|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for ITGA5(NM_002205.4) Search again
Product ID:
HQP117778
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD49e, FNRA, VLA-5, VLA5A
Gene Description:
integrin subunit alpha 5
Target Gene Accession:
NM_002205.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].
Gene References into function
- alternative pathway of pro-alpha(v) processing involving MT1-MMP: regulation of integrin functionality may be an important role of MT1-MMP in migrating tumor cells.
- The PDZ domain of TIP-2/GIPC interacts with the C-terminus of the integrin alpha5 and alpha6 subunits.
- role in mediating adhesion and migration of activated cycling CD34+ hematopoietic progenitor cells onto fibronectin
- Integrin activation involves a conformational change in the alpha 1 helix of the beta subunit A-domain
- results suggest that the inadequate trophoblastic invasion, induced by antiphospholipid antibodies, can be the result of decreased alpha1 integrin and VE-cadherin and increased alpha5 integrin and E-cadherin expression in the trophoblast
- Central role of alpha5beta1 integrin in vascular development in mouse embryos and embryoid bodies.
- TGF-beta1-stimulated osteoblasts require intracellular calcium signaling for enhanced alpha5 integrin expression
- The alpha5beta1 integrin provides matrix survival signals for normal and osteoarthritic human articular chondrocytes in vitro.
- results suggest that engagement of the alpha 5 beta 1 integrin promotes an NF-kappa B-dependent program of gene expression that coordinately regulates angiogenesis and inflammation
- Expression of focal adhesion kinase and alpha5 and beta1 integrins in carcinomas and its clinical significance.
- B. pertussis FHA up-regulates ICAM-1 expression on respiratory epithelial cells through interaction of its RGD site with host cell VLA-5 integrin, and that PT impairs this response
- Elevated expression of IL-8 (and not PTHrP) by MDA-MET cells is a phenotypic change that may be related to their enhanced ability to metastasize to the skeleton.
- integrin alpha5beta1 extracellular domain controls rhoA activity
- Alterations in alpha5beta1 levels induced by TGF-beta are mediated at least in part through the induction of CTGF in glomerular mesangium
- thymidine phosphorylase and 2-deoxyribose-induced focal adhesion kinase phosphorylation was blocked by the antibodies to integrins alpha 5 beta 1 and alpha v beta 3, directly linking their migration and signaling components
- endostatin competes with fibronectin/RGD cyclic peptide to bind alpha 5 beta 1 integrin
- identification of binding site in gamma C-domain of fibrinogen
- alpha 5 beta 1 and alpha v beta 3 are both important but cell-specific fibrillin-1 receptors
- VEGFR-1 secreted by endothelial cells interacts with the alpha 5 beta 1 integrin
- visualized the head region of integrin alpha 5 beta 1 in an inactive (Ca2+-occupied) state, and in complex with a fragment of fibronectin containing the RGD and synergy recognition sequences
- The structural conformation of this protein, complexed wigh integrin beta1, complexed with fibronectin, is examined.
- Disulfide bonding patterns of the integrin alpha 5 chain are demonstrated; new insights into a second bond between the heavy and light chains of alpha 5 are described.
- Hox D3 coordinately regulates the expression of integrin alpha5beta1 and integrin alphavbeta3 during angiogenesis in vivo.
- Integrin alpha5beta1 and ADAM-17 interact in vitro and co-localize in migrating HeLa cells
- beta1,4-N-acetylglucosaminyltransferase III inhibits the function of alpha5beta1 integrin
- Role of alpha4, alpha5, and alphav integrin receptors--which are central to mediating interactions with these domains of FN--in regulating ssquamous cell carcinoma migration.
- findings identify Glu-Pg as an adhesive ligand for integrins alphaMbeta2 and alpha5beta1 and suggest that alpha5beta1 may participate in the binding of soluble Glu-Pg and assist in its activation.
