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Validated All-in-One™ qPCR Primer for SPP1(NM_001251830.2) Search again
Product ID:
HQP161272
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BNSP, BSPI, ETA-1, OPN
Gene Description:
secreted phosphoprotein 1
Target Gene Accession:
NM_001251830.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Gene References into function
- upregulated transcripts in brain of patients with multiple sclerosis
- mapping of functional epitopes that are important to cell adhesion and migration
- Increased expression is associated with breast tumor metastasis
- The osteopontin gene, which has a role in immunosurveillance, is a direct target of TP53.
- Three SIBLINGs (small integrin-binding ligand, N-linked glycoproteins) enhance factor H's cofactor activity enabling MCP-like cellular evasion of complement-mediated attack.
- OPN induced a profound level of synthesis of IL-12 from noninfected PBMCs. The major cellular source of OPN was monocytes.
- PPARgamma ligands inhibit OPN gene expression through the interference with the binding of nuclear factors to A/T-rich sequence in THP-1 cells.
- an association between levels of a biomarker, osteopontin, and ovarian cancer suggest its clinical usefulness
- Osteopontin modulates prostate carcinoma invasive capacity through RGD-dependent upregulation of plasminogen activators.
- A silent polymorphism (707C>T, rs1126616) of osteopontin was significantly associated with systemic lupus erythematosus
- Osteopontin was suggested to play a role in HCC, especially in cancer-stromal interactions.
- RT-PCR analysis of human bone marrow stromal cells during osteogenesis in vitro: the mRNA levels of bone morphogenetic protein-2 (BMP-2), bone sialoprotein-II (BSP), osteopontin (OP) and cbfa-1 increased with culture time in osteogenic medium.
- has RGD sequence and role in osteoclast adhesion
- Results provide the first evidence that osteopontin may play an important role in stone formation in chronic pancreatitis.
- in human tumor cells, HGF and M-CSF stimulate osteopontin production (which is subsequently used as a substrate for cell adhesion)
- crystal retention in the human kidney may depend on the expression of CD44-, OPN-, and-HA rich cell coats by damaged distal tubular epithelium.
- OPN-induced migration of mammary epithelial cells also requires activation of the EGF pathway. EGF & OPN synergistically induced cell migration. Exogenous OPN increased ligand (TGFalpha> EGF) & EGFR mRNA expression, as well as EGFR kinase activity.
- Osteopontin, which was identified as a lead gene in the signature, was over-expressed in metastatic HCC
- Elevated osteopontin (OPN) transcription often correlates with increased metastatic potential of transformed cells, and in several model systems OPN--whether produced by the tumor cells or by stromal cells - has been shown to enhance metastatic ability.
- osteopontin as the leading candidate clinical marker derived from a screen of approximately 12,000 named genes.
- Association with disease course is detectable in patients with at least one wild-type OPN 1284A allele: these patients are less likely to have a mild disease course and are at increased risk for a secondary-progressive clinical type.
- first report that osteopontin induces nuclear factor kappaB activity and urokinase secretion by activating phosphatidylinositol 3'-kinase/Akt protein/Ikappab kinase-mediated signaling pathways
- study indicates the possibility that osteopontin plays an important role in the pathogenesis of ulcerative colitis via increased immune activity
- OPN expression is significantly higher in malignant epithelial sources over normal and benign epithelial sources, but no difference in expression levels is evident between primary tumors with or without metastases.
- In patients with previous anterior wall myocardial infarction(MI) osteopontin is released from heart into coronary circulation in proportion to left ventricular(LV) systolic function and volumes. Osteopontin may be associated with post-MI LV remodeling.
- OPN binds to the alphavbeta3-integrin to increase plasma membrane CD44v6 expression and augment in vitro adhesion to hyaluronate
- increased expression of the alpha(v)beta(3) integrin during breast cancer progression can make tumor cells more responsive to malignancy-promoting ligands such as OPN and result in increased tumor cell aggressiveness.
