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Validated All-in-One™ qPCR Primer for UHRF1(NM_001290051.2) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Summary
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene References into function
- ICBP90 expression altered in cancer cell lines and upregulated by E2F-1 overexpression, with efficiency depending on cancer status of cell line
- Apoptosis is dependent upon ICBP90 expression downregulation and ICBP90 exhibits anti-apoptotic properties.
- ICBP90 is involved in cell proliferation by way of methylation-mediated regulation of certain genes.
- ICBP90 down-regulation is a key event in G1 arrest preceding T cell death
- ICBP90 overexpression is involved in the altered checkpoint controls occurring in cancerogenesis
- General requirement for UHRF1 in tumor cell proliferation implicates the RING domain of UHRF1 as a functional determinant of growth regulation.
- These findings collectively indicate that the human NP95 gene is the functional orthologue of the murine Np95 gene.
- data suggest that UHRF1 may help recruit DNMT1 to hemimethylated DNA to facilitate faithful maintenance of DNA methylation
- A new role of ICBP90 in the relationship between histone ubiquitination and DNA methylation in the context of tumoral angiogenesis and tumour suppressor genes silencing.
- ICBP90 is required for proper heterochromatin formation in mammalian cells
- Parasite proliferation was suppressed in G1-arrested cells induced by UHRF1-siRNA, indicating the importance of the G2 phase via UHRF1-induced G1/S transition for T. gondii growth.
- the 1.7 A crystal structure of the apo SRA domain of human UHRF1 and a 2.2 A structure of its complex with hemi-methylated DNA, revealing a previously unknown reading mechanism for methylated CpG sites (mCpG)
- structural analysis and hemimethylated CpG binding of the SRA domain from human UHRF1
- UHRF1 recruited and cooperated with G9a to inhibit the p21 promoter activity, which correlated with the elevated p21 protein level in human UHRF1 siRNA-transfected HeLa cells.