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Validated All-in-One™ qPCR Primer for CD14(NM_000591.3) Search again
Product ID:
HQP116308
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
-
Gene Description:
CD14 molecule
Target Gene Accession:
NM_000591.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq].
Gene References into function
- CD14-dependent activation of NF-kappaB by filarial parasitic sheath proteins
- Data suggest that soluble lipopolysaccharide receptor CD14 (sCD14) elevation is associated with the development of multiple organ dysfunction syndrome (MODS) after major burns.
- results suggest that the enhanced CD14 expression in psoriasis is not attributable to functional variants of CD14 (-159C/T)
- IFN-gamma primed CD14(high) human gingival fibroblasts to enhance production of IL-8 in response to LPS through augmentation of the CD14-TLR system
- The important host receptor CD14 is partially implicated in TNF, IL-1, IL-6 and MCP-1 production, while CD14-independent pathways seem to be responsible for IL-8 production after S. suis stimulation.
- Though Cd14 is known to faciitate binding of several bacterial ligands to TLR, no role could be found for CD14 in C. pneumoniae binding.
- No association between the CD14 C(-260)T and CD18 codon 441 gene polymorphisms and the incidence of stroke was found in a large, prospective, matched case-control sample from the Physicians' Health Study.
- CD14 polymorphism is associated with smoking dependence
- Human proteinase 3 can inhibits LPS-mediated TNF-alpha production through CD14 degradation
- plasma factor LBP and cell surface receptor CD14 were necessary for LPS activation of p38, which was tightly associated with LPS priming of the PMN respiratory burst
- TT genotype of CD14 associated with risk of atherosclerotic or microangiopathic stroke
- CD14 has a role in mediating signaling responses to Aspergillus fumigatus
- Data show that the TLR4-mediated LPS response in bladder epithelial cells also uses the co-receptor CD14 for efficient LPS signalling.
- Porphyromonas gingivalis fimbriae activate human peripheral blood monocytes utilizing TLR2, CD14 and CD11a/CD18 as cellular receptors.
- Lipopolysaccharide rapidly traffics to and from the Golgi apparatus with the toll-like receptor 4-MD-2-CD14 complex
- some heat-stable component of P. gingivalis, including LPS, may be responsible for the induction of IL-8 and MCP-1 in HUVECs by a CD14-dependent mechanism
- lipopolysaccharide may induce proliferation of periodontal epithelial cells by upregulating keratinocyte growth factor 1 expression via the CD14 and Toll-like receptor signaling pathway
- innate immune recognition of LTA via LBP, CD14, and TLR-2 represents an important mechanism in the pathogenesis of systemic complications in the course of infectious diseases brought about by Gram-positive pathogens. while TLR-4 and MD-2 are not involved
- The CD14 -159C/T polymorphism is associated with increased common carotid artery intima-media thickness in smokers
- saliva CD14 is important for the maintenance of oral health and possibly intestinal homeostasis.
- Human cytomegalovirus activates inflammatory cytokine responses via CD14 and Toll-like receptor 2
- first study to suggest an effect of a genetic polymorphism of the CD14 gene on both insulin sensitivity (healthy subjects and type 2 diabetic patients) and endothelial dysfunction (sICAM-1 levels) in type 2 diabetes mellitus
- CD14 is not critical for phosphatidylserine-induced apoptosis in human macrophages
- a new functional role of CD55 as a member of a multimeric LPS receptor complex.
- data support the hypothesis that lipopolysaccharide binding protein can inhibit cell responses to lipopolysaccharide(LPS) by inhibiting LPS transfer from membrane CD14 to the Toll-like receptor 4-MD-2 signaling receptor
- data suggest that peripheral blood cells are of negligible importance in LPS-induced production of inflammatory mediators in vivo and that LPS may activate genes via a CD14-independent pathway that is slower and less efficient
- A significant association between the CD14 TT genotype and C. pneumoniae infection was found.
- there may be a resistive exercise training-induced reduction in TLR4/CD14 expression in older women
- results provided experimental evidence for existence of close association between CD14 and HIV coreceptor CXCR4 on human monocytic cells
- The TT genotype of -159 C-->T CD14 is associated with nonatopic asthma and food allergy, particularly in white subjects. Thus CD14 is a candidate gene specifically for nonatopic asthma and not for asthma in general
- The polymorphic maeker C-159T of CD14 was associated with serum total IgE concentration in atopic Chinese children.
- increased serum levels in chronic Hepatitis B and C patients and inversely correlated with HBsAg in chronic Hepatitis B
- Results show that the surface receptors TLR2/4 and CD14 are essential for in vitro cellular activation induced by Neisseria meningitidis lipopolysaccharide-containing outer membrane vesicles and purified lipopolysaccharides.