- The marked decrease in integrin alpha5beta1 expressions was unique to aneurysmal aortic tissues and correlated to a decrease in density of smooth muscle cells
- results indicate a new interaction partner for the alpha(v)beta(3) integrin, the TGFbetaIIR, in which TGFbeta1-induced responses are potentiated in the presence alpha(v)beta(3) ligands
- Data show that squamous cell carcinoma cells escape suspension-induced, p53-mediated anoikis by forming multicellular aggregates that use fibronectin survival signals mediated by integrin alpha(v) and focal adhesion kinase.
- integrin alpha5beta1 participates in the activation of both VEGFR-3 and its downstream PI3 kinase/Akt signaling pathway, which is essential for fibronectin-mediated lymphatic endothelial cell survival and proliferation.
- The integrin, alpha 5 was expressed by prostate cancer cells in vitro and by prostate tumors in vivo, and their expression was elevated at sites of bone metastasis compared to original prostate tumor.
- Insulin-like growth factor I controls adhesion strength mediated by alpha5beta1 integrins in tumor cells
- Direct binding and regulation of alpha5beta1 by uPAR implies a modified "bent" integrin conformation can function in an alternative activation state with this and possibly other cis-acting membrane ligands.
- Alpha5beta1 integrin is required for up-regulation of IL-1 beta-dependent airway smooth muscle secretory responses by fibronectin.
- the alpha5 integrin subunit has a role in the induction of apoptosis triggered by FGFR2 activation in osteoblasts, and a Cbl-dependent mechanism is involved in the coordinated regulation of cell apoptosis induced by alpha5 integrin degradation
- Alpha5beta1-mediated adhesion of Ntera2 neuronal cells to fibronectin decreased apoptosis in response to serum withdrawal.
- integrin alpha5/beta1 exerts its tumor suppressor-like activity in colon cancer cells by inhibiting HER-2 signaling
- findings suggest that only some P. aeruginosa strains can target Fn and their natural receptors alpha5beta1 integrins for adherence to A549 cells
- alpha3, alpha5, and alpha6 beta1 integrins are expressed in ductal cells at 8 weeks, before glucagon- and insulin-immunoreactive cells bud off in fetal pancrea.
- ITGA5 mediates engraftment of GCSF-mobilized stem cells.
- integrin alphavbeta6, alphavbeta3, alphavbeta5, alpha5beta1 and alpha9beta1 binding to osteopontin is controlled by specific structural motifs that are recognized by extracellular proteases
- The integrin receptors of tenascin (TN) and fibronectin (FN) may be involved in cell processes such as growth, adhesion, migration and apoptosis.
- CCN2/CTGF enhances chondrocyte adhesion to FN through direct interaction of its C-terminal CT domain with FN, and alpha5beta1 is involved in this adhesion.
- urokinase plasminogen receptor domain III controls a functional association with alpha5beta1 integrin and tumor growth
- one mechanism by which IGFBP2 activates IGFBP2-induced cell mobility is through its interaction with integrin alpha5 and this interaction is specifically mediated through the RGD domain on IGFBP2
- binding of CXCL12 to its receptor leads to enhanced expression of alpha5 and beta3 integrins
- Alpha5beta1 (VLA-5) integrin is a major galectin-1-glycosylated counterreceptor involved in immune developmental synapse formation.
- This is the first report of direct binding of a Mycobacterium tuberculosis molecule to a receptor on human T cells resulting in a change in CD4+ T cell function
- the internalization of AAV2 in mammalian cells might follow a "click-to-fit" mechanism that involves the cooperative binding of heparan sulfate and alpha5beta1 integrin by the AAV2 capsids
- activities for cell spreading and migration for the alpha5 subunit carrying only three potential N-glycosylation sites (3-5 sites) on the beta-propeller were comparable with those of the wild type
- integrin alpha5beta1 plays a pivotal role in the hypotonic stress-induced but not in the lysophosphatidic acid-induced responses
- These findings suggest that interaction with fibronectin through integrin alpha5 plays an important role in human extravillous trophoblast invasion.
