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Validated All-in-One™ qPCR Primer for DICER1(NM_177438.3) Search again
Product ID:
HQP171499
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD
Gene Description:
dicer 1, ribonuclease III
Target Gene Accession:
NM_177438.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Summary
This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene.
Gene References into function
- cloning and expression of the 218 kDa human Dicer, and characterization of its ribonuclease activity and dsRNA-binding properties
- purification and properties of a recombinant human Dicer
- fragile X syndrome CGG repeats readily form RNA hairpins and is digested by the human Dicer enzyme, a step central to the RNA interference effect on gene expression
- Dicer has a single RNA post-transcriptional processing center
- Dicer is essential for formation of the heterochromatin structure in vertebrate cells.
- various attributes of the 3' end structure, including overhang length and sequence composition, play a primary role in determining the position of Dicer cleavage in both dsRNA and unimolecular, short hairpin RNA
- RNA containing the AU-rich element of GM-CSF is destabilized by dicer and positive charge of proteins
- The C-terminal RNase III domain (RNase IIIb) of human Dicer was expressed, purified and crystallized by the sitting-drop vapour-diffusion method
- Dicer up-regulation may explain an almost global increase of microRNA expression in prostate adenocarcinoma.
- Up-regulation of TSSC3 occurred in Dicer knockdown cells.
- Transcripts containing long hairpin structures composed of CNG repeats are another class of Dicer targets.
- Overexpression of Dicer correlates with precancerous conditions for Lung adenocarcinoma.
- These results suggest that, in the context of HIV replication, TRBP contributes mainly to the enhancement of virus production and that Dicer does not mediate HIV restriction by RNAi.
- Maintenance and regulation of endogenous microRNA levels via Dicer-mediated processing is critical for endothelial cell gene expression and functions in vitro.
- Results indicate that human TRBP and PACT directly interact with each other and associate with Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA.
- a unique amino acid sequence in human DICER protein is essential for binding to Argonaute family proteins
- Results show that recombinant Dicer is capable of cleaving the TAR element in vitro and that TAR derived miRNA is present in HIV-1 infected cell lines and primary T-cell blasts.
- a role for the PAZ domain of Dicer in binding ssRNAs.
- DICER1 may play an important role in the development of cancer and the epigenetical regulation involved.
- Ago2, Dicer, and TRBP comprise the RISC-loading complex (RLC) and assembles spontaneously in vitro from purified components
- Abnormal immunoexpression of Dicer in aggressive mucoepidermoid carcinoma suggests a role for microRNA and microRNA machinery in tumor progression.
- the role of Dicer, one of the central proteins of the miRNA processing machinery during apoptosis, and show that down-regulation of Dicer results in accelerated apoptosis of HeLa cells, triggered by TNFalpha (tumour necrosis factor alpha).
- Our results suggest that HCV core protein may abrogate host cell RNA silencing defense by suppressing the ability of Dicer to process precursor dsRNAs into siRNAs.
- Intact DICER is required to maintain full promoter DNA hypermethylation of select epigenetically silenced loci in human cancer cells.
- These results suggest that the DExD/H-box domain likely disrupts the functionality of the Dicer active site until a structural rearrangement occurs, perhaps upon assembly with its molecular partners.
- Chemical modifications patterns compatible with high potency dicer-substrate small interfering RNAs are reported.
- Up-regulation of MICA and MICB is the result of DNA damage response activation caused by Dicer knockdown. RNAi is indirectly linked to the human innate immune system via the DNA damage pathway.
- luciferase assay using a reporter carrying a putative target site in the 3' untranslated region of Dicer revealed that let-7 directly affects Dicer expression
- Evidence that a Dicer helicase mutant is sensitive to the thermodynamic properties of the stems in microRNAs and short-hairpin RNAs, with thermodynamically unstable stems resulting in poor processing and a reduction in the levels of functional mi/siRNAs.
- Downregulation of Dicer expression by serum withdrawal sensitizes human endothelial cells to apoptosis.
- Dicer cleaved substrates containing short siRNA-like double-strand regions and extended 3' or 5' ssRNA overhangs in the adjacent ssRNA regions
- knockdown of Dicer inhibits human breast carcinoma cell growth
- Our findings indicate that levels of Dicer and Drosha mRNA in ovarian-cancer cells have associations with outcomes in patients with ovarian cancer.
- Data demonstrated a direct interaction between Dicer and Wig-1 and both may play a common role in dsRNA-related gene regulation.
- Phosphorylation of FMRP regulates its association with the miRNA pathway by modulating association with Dicer.