- A marker on monocytes that can differentiate into several distinct mesenchymal cell lineages.
- saliva CD14 promoted the invasion of oral epithelial cells by A. actinomycetemcomitans and consequently augmented the production of IL-8, playing an important role in innate immunity in the oral cavity
- soluble CD14 is associated with aortic stiffness independently of age, BP and atherosclerosis in humans.
- the CD14-159 C --> T polymorphism may be an important genetic trait, related to the ability of CD14 to bind and transport lipids, such as cholesterol
- association between the C(-260)T polymorphism in the CD14 promoter and acute myocardial infarction
- CD14 promoter region polymorphism in women with idiopathic recurrent miscarriage
- CD14 and TLR2 but not TLR4 play a major role in lipoteichoic acid -mediated effects on PMN and granulocytes
- downregulated by Ehrlichia chaffeensis infection in monocytes
- CD14 can be considered a type 2 acute phase protein (APP) in vivo. Serum levels of CD14 in patients with arthropathies correlates with those of C-reactive protein, an APP, and IL-6, a cytokine known to regulate the synthesis of APP in the liver.
- Involvement of CD14/TLR4, CR3, and phosphatidylinositol 3-kinase in the degradation of IL-1 receptor-associated kinase in response to LPS.
- The lack of association between CD14 genotypes and IgE as well as atopic outcomes in this large German study population seems to indicate that CD14 genotypes may not directly be involved in the development of allergies during childhood.
- Comparison of the genotypes of 242 patients with Guillain-Barre syndrome (GBS) and 210 healthy subjects shows that polymorphisms in CD14 do not confer susceptibility to GBS and are not associated with Campylobacter jejuni infection.
- concluded that the -159T/C polymorphism in the CD14 monocyte receptor gene was not associated with progression of coronary atherosclerosis in this population nor did it influence the efficacy of pravastatin in the treatment of atherosclerosis.
- this study provides evidence that Porphyromonas gingivalis outer membrane vesicles contribute to the loss of membrane-bound CD14 receptors from the surface of macrophage-like cells and that gingipains degrade this LPS receptor
- in a study of 2558 unrelated Caucasians, it is concluded that the C159T variant has no important effect on plasma cholesterol levels
- Although (C260T) polymorphism in CD14 gene in this study is associated with expression of sCD14, no significant association was found between this polymorphism and early markers of atherosclerosis.
- CD14 and CD16 in monocytes may have a role in development of coronary atherosclerosis and expression of TNF-alpha
- the presence of the membrane-anchored, but not the soluble, form of CD14 is a strong factor in IL-8 induction in bladder epithelial cells in response to bacterial components
- Most interestingly, sCD14 levels were regulated differentially by the same genetic variants in plasma and milk
- Polymorphism of the CD14 gene is associated with atopic asthma
- C -159T promoter polymorphism was associated in female but not in male patients with periodontal disease
- The monocytic CD14 expression and serum levels of C-reactive protein, IL-6 and soluble adhesion molecules were higher in the diabetes group than in the group with normal glucose tolerance.
- The polymorphism of CD14 gene may play an important role in the development of airway obstruction among adult male farmers.
- The CD14/-2984 polymorphism but not the CD14/-159 is associated with IgE level in Taiwanese asthmatic children.
- CD14 is commonly expressed in melamona-infiltrating monocytes/macrophages
- co-existence of a mutation in either the TLR4 or CD14 gene, and in NOD2/CARD15 is associated with an increased susceptibility to developing Crohn disease compared to ulcerative colitis
- These results indicate that the CD14 region spanning amino acids 57-64 is critical for interacting with TLR2 and enhancing TLR2-mediated peptidoglycan signaling.
- There was no significant correlation between CD14-159 promoter polymorphism and the severity of pancreatitis
- CD14 polymorphism associated with increased prevalence of gram-negative bacterial infection in critically ill adults
- There was no association found between the CD14-159T allele and Crohns disease or ulcerative coltis nor any interaction with the CARD15 or TNF-alpha alleles.
- findings reveal that mycobacteria promote their uptake through a process of "inside-out" signaling involving CD14, TLR2, PI3K, and cytohesin-1. This converts low avidity CR3 into an active receptor leading to increased bacterial internalization
- gene polymorphism is associated with chronic periodontitis in Caucasian subjects of a north European origin
- studies suggest that SP-A could contribute to modulate Re-LPS responses by altering the competence of the LBP-CD14 receptor complex
- An altered immune response to lipopolysaccharides with influence on the anti-inflammatory therapy seems to play a role in the genetic predisposition to ulcerative colitis for CD14 gene
- Those with the CD14 -159CC genotype had a significantly increased risk of early infection with P.aeruginosa suggesting that CD14 C-159T plays a role in determining the risk of early infection with P.aeruginosa.