- OPN is expressed in breast and prostate cancer and has a role in differentiation and activation of osteoclasts
- platelet adhesion to osteopontin-coated surfaces requires an agonist-induced exposure of alpha v beta 3-binding sites for this ligand
- Opn is among the most abundantly expressed proteins in a range of lung diseases and has been shown to regulate aspects of pulmonary granuloma formation, fibrosis, and malignancy (review)
- Subjects carrying haplotype B and/or C had an 8-fold higher risk of developing Dianzani autoimmune/lymphoproliferative disease than haplotype A homozygotes.
- induces alpha(v)beta(3) integrin-mediated AP-1 activity and urokinase type plasminogen activator secretion by activating c-Src/EGFR/ERK signaling pathways
- OPN promoter region SNP at nt -433 may be a useful marker reflecting hepatitis activity in chronic hepatitis C patients.
- Epidermal growth factor may regulate osteopontin gene expression through PI3K signaling pathway in hepatocellular carcinoma cells
- Nurr1 activates the OPN promoter directly in osteoblastic cells and may have a role in the regulation of bone homeostasis
- Beta-catenin, Lef-1, Ets transcription factors, and the AP-1 protein c-Jun each weakly enhanced luciferase expression from an OPN promoter.
- Notch1 gene expression was decreased 5-fold in osteopontin-treated CD34+ cells
- Myeloma cell lines adhere to OPN, so high stromal OPN expression may be partly responsible for retention of myeloma cells in bone marrow. The high plasma OPN levels may be due to OPN production by tumor cells & tumor-induced production by non-tumor cells
- The large increase in OPN expression in tumors compared with normal tissue and its association with survival suggest a role for OPN in lung tumorigenesis.
- Osteopontin is expressed at high concentrations in secretory endometrium in epithelial cells and in decidua in stromal cells. Decidual stromal expression of osteopontin is up-regulated by decidualization.
- Decreased expression of OPN is considered to be a reliable indicator of tumor aggressiveness and clinical outcome in patients with intrahepatic cholangiocarcinoma.
- Data demonstrate that early down-regulation of osteopontin in vitro inhibits human osteoclastogenesis.
- osteopontin promoter polymorphisms affect its transcriptional activity
- Review. OPN's role in bone and its role in adhesion, migration, cell survival, and tumorigenesis.
- Molecular structure of OPN, evidence for its functional role in tumor cell metastasis, downstream signals that activate invasive mechanisms, and recent reports concerning regulation of OPN transcription [review]
- analysis of osteopontin peptides and their roles in cell adhesion
- highly phosphorylated human OPN-1 can stimulate trophoblastic cell migration and provides evidence for the involvement of the PI3K/mTOR/p70 S6 kinase pathway in the JAR cells response
- OPN was higher in proliferated bile ductules of acute-form fulminant hepatitis than in cirrhotic and normal liver bile ducts. OPN in proliferated bile ductules was decreased in subacute form of fulminant hepatitis.
- Rotavirus infection of intestinal epithelial cells caused marked increases in OPN mRNA levels and secreted OPN protein
- Data show that mRNA expression of osteopontin (OPN) was elevated in CD4(+) synovial T cells derived from patients with rheumatoid arthritis, which correlated with increased OPN concentrations in synovial fluid (SF).
- Even though the role of osteopontin in the cytopathogenesis of atypical teratoid/rhabdoid tumor still needs to be determined, data support that overexpressed osteopontin is a potential diagnostic marker for atypical teratoid/rhabdoid tumor.
- up-regulation of OPN expression by interlobular biliary epithelium in biliary atresia, which correlates with biliary proliferation and portal fibrosis
- identify OPN as an important tissue-derived factor that DCs encounter when traveling from peripheral sites of activation to secondary lymphatic organs, which induces DC maturation toward a Th1-promoting phenotype
- ability of Bcr-Abl to stimulate the expression of OPN
- Circulating levels of osteopontin, but not IFN-gamma or IL-12, reflect disease activity in patients with tuberculosis.
- SNPs in the promoter region of the osteopontin gene as a marker predicting the efficacy of interferon-based therapies in patients with chronic hepatitis C.
- Osteopontin (OPN) genotypes may influence multiple sclerosis development and progression due to their influence on OPN levels.
