|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for POU5F1(NM_002701.6) Search again
Product ID:
HQP159016
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
Gene Description:
POU class 5 homeobox 1
Target Gene Accession:
NM_002701.6(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Gene References into function
- POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors.
- FGF4 is upregulated by the OCT3 transcription factor in breast cancer cells.
- Oct4 can dimerize onto DNA in a distinct conformational arrangement.
- Normal pattern of expression is disturbed in dysgenetic gonads, especially in rare intersex cases, thus increasing risk of malignant transformation. High abundance of OCT-3/4 in carcinoma in situ is consistent with early fetal origin and pluripotency.
- Oct4 is required to maintain the undifferentiated stem cell state, and differentiation to trophectoderm occurs in its absence.
- expression of Oct-4 indicates a pluripotent phenotype of CD133(+) cells and appears to be of functional relevance
- Adult stem cells maintain expression of Oct4, consistent with the stem cell hypothesis of carcinogenesis.
- demonstrated changes in DNA methylation in the promoter regions of Nanog and Oct-4 in a human cell line during retinoic acid-induced differentiation
- The Oct4 is an important marker of the undifferentiated state and a central regulator of pluripotency in ES cells.
- Oct-4 mRNA and protein expression during human preimplantation development was studied.
- Gene knockdown of Oct4 promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
- MAPCs express the OCT4 and REX1 transcription factors, two specific markers of undifferentiated embryonic stem (ES) cells.
- Oct-4-mediated transactivation is stimulated by EWS
- The hOct4 promoter upstream region contains multiple regulatory elements, one of which, the GC box, may be an important cis-regulatory element that regulates the transcription of the hOct4 promoter by the binding of Sp family transcription factors.
- The Oct4 protein co-occupy a substantial portion of its target genes, and collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
- results suggest that pseudogenes Oct4-pg5 and Oct4-pg1 may be involved in the regulation of Oct4 gene activity thus might be pertinent to carcinogenesis
- Expression of POU5F1 isoform A and POU5F1_isoform B was examined in hESCs and all stages of human preimplantation development to look for differences in expression, biological activity, and relation to totipotency.
- DNA binding, transactivation, and abilities to confer self-renewal of the human OCT-4 isoforms differ
- RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells.
- Isolation from cord blood a population of human cells that are similar to murine bone marrow-derived cells containing OCT4.
- OCT-4, an embryonic stem cell marker, is highly expressed in bladder cancer
- Data show that Oct-4, Rex-1, and Gata-4 expression in human mesenchymal stem cells increases cell differentiation efficiency but not hTERT expression.
- These data indicate that SF-1 plays a crucial role in the regulation of hOct4 transcription through direct binding to the 1st SF-1 in Conserved Regions 1 of the hOct4 proximal promoter.
- POU5F1 and POU2F subfamily members play a pivotal role for the FZD5 expression in undifferentiated human ES cells, fetal liver/spleen, adult colon, pancreatic islet, and diffuse-type gastric cancer
- genetic association study shows that only a haplotype containing Cw*0602 is strongly associated with psoriasis vulgaris in Chinese; POU5F1 gene is unlikely to be the disease gene at PSORS1 locus
- reprogramming of DNA methylation and histone modifications on regulatory regions of the developmentally regulated OCT4 and NANOG genes
- Human peripheral blood mononuclear cells, genetically stable and mainly terminally differentiated cells with well defined functions and a limited lifespan, express Oct-4.
- WNT8B expression in hepatocyte progenitors derived from human ES cells is due to POU5F1 (OCT3/OCT4) and GATA3, and WNT8B expression in diffuse-type gastric cancer is due to POU5F1 and GATA6
- Stem cell marker expressed in dental pulp stem cells from 5-7 year olds.
- Our results suggest that an immunohistochemical panel consisting of TTF-1, CEA, CA-125, and OCT-4 is helpful in distinguishing most pulmonary and ovarian carcinomas with clear cell features.
