Neomycin	N/A
pDonor-D07	Genome-Editing Donor cloning vector (copGFP/Puromycin/TK/LoxP)	EFa1	copGFP	Puromycin/TK	LoxP
pDonor-D08	Genome-Editing Donor cloning vector (copGFP/Neomycin/TK/LoxP)	CMV	copGFP	Neomycin/TK	LoxP
pDonor-D09	Genome-Editing Donor cloning vector (Puromycin/TK/LoxP)	EFa1	N/A	Puromycin/TK	LoxP
pDonor-D10	Genome-Editing Donor cloning vector (Neomycin/TK/LoxP)	CMV	N/A	Neomycin/TK	LoxP
pDonor-D11	Genome-Editing Donor cloning vector (copGFP/Puromycin/LoxP)	PGK	copGFP	Puromycin	LoxP
pDonor-D12	Genome-Editing Donor cloning vector (copGFP/Hygromycin/TK/LoxP)	EFa1	copGFP	Hygromycin/TK	LoxP
pDonor-D13	Genome-Editing Donor cloning vector (copGFP/Neomycin/TK/LoxP)	PGK	copGFP	Neomycin/TK	LoxP
pDonor-D14	Genome-Editing Donor cloning vector (Puromycin/TK/LoxP)	PGK	N/A	Puromycin/TK	LoxP

Contact us for custom-designed donor clones for applications such as mutagenesis, tagging, conditional knockout and more.

IndelCheck™ CRISPR/TALEN insertion or deletion (indel) detection system (version 2.0)

Catalog#	Product	Description	Price
IC001	IndelCheck™ CRISPR/TALEN insertion or deletion detection system	Includes target site PCR kit (IC003) and T7 endonuclease I assay kit (IC005)	inquire
IC002	IndelCheck™ CRISPR/TALEN insertion or deletion detection system	Includes target site PCR kit (IC004) and T7 endonuclease I assay kit (IC006)	inquire
IC003	Target site PCR Kit (version 2.0)	PCR reagents for amplifying region flanking CRISPR/TALEN target site, prior to T7 Endonuclease I digestion*	inquire
IC004	Target site PCR Kit (version 2.0)	PCR reagents for amplifying region flanking CRISPR/TALEN target site, prior to T7 Endonuclease I digestion*	inquire
IC005	T7 endonuclease I assay kit	Cleave mismatched PCR products using T7 endonuclease I to detect indel mutations	inquire
IC006	T7 endonuclease I assay kit	Cleave mismatched PCR products using T7 endonuclease I to detect indel mutations	inquire
IC007	Smart-Join™ Target Site PCR Cloning Kit	Smart-Join™ Target Site PCR Cloning Kit, 20 reaction	inquire
IC008	Smart-Join™ Target Site PCR Cloning Kit	Smart-Join™ Target Site PCR Cloning Kit, 100 reaction	inquire

Target PCR primer pair

Buy	Catalog#	Product	Target site
Target site PCR primer (designed to only amplify the genomic DNA region including the corresponding genecopoeia CRISPR target site(s))
	PHCP296965-a	Target site PCR primer pair for indel detection (target site 1)(200 rxn)	Target site 1
	PHCP296965-b	Target site PCR primer pair for indel detection (target site 2)(200 rxn)	Target site 2
	PHCP296965-c	Target site PCR primer pair for indel detection (target site 3)(200 rxn)	Target site 3
Target site PCR primer for nickase pair (designed to only amplify the genomic DNA region including the corresponding genecopoeia CRISPR target site(s))
	PHCP296965-2N	Target site PCR primer for indel detection (nickase pair target site)(200 rxn)	Nickase pair target site

Chromosomal validation

Buy	Catalog#	Product	Target site
CRISPR Chromosomal validation service
	C-HCP296965-a	Chromosomal validation service (target site 1)	Target site 1
	C-HCP296965-b	Chromosomal validation service (target site 2)	Target site 2
	C-HCP296965-c	Chromosomal validation service (target site 3)	Target site 3
TALEN Chromosomal validation service
	C-HTN296965-a	Chromosomal validation service (target site 1)	Target site 1
	C-HTN296965-b	Chromosomal validation service (target site 2)	Target site 2
	C-HTN296965-c	Chromosomal validation service (target site 3)	Target site 3

Additional services

Description	Price
Donor service	Inquire
Stable cell line service	Inquire
Transgenic mouse service	Inquire
In vitro transcription service	Inquire

Description	Price
Donor service	Inquire
Stable cell line service	Inquire
Transgenic mouse service	Inquire
In vitro transcription service	Inquire
Chromosomal level validation service	Inquire

