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Validated All-in-One™ qPCR Primer for BRD2(NM_001291986.2) Search again
Product ID:
HQP160216
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BRD2-IT1, D6S113E, FSH, FSHRG1, FSRG1, NAT, O27.1.1, RING3, RNF3
Gene Description:
bromodomain containing 2
Target Gene Accession:
NM_001291986.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Summary
This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response.
Gene References into function
- The BRD2 gene is homologous to the mammalian cell cycle regulator TAFII250 and the Drosophila trithorax group gene female sterile homeotic; the gene encodes a nuclear-localized kinase with signal transduction activity.
- Mitogenic stimuli induce nuclear localization of Brd2 protein.
- Brd2 protein participates in a nuclear complex that contains E2Fs. Overexpression of Brd2 protein with oncogenic ras transactivates E2F-dependent promoters of the cell cycle regulatory genes dihydrofolate reductase, cyclin D1, cyclin A and cyclin E.
- The BRD2 gene is located within the human class II major histocompatibility complex at Chromosome 6p21.3, but appears to have no role in antigen processing, and is likely involved in signal transduction and transcription.
- immunoblot and immunoprecipitation experiments to identify proteins interacting with Fsrg1 and RING3
- highly significant linkage disequilibrium between juvenile myoclonic epilepsy and a core haplotype of five single-nucleotide-polymorphism and microsatellite markers in chromosomal region 6p21
- B cell-restricted expression of Brd2 in transgenic mice upregulates cyclin A trancription even in mitogenically unstimulated B cells and causes lymphoma with a B1a-like immunophenotype, accompanied by peripheral B cell leukemia.
- Brd2, a nuclear-localized protein kinase, binds to cyclin A promoter chromatin and recruits histone H4-specific acetylase activity, increasing cyclin A transcription and accelerating S phase entry in fibroblasts.
- Evidence from transgenic mice suggests that Brd2 transactivates the cyclin A locus, and that overexpression increases cyclin A transcription, destabilizing the cell cycle.
- Present results support evidence that photoparoxysmal response and Myoclonic Epilepsy, Juvenile share epileptogenic pathways, for which BRD2 might be an underlying susceptibility gene.
- The results showed that BRD7 could interact with BRD2 and the region from amino acid 430 to 798 of BRD2 was critical for the interaction of BRD2 with BRD7. BRD2 mainly localizes in nucleus and BRD2 has distinct roles in initiating apoptosis.
- there are distinct functional roles for the two bromodomain proteins RING3/Brd2 and Brd4 in LANA binding
- Brd2 is a novel protein kinase that plays a role in cell cycle-responsive transcription, recruiting E2F1, TATA Box Binding Protein and histone acetylase activity to E2F-responsive promoters in response to mitogenic stimuli.
- Brd2 associates with the transcription factor E2F, the Mediator components CDK8 and TRAP220, RNA polymerase II on chromatin; and associates with acetylated histone H4 through its bromodomains.
- Two acetyllysine-binding pockets and a negatively charged secondary binding pocket, produced at the dimer interface in BRD2 bromodomain 1 (BD1), may be the unique features that allow BRD2 BD1 to selectively bind to the acetylated histone H4 tail.
- Brd2-driven B cell lymphomas exhibit a transcriptional signature of diffuse large B cell lymphoma, and show differential expression of genes involved in normal proliferation, as well as several novel, tumor-specific genes.
- Brd2 bromodomain 2 is monomeric in solution and dynamically interacts with H4-AcK12; additional secondary elements in the long ZA loop may be a common characteristic of BET bromodomains.
- The second bromodomain of Brd2 binds histone H4 acetyl-lysine K12.
- study investigated the SNP markers (rs188254, rs206781, and rs516535) in the 3'-end of the BRD2 and found no significant difference between the allele frequencies in epilepsy cases and controls
- Results report that the double bromodomain proteins Brd2 and Brd3 associate preferentially in vivo with hyperacetylated chromatin along the entire lengths of transcribed genes.