Using a secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ promoter clones are designed for promoter analysis by detecting the real-time activities of over 20,000 human and 18,000 mouse promoters using live cell assays. GLuc-ON™ promoter clones can be ordered as pre-designed or custom-built. We offer Gaussia-luciferase (GLuc), eGFP, or mCherry based lentiviral and non-viral promoter reporter clones and assay kits, which are powerful tools to study transcription regulation. Gaussia luciferase is a secreted luciferase and is 1,000-fold more sensitive than firefly or Renilla luciferase.
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How Secreted Reporters Work |
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Publication highlights
- Schank, JR. et al (2013) Tacr1 Gene Variation and Neurokinin1 Receptor Expression Is Associated with Antagonist Efficacy in Genetically Selected Alcohol-Preferring Rats. Biological Psychiatry Journal, PII: S0006-3223(13)00043-7, doi:10.1016/j.biopsych.2012.12.027. [custom Tacr1 promoter luciferase construct].
- Mills, L.D. et al. (2013) Loss of the Transcription Factor GLI1 Identifies a Signaling Network in the Tumor Microenvironment Mediating KRAS-Induced Journal of Biological Chemistry, March 12, 2013, doi: 10.1074/jbc.M112.438846. [mouse SHH promoter clone].
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