Description	Price
Donor service	Inquire
Stable cell line service	Inquire
Transgenic mouse service	Inquire
In vitro transcription service	Inquire
Chromosomal level validation service	Inquire

Safe Harbor Knockin

Buy	Catalog#	Product	Description	Price
Human AAVS1 safe harbor knockin
	SH029	Genome-TALER™ human AAVS1 safe harbor gene knock-in kit	Includes: AAVS1 TALEN pair (TN-AAVS1) AAVS1 donor cloning vector (DC-DON-SH01) AAVS1 positive control donor (DC-RFP-SH01) knock-in verification primer pairs (HQPAVSHR)	inquire
	SH025	Genome-TALER™ human AAVS1 safe harbor gene knock-in kit (without donor)	Includes: AAVS1 TALEN pair (TN-AAVS1) AAVS1 positive control (DC-RFP-SH01) knock-in verification primer pairs (HQPAVSHR)	inquire
	SH004	Genome-CRISP™ human AAVS1 safe harbor gene knock-in kit	Includes: AAVS1 All-in-one sgRNA/Cas9 expression clone (HCP-AAVS1-CG02) AAVS1 donor cloning vector (DC-DON-SH01) AAVS1 positive control donor (DC-RFP-SH01) knock-in verification primer pairs (HQPAVSHR)	inquire
	SH000	Genome-CRISP™ human AAVS1 safe harbor gene knock-in kit (without donor)	Includes: AAVS1 All-in-one sgRNA/Cas9 expression clone (HCP-AAVS1-CG02) AAVS1 positive control donor (DC-RFP-SH01) knock-in verification primer pairs (HQPAVSHR)	inquire
Mouse ROSA26 safe harbor knockin
	SH079	Genome-TALER™ mouse ROSA26 safe harbor gene knock-in kit	Includes: ROSA26 TALEN pair (TN-ROSA26) ROSA26 donor cloning vector (DC-DON-SH02) ROSA26 positive control donor (DC-RFP-SH02) knock-in verification primer pairs (MQPROSHR)	inquire
	SH075	Genome-TALER™ mouse ROSA26 safe harbor gene knock-in kit (without donor)	Includes: ROSA26 TALEN pair (TN-ROSA26) ROSA26 positive control (DC-RFP-SH02) knock-in verification primer pairs (MQPROSHR)	inquire
	SH054	Genome-CRISP™ mouse ROSA26 safe harbor gene knock-in kit	Includes: ROSA26 sgRNA/Cas9 expression clone (MCP-ROSA26-CG01) ROSA26 donor cloning vector (DC-DON-SH02) ROSA26 positive control donor (DC-RFP-SH02) knock-in verification primer pairs (MQPROSHR)	inquire
	SH050	Genome-CRISP™ mouse ROSA26 safe harbor gene knock-in kit (without donor)	Includes: ROSA26 sgRNA/Cas9 expression clone (MCP-ROSA26-CG01) ROSA26 positive control (DC-RFP-SH02) knock-in verification primer pairs (MQPROSHR)	inquire
Cas9 safe harbor knockin clones
	SH300	human AAVS1 safe harbor Cas9 knock-in clone	AAVS1 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into human AAVS1 safe harbor locus.	inquire
	SH301	human AAVS1 safe harbor Cas9 knock-in clone	AAVS1 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into human AAVS1 safe harbor locus. (Hygromycin)	inquire
	SH302	human AAVS1 safe harbor Cas9 knock-in clone	AAVS1 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into human AAVS1 safe harbor locus. (Neomycin)	inquire
	SH350	mouse ROSA26 safe harbor Cas9 knock-in clone	ROSA26 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into mouse ROSA26 safe harbor locus. (Puromycin)	inquire
	SH351	mouse ROSA26 safe harbor Cas9 knock-in clone	ROSA26 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into mouse ROSA26 safe harbor locus. (Hygromycin)	inquire
	SH352	mouse ROSA26 safe harbor Cas9 knock-in clone	ROSA26 knock-in ORF clone containing CBh-driven Cas9 nuclease gene. For integrating Cas9 gene into mouse ROSA26 safe harbor locus. (Neomycin)	inquire
	Variable	AAVS1 knock-in ORF donor clone	AAVS1 knock-in ORF donor clone containing CMV-driven ORF of customer's gene of interest

Related products

For TALEN/CRISPR-mediated human and mouse safe harbor site knockin, GeneCopoeia offers Safe Harbor knockin kits and Safe Harbor knockin human ORF clones.