- surface micron-scale topography modulates alpha(5)beta(1) integrin binding and FAK activation
- CEA-mediated signaling involves clustering of CEA and co-clustering and activation of the alpha5beta1 and associated specific signaling elements on the internal surfaces of membrane microdomains
- integrins alpha5beta1 and alphaV are inhibited by lebectin and lebecetin snake venom lectins
- Interaction of alpha-toxin with alpha5beta1-integrin and overproduction of TNF-alpha may contribute to destruction of epithelial cells during S. aureus infection.
- Modulation of cell adhesion is a normal physiological action of PTHrP, mediated by increasing integrin gene transcription.
- This study identified the beta1 integrins on primary smooth muscle cells that fibulin-5 interacts with, and showed that failure of fibulin-5 to activate these receptors limits cell spreading, migration and proliferation.
- OPN plays a crucial role for tumor growth and angiogenesis of human lung cancer cells in vivo by interacting with alphavbeta3 integrin.
- Integrin alpha5beta1 is critical for the chondrocyte hypertrophic differentiation induced by GTP-bound transglutaminase 2, and this induction is ligand dependent.
- findings suggest possible participation of alpha5beta1 integrin in the mechanism of local invasion of ameloblastomas
- KGF increased integrin alpha(5) expression by phosphorylating C/EBP-beta
- There is low expression of the proteins and mRNA of ME491/CD63 and integrin alpha5 in ovarian cancer. The lower the pathological differentiation is, the more significant the loss of expression is and the more likely metastasis is.
- Protein expression of ITGA5 decreased at labour and expression of these molecules was also observed in primary cultured human uterine SMCs.
- Notch1 ligand, Delta-like ligand-4, stimulates R-Ras-dependent alpha 5 beta 1 integrin-mediated adhesion, demonstrating the physiological relevance of this pathway.
- Integrin alpha5 subunit plays a significant role in TGF-beta1-induced fibronectin deposition on human airway smooth muscle.
- analysis of important residues within the central turn motifs present in the cytoplasmic tails of integrin alphaIIb and alphaV subunits
- data identify PAI1 as a novel regulator of fibronectin matrix assembly, and indicate that this regulation occurs through a previously undescribed crosstalk between the alphavbeta5 and alpha5beta1 integrins
- This is the first time to report that alpha1,3FucT-VII can regulate the mRNA expression of integrin.
- Both integrins, v3 and v5, are involved in the glioma cell radioresistance through the integrin-linked kinase (ILK) and the small GTPase RhoB, at least by regulating the radiation-induced mitotic cell death.
- SPARC-mediated mobilization of adipose stromal (stem) cells through its effect on alpha5beta1 integrin complex provides a functional basis for the regulation of white adipose tissue body composition by SPARC.
- Oxidized LDL impairs angiogenic properties of endothelial progenitor cells at sub-apoptotic levels by downregulation of E-selectin and integrin alphavbeta5, both substantial mediators of EPC-endothelial cell
- The ability of alpha5 beta1 integrin to interact with soluble fibronectin may thus drive the cell-matrix adhesion and cytoskeletal organization required for a contractile, fibroblast-like morphology.
- Generated high throughput platform to examine interactions of type I collagen receptor alpha(2)beta(1) and fibronectin receptor alpha(5)beta(1) with peptide ligands to evaluate the effects of integrin cross-talk on adhesive responses.
- EGF cooperates with alpha(5)beta(1) integrin signaling to induce EMT in cervical cancer cells via up-regulated Snail
- Extracellular Cyr61 interacts with alpha(5), alpha(6), and beta(1) integrins
- Data suggest that alpha5beta1 intergrin controls invasion ability of MCF-7/ADR breast carcinoma cells by regulating expression of MMP-2, which involves PI-3K and Erk1/2 protein kinase signaling.
- These results suggest that alpha5 gene expression is likely dictated by subtle alterations in the nuclear ratio of TFs that either repress (NFI) or activate (Sp1 and AP-1) alpha5 transcription in corneal epithelial cells.
- GnT-V is expressed in human extravillous trophoblast and is involved in regulating trophoblast invasion through modifications of the oligosaccharide chains of alpha5beta1 integrin.
- findings show that alpha(5)beta(1) integrin switches between relaxed and tensioned states in response to myosin II-generated cytoskeletal force