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Validated All-in-One™ qPCR Primer for CEBPA(NM_004364.4) Search again
Product ID:
HQP117752
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
C/EBP-alpha, CEBP
Gene Description:
CCAAT enhancer binding protein alpha
Target Gene Accession:
NM_004364.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Summary
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq].
Gene References into function
- Down-regulation and antiproliferative role of C/EBPalpha in lung cancer.
- may function as a tumor suppressor that is mutated during tumorigenesis. Abnormalties in C/EBPalpha function contribute to the development of malignancies in a variety of tisues.
- Surfactant protein D gene regulation. Interactions among the conserved CCAAT/enhancer-binding protein elements.
- Ras signaling enhances the activity of C/EBP alpha to induce granulocytic differentiation by phosphorylation of serine 248.
- Conserved amino acids regulate phosphoenolpyruvate carboxykinase (PEPCK) gene expression.
- expression level of the C/EBPalpha gene in hepatocytes was downregulated in response to proliferation signals
- Conditional expression of C/EBP alpha induced the C/EBP family members C/EBP beta and C/EBP epsilon and subsequent granulocyte differentiation.
- role in mediating GADD153 expression is involved in deoxycholic acid-induced apoptosis
- Novel protein kinase C isoforms regulate human keratinocyte differentiation by activating a p38 delta mitogen-activated protein kinase cascade that targets this protein
- C/EBPalpha and PU.1 interact physically and colocalize in myeloid cells, and C/EBPalpha blocks the function of PU.1.
- C/EBPalpha is essential for p21-mediated inhibition of G1 to S-phase progression by RAP in KSHV-infected host cells
- Calreticulin interacts with C/EBPalpha and C/EBPbeta mRNAs and represses translation of C/EBP proteins.
- favorable prognostic significance of mutations in patients with de novo acute myeloid leukemia
- CEBPA and CEBPe repress the leukemic phenotype of acute myeloid leukemia, suppress cell growth, and induce partial differentiation.
- 5'-region from -252 to -175, containing a consensus site for CCAAT/enhancer binding proteins alpha,beta (C/EBPalpha,beta), was essential for SAA1 induction in HASMCs.
- the level of basal as well as cAMP-stimulated IL-10 transcription depends on the expression of C/EBP alpha and beta and their binding to three motifs in the promoter/enhancer region
- CCAAT/enhancer-binding protein alpha has a synergy control motif that inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3
- C/EBP alpha binds to the lactoferrin promoter in nonexpressing cells.
- affects on JunB expression and monocyte differentiation
- The intimate association of Pit-1 and C/EBPalpha at certain sites within the living cell nucleus could foster their combinatorial activities in the regulation of pituitary-specific gene expression.
- The emergence of a mutation in this protein is involved in the clonal evolution of myelodysplastic syndromes towards secondary acute myeloid leukemia.
- C/EBP-alpha has been identified as a major activator of the human myeloid IgA Fc receptor promoter.
- Mutation of CEBPA is a recurrent finding in acute myeloid leukemia FAB type M1 and M2 and appears specific to the intermediate cytogenetic risk group patients.
- C/EBP alpha and HNF-3 gamma cooperatively regulate CYP3A4 expression in hepatic cells by a mechanism that probably involves chromatin remodeling.
- C/EBP alpha may play role in the regulation of the resistin gene expression
- Expression levels of both C/EBPalpha isoforms in breast tumors were correlated with clinicopathological tumor parameters, expression of estrogen and progesterone receptors (ER, PR), Ki67 immunostaining, and expression of 7 cell-cycle regulatory proteins.
- C/EBP and RUNX/AML factors compete for binding to their respective cognate elements and bind to the CD11a promoter MS7 sequence in a cell lineage- and differentiation-dependent manner.
- several regions of the C/EBP alpha protein are involved in inhibition of proliferative pathways in granulopoiesis
- CCAAT/enhancer-binding protein-alpha is induced during the early stages of Kaposi's sarcoma-associated herpesvirus lytic cycle reactivation
- We conclude that C/EBPalpha and C/EBPbeta contribute to the deregulated expression of Bcl-2 in t(14;18) lymphoma cells
- C/EBPalpha has a role in neutrophil differentiation
- a novel mechanism for IL-6-mediated repression of gene transcription that involves a reduction in C/EBPalpha-mediated activation
- Expression of mutated CEBPA in human CD34+ cord blood cells dramatically inhibited differentiation of both myeloid and erythroid lineages.
- phosphorylation induces conformational changes in C/EBP alpha, increasing the distance between the amino termini of C/EBPalpha dimers. This favors monocyte differentiation by blocking granulopoiesis.