- The frequencies of IL-1beta+3 594T, IL-10-1082G and CD14-159T allele were similar in patients with mild or severe pancreatitis and in controls.
- the single-nucleotide polymorphism at CD14/-159 is associated with the development of biliary atresia and idiopathic neonatal cholestasis
- CT genotype of promotor polymorphism C159T in the CD14 gene is associated with lower prevalence of atopic dermatitis and lower IL-13 production.
- Finds that CD14 polymorphisms influence regulators of the inflammation induced by endotoxin in organic dusts
- The genetic determination of the defense mechanisms in Crohn's disease appears to be not associated with the polymorphism of the CD14 genes.
- The important role of CD14 in innate immunity is reviewed here.
- CD14 and TLR2 remained upregulated throughout the experimental period following exposure to Staphylococcus aureus PepG
- CD14-C159T promoter variant influences total IgE levels and also indicates that the T allele has a more profound effect on total IgE in children with atopic asthma
- C-T (-159) polymorphism in the promoter region of the CD14 gene in patients with alcoholic liver disease and chronic hepatitis C virus infection
- The C allele may be associated with the pathogenesis of alcoholic pancreatitis, and alcoholic patients with acute pancreatitis and cirrhosis of liver are probably two different subpopulations.
- The lipopolysaccharide-binding function characteristic of CD14 enables effective host responses to pathogens.
- In patients with community-acquired infection, low on-admission level of monocyte CD14 is related to fatal outcome
- Maternal allergy status may affect allergic risk in offspring through a decreased expression of fetal CD14.
- The T allele of the C-159T polymorphism of CD14 gene may be a risk factor for myocardial infarction.
- The role of the CD14 -260 C>T polymorphism in the susceptibility to and severity (defined as subfertility and/or tubal pathology) of C. trachomatis infection in women is reported.
- Subjects with CD14 -159CC were more likely to have moderate or severe acute asthma.
- significant increase of the combined carriership of the CD14 -260T and TLR9 -1237C alleles in the chronic relapsing pouchitis group suggests that these markers identify a subgroup of patients with a risk of developing chronic or refractory pouchitis
- In conclusion, our results strongly support an important role for endothelial membrane CD14 in the activation of endothelium for leukocyte recruitment.
- Results identify a new lipopolysaccharide-binding domain of CD14 with a strong lipopolysaccharide-neutralizing activity.
- No association between the CD14/C-159T polymorphism and increased risk for the development of rheumatoid arthritis.
- Results suggest that the presence of intact breast milk soluble CD14 in the upper digestive system could promote innate immunity in this low bacteria density lumen.
- Ciprofloaxin suppressed the expression of CD14 induced by lipopolysaccharides in monocytes.
- The IL-6 -174 G/C, IL-10 -1082 G/A and CD14 -260 C/T SNPs may alter risk for sepsis in ventilated VLBW infants
- Multifactor dimensionality reduction (MDR) test was applied to detect epistasis, and identified single-IL4R(Q576R)- and three-IL4R(Q576R), IL5RA(-80), CD14(-260)- locus association models that predict multiple sclerosis risk with 75-76% accuracy.
- findings indicated that the polymorphism of CD14 but not TLR4 Asp299Gly mutation was associated with Chinese patients with colorectal cancer, and the CD14 gene may contribute to the predisposition to colorectal cancer
- Pathogen recognition receptor CD14 is involved in activation of immature monocyte-derived dendritic cells by the envelope protein surface unit (ENV-SU) of multiple sclerosis-associated retroviral element (MSRV) of human endogenous retrovirus.
- New insights are provided into the importance of stoichiometry among the components of the TLR4/MD-2/CD14 complex; supraoptimal CD14 levels do not suppress lipopolysaccharide-induced signaling.
- This study suggests that CD14 directly binds to triacylated lipopeptides and facilitates recognition of the lipopeptides by the TLR2/TLR1 complex without binding to the receptor complex.
- description of a novel feedback mechanism in apoptotic signaling pathway for SGLT-1-dependent cytoprotection; observation suggests new function for CD14 on enterocytes involving induction of caspase-dependent SGLT-1 activity which leads to cell rescue
- A mutation in CD14 is associated with sarcoidosis.
- CD14/-159 polymorphism may play a role in the development of perennial allergic rhinitis in Korean children.