- OPN has a role in foreign body giant cell formation in response to implantation
- osteopontin is one of the crystal-associated urine proteins involved in the formation of the organic layers of the plaque particles in kidney stones
- OPN and CEACAM1 may act as a functional complex involved in the regulation of placental invasiveness.
- Increase in osteopontin is associated with pancreatic cancers and chronic pancreatitis
- Overall these results show that OPN is deregulated by BCR-ABL oncogene and suggest that OPN could be involved in CML stem cell biology.
- Granular cell tumor among Schwannian cell tumors consistently express OPN, but its biological significance requires further investigation.
- CD44 and/or OPN may be molecular targets for therapeutic intervention in aggressive papillary thyroid carcinomas.
- Osteopontin overexpression is common in primary non-small cell lung cancer (NSCLC) and may be important in the cancer development and progression and osteopontin plasma levels may serve as a biomarker for diagnosing or monitoring patients with NSCLC.
- integrin alphavbeta6, alphavbeta3, alphavbeta5, alpha5beta1 and alpha9beta1 binding to osteopontin is controlled by specific structural motifs that are recognized by extracellular proteases
- Three promoter polymorphisms of the human Eta-1 gene, -443T/C, -156delG/G, -66T/G, were investigated for possible influence on gene expression.
- These data suggest that nitric oxide enhances hepatocellular cancer expression of OPN and, as a result, conveys a metastatic phenotype.
- SPP1 mRNA increases during acute lung injury in mice
- localizes with the follicular dendritic cell (FDC) network in lymphatic tissues in HIV-1 infection and has a role in lymphoid follicle pathology and repair and B cell regulation
- Polymorphism at position 9,402 of the OPN gene is highly associated with urinary calcium stones, whereas polymorphism at position 9,171 of the OPN gene is not associated with urinary calcium stone disease.
- Results support the hypothesis of an association between high osteopontin expression and poor survival of patients with stage I non-small cell lung cancer, suggesting that osteopontin could be a candidate target for cancer therapy.
- TIS7, a negative regulator of transcriptional activity, represses expression of OPN and beta-catenin/Tcf-4 target genes
- silencing Runx2 with siRNA in myeloma cells suppressed OPN mRNA and protein expression, critical for the proangiogenic effect of myeloma cells
- Results identify osteopontin as a marker and a potential therapeutic target in cases of aggressive laryngeal squamous cell carcinomas.
- increased OPN level may be involved in the malignant transformation of prostate epithelial cells and OPN expression level is an important determinant for patient survival
- high plasma levels of OPN in patients with a history of percutaneous coronary intervention were associated with the presence of restenosis, suggesting that OPN may play a role in the development of restenosis after percutaneous coronary intervention
- osteopontin contributes to several steps in the process of prostate carcinogenesis and metastasis
- Elevated osteopontin in urine is associated with ovarian cancer
- Results suggest osteopontin is dependent on ERalpha for its expression.
- High osteopontin expression is associated with non-small cell lung cancers
- OPN acts downstream of PI3K in melanoma and PTEN loss contributes to melanoma development
- Protein kinase B (Akt) gene regulated osteopontin expression at the RNA level and protein level.
- has a pivotal role in the development of immune responses and in the tissue destruction and the subsequent repair processes associated with inflammatory diseases
- study shows the expression pattern of osteopontin in cellular populations of normal endometrium and in endometrial carcinoma and its correlation with the expression of CEACAM1
- results suggest that OPN might suppress multiplication of Plasmodium falciparum through T helper 1 cells-mediated immune responses
- High Osteopontin is associated with hepatocellular carcinoma
- Real-time RT-PCR confirmed up-regulation of IL-8, osteopontin, and TNFRSF14 and down-regulation of SAMeS and CD209 in AH.
- OPN-transfected cells express high levels of hyaluronan synthase 2, which is associated with increased HA production and matrix retention and is necessary for tumor cell adhesion to bone marrow endothelial cells and anchorage-independent growth
- hyperphosphatemia or azotemia is associated with the expression of osteopontin in vascular smooth muscle cells in patients with ESRD
- We found that the expression of OPN corroborates with the aggressive phenotype of the breast cancer cells i.e. the expression of OPN is acquired as the breast cancer cells become more aggressive.
