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Validated All-in-One™ qPCR Primer for ASNS(NM_001673.5) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Summary
The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. There are three alternatively spliced transcript variants encoding the same protein described for this gene.
Gene References into function
- Role of Sp1 and Sp3 in the nutrient-regulated expression of the human asparagine synthetase gene
- ATF4 is a mediator of the nutrient-sensing response pathway that activates asparagine synthetase gene
- Genomic sequences necessary for transcriptional activation by amino acid deprivation of mammalian cells. activation of asparagine synthetase gene by endoplasmic reticulum stress.
- documentation of the comparison between CHOP and AS transcriptional control elements used by the amino acid pathway
- ATF3-FL and C/EBPbeta act as transcriptional suppressors for the ASNS gene to counterbalance the transcription rate activated by ATF4 following amino acid deprivation
- The Asparagine Synthetase), which is a housekeeping gene, is repressed and the promoting region of the gene is highly methylated.
- coorelation between methylation of ASNS and acute lymphoblastic leukemia
- ASNS RNAi potentiates L-asparaginase chemotherapeutic activity in ovarian cancer cell lines from the NCI-60, and ASNS mRNA expression predicts L-asparaginase activity in the ovarian subpanel of the NCI-60.
- results suggest that the presence of MELAS and NARP mtDNA mutations elicits upregulation of CHOP and ASNS genes through the elevation of ATF4 expression and its binding to the amino acid regulatory element and nutrient-sensing response element-1
- RNA interference decreases capacity of mesenchymal stem cells to protect acute lymphoblastic leukemia cells from asparaginase, whereas enforced ASNS expression conferred enhanced protection.
- ASNS (asparagine synthetase) transcription activity was up-regulated in HepG2 cells treated with the UPR activators thapsigargin and tunicamycin
- ASNS protein expression predicts L-asparaginase chemotherapeutic activity in a large, diverse set of ovarian cancer cell lines.
- These findings provide rationale for evaluation of ASNS protein expression as a predictive biomarker of clinical L-ASP activity in ovarian cancer.
- CHOP is a member of the transcription factor network that controls the stress-induced regulation of specific C/EBP-ATF4-containing genes, such as ASNS