- CD14 plays a role in IgE-mediated allergic diseases, and its gene polymorphisms can be important for manifestation of these disorders
- These findings show the role of a single-nucleotide polymorphism at CD14/-159 on the development of chronic hepatitis B. Endotoxin susceptibility may play a role in the pathogenesis of chronic hepatitis B.
- serum sCD14 levels are increased in MS and correlate inversely with disease activity in relapsing multiple sclerosis patients
- A marker that may be used used to detect macrophages and in the anatomical plane of the fetomaternal junction.
- no association of CD14 or TLR4 polymorphisms with H. pylori-related gastric malignancy.
- further evidence is provided for the role of CD14 C(-260)--> T promoter polymorphism as a genetic susceptibility marker of atherosclerosis
- pathogenetic role of the CD14 C-260T polymorphism in atherosclerosis as -260TT genotype may favour increased inflammation in atheroma promoting possible worsening atherosclerosis
- PI3 kinase effectors PKB and PKC(zeta) are also activated by PI3 kinase in a CD14 dependent manner in lipopolysaccharides stimulated human peripheral blood mononuclear cells.
- CD14-C159T polymorphism is not associated with familial Mediterranean fever or development of amyloidosis in the population studied
- Signaling by soluble CD14 was TLR2 dependent. CD14-triggered TLR2-mediated response in astrocytes lead to the production of CXCL8, IL-6, and IL12p40, whereas typical TLR-induced pro-inflammatory cytokines were not produced at detectable levels.
- We considered the effects of a common promotor polymorphism of the lipopolysaccharide receptor CD14 associated with increased transcriptional activity upon the development of posttransplant rejection and graft survival.
- CD14-159 and -550 polymorphisms might not play a major role in the pathogenesis of food allergy in Japanese children
- CD14 is a novel ligand for alpha4beta1, exhibiting similar activation-state dependent binding characteristics as other alpha4beta1 ligands.
- reduced signaling through CD14/toll-like receptor 4 in response to challenge by gram-negative bacteria after burns results in a blunted innate immune response and subsequent increased likelihood for systemic infection and death
- Promoter polymorphisms of the CD14 gene did not influence susceptibility to inflammatory periodontitis in the population cohorts studied.
- Reports of progression of atherosclerosis in CD14 gene polymorphism in different populations are conflicting. Screening for CD14 159C/T polymorphism is unlikely to be a useful tool for risk assessment of MI at young age.
- Functional polymorphism in CD14 is associated with greater risk of H. pylori-related gastric carcinoma, which might be mediated by elevated CD14.
- an evident association exists between the carriage of the T-containing genotype and and the extent periodontal disease
- CD14 -550 C/T, which is related to the serum level of sCD14, is associated with the development of respiratory syncytial virus bronchiolitis in the Japanese population.
- nancy will fill
- CD14 polymorphism (-159 cytosine/thymine) might partly explain the difference in predisposition to develop complications of infectious diseases in different patients.
- The existence of a mutation in the CD14 gene is associated with an increased susceptibility to ulcerative colitis.
- In unstable angina patients oxidized LDL may contribute to upregulating CD14 expression in monocytes
- There was no association between IL-10 and CD14 polymorphisms and myocardial infarction occurrence
- functional promoter polymorphism, -260 C > T, of CD14 that is a main receptor of bacterial lipopolysaccharide. the frequencies of CD14 TT genotype was higher in Buerger disease.
- common allelic variants in promoter regions of genes encoding for molecules involved in either the innate (CD14) or adoptive (CTLA-4) immune reactions are associated with an increased risk of GVHD after allogeneic stem cell transplantation.
- Plays a role in mediating monocytic scavenger functions.
- The CD14/-260 and CD14/-651 promoter polymorphisms are associated with atopic hypersensitivity prevalence among young adults exposed to farm environments.
- Subjects carrying both the CD14 (-260) C/C and the LXRbeta (intron 5) G/G genotypes had a six times lower risk of developing AD than subjects without these risk genotypes. These data support a role for innate immune response genes in risk for AD.
- High prevalence of the CD14-159CC genotype in patients infected with SARS is reported.
- the levels of sCD14 and sTLR-2 in saliva were upregulated in oral lichen planus and burning mouth syndrome. Oral epithelial cells in the saliva of patients exhibited elevated levels of CD14 mRNA and decreased levels of TLR-2 mRNA.
- A polymorphism in the CD14 gene (CD14/-1721) modifies the inverse association of early farm milk consumption on allergic diseases in children in 2 independent rural populations, independent of being a farm child.