- The evaluation of Osteopontin expression is useful for predicting the malignant properties of esophageal squamous cell carcinoma.
- The presence of OPN in the normal human and non-human primate SN coupled to its decreased expression following nigral cell degeneration suggests that it may play an important role in dopaminergic neurone survival.
- Intranuclear OPN participates in the process of cell duplication and associates with Plk1.
- Expression of SPP1 in osteosarcoma is associated with an inflammatory response and bone remodeling, making it a good biomarker.
- OPN is produced in osteolytic lesions: MM-derived OPN plays a critical role in bone disease by protecting bone from destruction.
- Increased blood levels in smokers with type 2 diabetes.
- Review. Osteopontin plays a role in Th1 and IgE-mediated immune responses.
- OPN is involved in Parkinson disease-associated neurodegeneration
- Data suggest that transglutaminase 2 interactions with osteopontin lead to protein polymerization that facilitates macrophage adhesion to the calcified elastic lamellae to promote clearance of the ectopic mineral deposits.
- Osteopontin a tumor-metastasis activator, a crucial downstream target of Breast cancer metastasis suppressor 1 is identified.
- The plasma concentrations of OPN are elevated in patients with ulcerative colitis and that OPN expression is correlated with clinical activity.
- Osteoclast-derived osteopontin and VEGF from myeloma cells cooperatively enhance angiogenesis and also induce osteoclastogenic activity by vascular endothelial cells
- The immunocytochemical localization of OPN in the cytoplasm of pyramidal neurons. In AD brains, there was a significant 41 % increase in the expression of neuron OPN compared with age-matched control brain.
- OPN-CD44(V) interaction promotes ECM-derived survival signal mediated through integrin activation, which may play an important role in the pathogenic development and progression of gastric cancer.
- A possible role of OPN and its receptor(s)is mediating downstream signals in the progression of prostate cancer cells. OPN over expression increases the interaction of CD44 and MMP-9 on the cell surface.
- Treatment of human macrophages with the PPARalpha ligands bezafibrate or WY14643 inhibited OPN expression and transcription.
- demonstrated the role of OPN in two distinct aspects of macrophage accumulation: prevention from recirculation and protection from apoptosis
- Thus, erythrocytes may be specialized cells for storing and supplying plasma S1P.
- that the 795 polymorphism does not play an etiological role per se in multiple sclerosis
- OPN-stimulated IL-10 production increases less in T-cell cultures from Crohn's disease (CD) patients than from healthy controls, indicating that IL-10 deficiency may be involved in the defective immune-regulation in CD.
- Increased expression of an osteopontin splice variant was associated with metastatic hepatocellular carcinoma
- These data confirm the positive role of OPN in gastric-cancer-associated angiogenesis.
- Osteopontin was found more prominently in glandular epithelium than in interstitial space of the endometrium.
- OPN plays a crucial role for tumor growth and angiogenesis of human lung cancer cells in vivo by interacting with alphavbeta3 integrin.
- Frequent osteopontin expression is typical of, but not specific for malignant pleural mesothelioma, whereas it appears to select pulmonary adenocarcinoma cases with p65 and MMP-9 expression, suggesting a link with EGFR signalling pathways.
- Findings suggest that SPP1 polymorphisms might be among the genetic factors for HBV clearance and/or HCC occurrence.
- significant association of two haplotypes located in the OPN promoter with the relative probability of nephrolithiasis; one of increased and one of reduced risk
- Macrophage-derived OPN can compensate for the decrease of OPN and thereby restore the metastatic potential of OPN-knockdown tumor cells.
- the possible mechanisms by which osteopontin may contribute functionally to malignancy, particularly in breast cancer {Review}
- OPN (osteopontin) is a transcriptional target of aberrant Wnt signaling, and OPN expression alone predicts survival in human colon cancer.
- Plasma OPN and mRNA expression of OPN in omental adipose tissue are increased in overweight/obese patients.
- Data indicate that therapeutic targeting of tumor OPN molecules could reset metastatically relevant gene networks, resulting in clinical benefit.