- Mutant CEBPA predicts favorable prognosis and may improve risk stratification in acute myeloid leukemia patients with normal cytogenetics.
- selective inhibition of G-CSF receptor expression by C/EBPalphap30-ER is due in part to its variable affinity for C/EBP sites
- C-EBP has an essential role in regulating PDGFRalpha expression
- interacts with Epstein-Barar virus ZTA protein through oligomerization and transactivates ZTA promoter by binding ZII and ZIIIB motifs during lytic cycle induction
- CCAAT/enhancer-binding protein alpha (C/EBPalpha) activates transcription of the human microsomal epoxide hydrolase gene (EPHX1) through the interaction with DNA-bound NF-Y
- Alterations in C/CAAT enhancer binding protein alpha and neuronal apoptosis inhibitory protein expression occurred in human adipose stromal-vascular cells after weight loss
- C/EBPbeta enhancer, prolactin, significantly induced extracellular superoxide dismutase mRNA and protein.
- CEBPA is a DNA damage-inducible p53-regulated mediator of the G1 checkpoint in keratinocytes.
- CEBPA mutation is apparently the primary event in the development of AML in this family.
- The CCAAT-Enhancer-Binding Protein-alpha is involved in granulocytic differentiation of common myeloid progenitors.
- one of the major roles of Stat3 in the G-CSF signaling pathway is to augment the function of C/EBPalpha, which is essential for myeloid differentiation
- The CCAAT/enhancer-binding protein-alpha (CEBPA) is a transcription factor strongly implicated in myelopoiesis through control of proliferation and differentiation of myeloid progenitors.
- Phosphorylation-dependent switch of biological functions of C/EBPalpha promotes liver proliferation.
- Findings implicate the CEBPA mutation as a potential marker for monitoring minimal residue disease.
- Activation by C/EBP alpha and beta did not depend on their binding to the C/EBP site, since they still activated IGFBP-5 promoter.
- modulation of CEBPA by calreticulin represents a novel mechanism involved in the differentiation block in CBFB-SMMHC AML.
- Results suggest that reduced expression of C/EBPalpha may play a role in the development and/or progression of breast cancer.
- C/EBPalpha is a key transcription factor for full activation of human adiponectin gene transcription in mature adipocytes through interaction with response elements in the intronic enhancer.
- there is a physical interaction between the GABPalpha subunit and C/EBPalpha
- The inhibition of HL-60/FAK differentiation resulted from both the induction of pRb hyperphosphorylation and the inhibition of association of pRb and c/EBPalpha.
- Data show that human granulocytic differentiation is controlled by a regulatory circuitry involving miR-223 and two transcriptional factors, NFI-A and C/EBPalpha.
- down-modulation of C/EBPalpha is a prerequisite for STAT5-induced effects on self-renewal and myelopoiesis in human cord blood-derived stem/progenitor cells
- DNA hypermethylation of the upstream C/EBPalpha promoter region, not the core promoter region as previously reported, is critical in the regulation of C/EBPalpha expression in human lung cancer.
- CA150 is a co-repressor of C/EBP proteins and provides a possible mechanism for how C/EBPalpha can repress transcription of specific genes
- CCAAT/enhancer binding proteins alpha and epsilon cooperate with all-trans retinoic acid and show antitumor activity
- C/EBP alpha activates the latent myeloid differentiation program of megakaryocyte/erythroid progenitors and common lymphoid progenitors in transgenic mice; its global activation affects multilineage homeostasis in vivo.
- CEBPA is activated by juxtaposition to the immunoglobulin gene enhancer upon its rearrangement with the immunoglobulin heavy-chain locus in precursor B-cell acute lymphoblastic leukemia harboring t(14;19)(q32;q13).
- HNF4alpha, CREM, HNF1alpha, and C/EBPalpha have roles in transcriptional regulation of the glucose-6-phosphatase gene by cAMP/vasoactive intestinal peptide in the intestine
- C/EBPalpha protein half-life leading to its enhanced transactivation and DNA-binding capacity is prolonged by JNK1
- Max as a novel co-activator of C/EBPalpha functions, thereby suggesting a possible link between C/EBPalpha and Myc-Max-Mad network.
- These results indicate that C/EBPalpha regulates AFP gene expression through direct binding to multiple sites in the human AFP gene in cultured human cells.
- The HP196-197 insertion is not a mutation but a polymorphism and does not appear related to acute myeloid leukemia.