- RNAi-mediated silencing of OCT4 induced differentiation with mesodermal characteristics in EC cells
- sumoylation of Oct4 results in increased stability, DNA binding, and transactivation and provides an important mechanism to regulate Oct4 activity
- Differential methylation of the OCT3/4 upstream region is associated with primary testicular germ cell tumors
- In pluripotent human embryonic stem cells (unlike differentiated hES) Oct4 genes were located on greatly extended chromatin loops, outside their respective chromosome sites, suggesting role in pluripotency of hES.
- possible role of the OCT-4 protein in seminomas
- OCT4 targets similiar genes in mesenchmal and embryonic stem cells and promotes the expression of specific genes and cell cycle progession in each type of cell.
- SARS-infected lung cells express the stem/progenitor cell markers CD34 and Oct-4.
- Both upregulation and downregulation of Oct4 in human embryonic stem cells results in differentiation, but with patterns distinct from parallel experiments in mice.
- OCT4 expression in the cervical carcinoma cell line HeLa and the breast cancer cell line MCF7 was determined in comparison with the human teratoma cell line nTera.
- OCT4 mRNA and protein were detected in extracts from both 1st and 2nd trimester ovaries and testes.
- Using ectopic expression of Oct4, Sox2, Klf4 and Myc, we have derived iPS cells from fetal, neonatal and adult human primary cells
- The human primordial germ cells is the first primary cell type described to express POU5F1 and NANOG but not SOX2.
- POU5F1 is highly polymorphic, however a smaller subset of polymorphisms can tag the observed genetic variation with little loss of information
- OCT-4 expression was not detected in patients with basal-like phenotype of breast cancer.
- We conclude that the proliferation of immature germ cells in GB may be due to an interaction between OCT3/4 and accumulated beta-catenin in the nuclei of the immature germ cells.
- A comparatively homogeneous population of multipotent adult progenitor cells are able to transcripbe Oct4 protein.[REVIEW]
- Thsi study identifies DPPA4 and other genes as putative Sox2:Oct-3/4 target genes using a combination of in silico analysis and transcription-based assays.
- Sox2, Oct4 and Nanog are linked together in a pluripotent regulatory network
- Immunohistochemical localization of Oct4 in stem cell compartments of human sacrococcygeal teratomas
- Oct4 is down-regulated by hypermethylation in normal placenta and gestational trophoblastic diseases.
- Differential expression of the pluripotency structural gene OCT4 in human preimplantation development.
- Oct-4 and Nanog may have roles in oral cancer stem-like cells and high-grade oral squamous cell carcinoma
- Oct-4 expression plays a crucial role in maintaining the self-renewing, cancer stem-like, and chemoradioresistant properties of LC-CD133(+).
- MSC stemness does not correlate to expression of POU5F1 transcripts or its pseudogenes.
- results implicate that Oct-3/4 may be useful as a novel tumor biological and prognostic marker and probably as a potential therapeutic target for bladder cancer
- The transcriptional activation of miR-302 and the translational repression of its targets, such as cyclin D1, may provide a link between Oct4/Sox2 and cell cycle regulation in pluripotent cells.
- Novel variants of Oct-3/4 gene expressed in mouse somatic cells
- The hOct4 promoter is differently regulated in mouse cells.
- Identification of the OCT4-pg1 retrogene and NANOG gene expression in the human embryonic eye
- Esrrb coordinates with Nanog and Oct4 to activate the internal machinery of ES cells
- analysis of expression of OCT4 in tumor cell lines from 13 entities & bone marrow-derived mesenchymal stem cells (MSC); with exception of typical embryonal carcinoma cells, we did not observe reliable OCT4 expression in somatic tumor cell lines & MSC
- The increased number of cells that expressed Oct4A in prostate cancer compared to benign prostate, and in cancers of increasing grade, suggests that Oct4A/Chromogranin A co-expressing cells represent neuroendocrine cells in prostate cancer.
- we show that association of Oct4 and Oct1 with a distinct group of in vivo targets is inducible by stress, and that Oct1 is essential for a normal post-stress transcriptional response