- In SW480 human colon cancer cells, we conclude that Sp1 mediated expression of the tumor metastasis protein, OPN, regulates in vitro functional correlates of tumor metastasis
- OPN is involved in mediating hepatic inflammation and the ensuing liver toxicity --REVIEW
- OPG but not OPN stimulated a dose-dependent increase in the expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin by endothelial cells in the presence of TNF-alpha
- REVIEW of multi-faceted functional role of OPN in breast cancer, in particular the malignancy-promoting aspects of OPN
- ING4 suppression in MM cells up-regulated IL-8 and OPN, increasing the HIF-1alpha activity and its target gene NIP-3
- Reference intervals were established with a high analytic performance for OPN and an acceptable analytic performance for OPG and total sRANKL. The study revealed low biological variation for OPN and total sRANKL and high biological variation for hsCRP.
- Increased expression of osteopontin is associated with gastric carcinoma
- Whole urine from osteopontin, Tamm-Horsfall protein , or double-null mice all possessed a dramatically reduced ability to inhibit the adhesion of calcium oxalate monohydrate crystals to renal epithelial cells
- These data suggest that OPN is associated with keratinocyte differentiation and that it is expressed in AK and SCC, which have metastatic potential, but minimally expressed in solid BCC.
- overexpression of Osteopontin-c is associated with invasive, but not in noninvasive, breast tumor
- Finding identify OPN and CD44v6 as predictive markers of recurrence or aggressiveness in laryngeal intraepithelial neoplasia, and overall, point out an important signalling complex in the evolution of laryngeal dysplasia.
- Osteopontin protein expression was confirmed as a new independent predictor of tumor recurrence and poor prognosis in patients with gastric cancer.
- Osteopontin expression of circulating CD4+ T cells and plasma osteopontin levels reflect the severity of heart failure.
- Osteopontin expression in adipose tissue macrophages was strongly up-regulated by obesity.
- specific recognition of membrane-bound Osteopontin sites on preosteogenic cell membrane
- phosphorylation and glycosylation sites and N-terminal fragments present in urine are generated by proteolytic cleavage
- OPN is required for the differentiation and activity of myofibroblasts formed in response to the profibrotic cytokine transforming growth factor-beta1.
- Osteopontin as well as dystrophy within the tumor may be involved in the stromal calcification of large cell neuroendocrine carcinoma.
- These are the first data to suggest a role for osteocytes and OPN in the recruitment of mesenchymal stem cells to aid in fracture repair.
- syndecan-4 is a critical intrinsic regulator of inflammatory reactions via its effects on OPN function
- Results show that OPN is not valuable as a clinical marker for monitoring the treatment response in multiple myeloma patients because of inconsistency in its levels in multiple myeloma patients.
- Real-time PCR showed that ETOH significantly altered the expression of genes involved in cell adhesion. There was an increase in the expression of alpha and beta Laminins 1, beta Integrins 3 and 5, Secreted phosphoprotein1 and Sarcoglycan epsilon.
- OPN gene 9250 polymorphism appears to be associated with susceptibility to SLE in Chinese Han ethnic population.
- osteopontin (OPN) is highly expressed and secreted by erythroblasts during differentiation
- Demonstrated elevated serum OPN and OPG levels in patients with carotid stenosis and documented an independent association between these biochemical markers, pathology and carotid-induced symptomatology.
- Osteopontin, cyclooxygenase-2 and vascular endothelial growth factor synergically promote angiogenesis and metastasis in gastric cancer.
- production is associated with the inflammatory process of oral lichen planus development
- plasma osteopontin levels are potentially useful as a diagnostic and prognostic biomarker for cervical cancer.
- haplotypes of OPN gene contributed to determining risk factors as well as protective factors of large artery atherosclerosis.
- osteopontin is associated with SLE, and this association is especially stronger in males. To our knowledge, this report serves as the first association of a specific autosomal gene with human male lupus
- Infertile women with isolated polycystic ovaries are deficient in endometrial expression of osteopontin but not alphavbeta3 integrin during the implantation window.