- CCAAT/enhancer binding proteins alpha and beta have roles in growth and differentiation of hepatoblastomas
- ATF4 may regulate myeloid gene expression differentially by potentiating C/EBPepsilon but inhibiting C/EBPalpha-mediated transcriptional activation.
- Activation of the CCAAT/enhancer binding protein alpha promoter was mediated by PI3 kinase.
- Low C/EBPalpha is associated with myelopoiesis
- diacylglycerol acyltransferase 2 expression is regulated by CAAT/enhancer-binding protein beta (C/EBPbeta) and C/EBPalpha during adipogenesis
- homozygous CEBPA mutations in acute myeloid leukemia may be due to segmental uniparental disomy
- C/EBPalpha elicits a role as an effector downstream to HIF-1alpha in myeloid leukemic cells differentiation.
- Acute myeloid/T-lymphoid leukemia with silenced CEBPA and mutations in NOTCH1.
- C/EBPalpha binds and activates the PU.1 distal enhancer to induce monocyte lineage.
- Ubc9 is an important C/EBPalphap30 target through which C/EBPalphap30 enhances the sumoylation of C/EBPalphap42 to inhibit granulocytic differentiation.
- In a cohort of 305 patients with de novo acute myeloid leukemia (excluding APL), the 6 bp insertion in the TAD2 domain did not have prognostic significance. It was also seen in some healthy controls.
- role of C/EBPalpha in the induction of the IGnTC gene as well as in I antigen expression
- Inhibition of C/EBPalpha function may be causatively related to the leukemogenic potential of RUNX1/EVI1 chimeric transcription factor.
- The effect of cell density and inflammatory conditions on the expression, compartmentalization, activation, and the anti-proliferative function of the GR in primary human lung fibroblast cultures, was studied.
- CCAAT/enhancer-binding protein alpha antagonizes transcriptional activity of hypoxia-inducible factor 1 alpha with direct protein-protein interaction
- four types of polymorphisms and 25 mutations were detected in CEBPA in samples from 390 patients with myelodysplastic syndrome (MDS) and hematologic malignancies
- C/EBPalpha regulates FAT/CD36 gene expression at the transcriptional level.
- Based on our observations in the present study, we conclude that S248A mutation of C/EBPalpha leads to a reduction of granulocytic differentiation markers and a block in differentiation at the morphological level.
- Low CEBPA levels were associated with leukopenia
- These data show that apoptotic and growth inhibitory activities of C/EBPalpha are differentially regulated in different cells and that cooperation of cyclin D3 and C/EBPalpha is required for the inhibition of proliferation.
- review of roles of cebpa and tip60 in genetics of leukemiogenesis
- Epigenetic alterations of C/EBP alpha are a frequent event in acute myeloid leukemia.
- In malignant prostate C/EBPalpha may be available to regulate androgen receptor signaling through transient changes in its sub-cellular localization
- Transcription of the invasion suppressor, CRMP-1, is reciprocally regulated at the promoter region by C/EBPalpha and Sp1.
- Data show that the CEBPA mutation K313dup is a recurrent CEBPA mutation in de novo acute myeloid leukemia.
- CEBPA polymorphisms occurred more frequently than CEBPA mutations and could be identified across all prognostic risk groups
- Aberrant methylation of the C/EBPalpha promoter region occurred in 10/80 diagnostic AML samples, and there was an inverse correlation between aberrant methylation of C/EBPalpha and the negative cell cycle regulator p15.
- Adipose tissue C/EBPalpha regulates several genes in glucose and lipid metabolism
- Data strongly indicate that germline CEBPA mutations predispose to acute myeloid leukemia and that additional somatic CEBPA mutations contribute to the development of the disease.
- ectopic expression of C/EBPepsilon, as well as C/EBPalpha, can induce the monocytic differentiation of myelomonocytic leukemic cells with MLL-fusion gene through the downregulation of Myc
- NRAMP1 proximal region binds CCAAT enhancer binding proteins alpha or beta and is crucial for transcription
- Report the prognostic significance of, and gene and microRNA expression signatures associated with, CEBPA mutations in cytogenetically normal acute myeloid leukemia with high-risk molecular features.
- Hybrids of the bHLH and bZIP protein motifs display different DNA-binding activities in vivo vs. in vitro.
- aberrant silencing, as well as, inappropriate cytoplasmic localization of C/EBPalpha causes dysregulation of its function, suggesting that C/EBPalpha is a novel candidate tumor suppressor gene in pancreatic cancer cells
- AP2alpha suppresses C/EBPalpha promoter activity and protein expression in head and neck squamous cell carcinoma.