- Osteopontin levels are elevated in patients with peripheral arterial disease.
- osteopontin expression is elevated in ovarian clear cell carcinoma
- Compared with normal pregnancy, osteopontin expression and decidual killer cells accumulation were reduced significantly in recurrent pregnancy loss patients.
- studied gene expression profile of brain lesions of a patient with Neuromyelitis optica by using DNA microarray; found marked up-regulation of interferon gamma-inducible protein 30 (IFI30), CD163, and secreted phosphoprotein 1 (SPP1, osteopontin)
- OPN is a bona fide substrate for thrombin, and generation of thrombin-cleaved OPN with enhanced pro-inflammatory properties provides a molecular link between coagulation and inflammation
- Opn plays an important role in the development of albuminuria, possibly by modulating podocyte signaling and motility
- OPN downregulation by BRMS1 may be responsible, at least in part, for BRMS1-mediated metastasis suppression by sensitizing cancer cells to stress induced apoptosis.
- Osteopontin in the cerebrospinal fluid may be, in part, of central nervous system origin, and may play an important role in central nervous system inflammation.
- osteopontin alternative translation generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells
- The overexpression of osteopontin protein in astrocytoma cells suggests its role in astrocytoma progression and angiogenesis.
- Opn has pivotal role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. [REVIEW]
- Data show that mRNA expression of OPN was significantly increased throughout the window of implantation.
- Novel pathway by which OPN and possibly other Ets-1 target genes involved in tumor metastasis are regulated by TIP30. Mechanism for metastasis promoted by TIP30 deficiency.
- osteopontin:alpha(v)beta(3)integrin complex untenable as marker of endometrial receptivity and implantation potential
- RNA stability is a new, previously unrecognized mechanism that regulates osteopontin expression in hepatocellular carcinoma to convey metastatic function.
- Osteopontin expression was significantly lower in long-term compared with short-term survivors of malignant pleural mesothelioma (P< .0001), and overall survival analysis showed that low osteopontin expression was associated with longer survival.
- osteopontin and IL-8 may have roles in prostate neoplasm recurrence
- This study showed that plasma levels of osteopontin are significantly correlated with HIV-induced CNS dysfunction, and suggested that the development of interventions to modulate osteopontin production or signaling might be beneficial.
- Osteopontin is associated with esophageal tumorigenesis and progression, but not patients' survival.
- Elevated osteopontin serum levels are associated with malignant mesothelioma.
- CST6, CXCL14, DHRS3, and SPP1 are regulated by BRAF signaling and may play a role in papillary thyroid carcinoma pathogenesis
- Results describe the crystal structure of monoclonal antibody 23C3 in complex with its osteopontin epitope peptide.
- Overexpression of osteopontin independently correlates with vascular invasion and thus predicts poor survival for patients with hepatocellular carcinoma.
- Endothelin signaling axis activates osteopontin expression through PI3 kinase pathway in A375 melanoma cells.
- Serum levels of osteopontin are increased in systemic inflammatory response syndrome and sepsis
- analysis of osteopontin expression according to molecular profile of invasive breast cancer
- Osteopontin (OPN) plays an important role in metastasis as well as tumor growth of hepatocellular, in which different minimum threshold levels of OPN are needed.
- Osteopontin combined with CD44 is a promising independent predictor of tumor recurrence and survival in hepatocellular carcinoma patients.
- a significant increase in osteopontin and MMP-9 levels and activity in patients with prostate cancer
- Tumor-associated OPN can serve as an indicator of tumor regrowth.
- OPN promotes ovarian cancer progression and cell survival and increases HIF-1alpha expression through the PI3-K/Akt pathway.
- Analogous to EAE, in subjects with MS, we found increased expression of Opn in DCs and increased expression of the Opn receptors CD44, beta(3), and alpha(v) on T cells.
- High plasma OPN levels are an unfavorable factor for development of cardiovascular disease in patients with psoriasis
- The expression of OPN is upregulated in chronic rhinosinusitis (CRS). The mutual regulatory interactions between OPN and inflammatory cytokines suggest that OPN may play an important role in the pathogenesis of CRS.