- CD14 is differently expressed in monocytes in subgroups of acute coronary syndromes
- Transfer of endotoxin from MD-2 to extracellular soluble CD14 reduces activation of cells expressing TLR4 without MD-2.
- The epigenetic control of gene transcription by histone acetylation is important for 1,25D3-regulated cathelicidin and CD14 expression.
- Antioxidants induce a slight increase of differentiated cells and a significant increase of CD14 mRNA in an Acute Promyelocytic Leukemia cell line.
- in the present study, the susceptibility to develop tuberculosis was found to be associated with CD14 -159TT but not with an increase in the expression of CD14
- Dysregulation of TLR2, TLR4, and CD14 expression in different parts of the intestinal mucosa may be crucial in IBD pathogenesis.
- Chlamydiaceae LPS has low binding affinities for LPS recognition molecules such as CD14 and LBP and exhibit weak biological activities against host immune cells including monocytes.
- structural analysis of CD14 and discussion of the role of disulfide bonds and N-linked glycosylation
- 159T allele associated with decreased glomerular filtration rate
- Inflammatory bowel disease appears to be associated with the CD14 c.1-260C>T promoter variant in Germans and Hungarians.
- study has demonstrated a robust augmentation by high glucose of lipopolysaccharide (LPS)-stimulated CD14 antigen expression through activator protein-1 (AP-1) and nuclear factor kappaB (NFkappaB)
- Lipopolysaccharide binding protein and sCD14 are not produced as acute phase proteins in cardiac surgery
- it was concluded that TLR9 and CD14 gene polymorphisms may contribute to an inherited predisposition to asthma in Tunisian children.
- expression in chronic periodontitis lesions is [not] associated with a distinct genotype
- Gene expression analysis of the endothelial-like colonies formed by CD14(+) cells showed that colony formation is a proangiogenic differentiation and might reflect the ability of monocytes to facilitate vascularization
- Increased sialoadhesin expression on CD14(+) monocytes occurs in response to HIV-1 infection with maximum expression associated with high viral load
- In CD14, the rs2569190-TT and rs2569191-CC genotypes associated with lower IgE and decreased risk of sensitisation at 4 and 8 yrs in children exposed to pets, with an opposite effect in nonexposed children
- This study is the first to show higher levels of plasma sCD14 and monocyte mCD14 expression in young, clinically healthy PiZZ AAT subjects.
- These results suggest that LTA signaling preferentially occurs at the plasma membrane, is independent of internalization, and is facilitated by CD36 and CD14 as coreceptors for TLR2.
- Myeloid zinc factor-1 is involved in a calcitriol-induced signaling pathway leading to myeloid cell differentiation and activation of CD11b and CD14.
- It is difficult to establish a role for CD14 in the disease pathogenesis of Dutch sarcoidosis patients.
- intestinal macrophages expressing CD14 contribute to the pathogenesis of Crohn disease via IL-23/IFN-gamma axis
- the monomeric form of sMD-2 is the active species both for reaction with endotoxin.secreted CD14 and Toll-like receptor 4 (TLR4), as needed for potent endotoxin-induced TLR4 activation
- The CD14 gene is very unlikely to be genetically involved in the pathogenesis of Japanese rheumatoid arthritis
- CD14 polymorphisms studied seem not to play a role in determining the severity of alcoholic pancreatitis or the risk of developing alcoholic pancreatitis.
- Patients with graft verus host disease showed increased CD86+Cd14+ cells in peripheral blood and bone marrow cells.
- endothelial mCD14 plays essential role that cannot be replaced by sCD14; Furthermore, we have provided evidence for a TRAM pathway in endothelium that is dependent on mCD14 even when other responses are no longer mCD14 dependent.
- The CD14-260bp T promoter variant in CD14 showed an association with Ankylosing Spondylitis in the Finns only, not patients from the United Kingdom
- Exercise training-induced reductions in CD14+CD16+ monocytes may contribute to the anti-inflammatory effects of exercise training.
- These findings indicated that the polymorphisms of CD14, but not TLR4 Asp299Gly and Thr399Ile mutations, may be an important genetic factor for HBV-related cirrhotic injury in the Chinese Han population.
- the C allele of CD14 & the 4G allele of SERPINE1 were encountered more in house dust mite-allergic asthmatic patients than controls; results indicate PAl-1 & CD14 may interact to affect susceptibility to allergic asthma
- In patients with type 1 diabetes mellitus, the CD14 C-260TT homozygous genotype increases the risk for the development of celiac disease.
- CD14 genotype may modulate the risk of stroke by influencing individual susceptibility and immune response to C pneumoniae infection
- Results suggest that the elevated salivary CD14 concentration may be one of the host-response components associated with the clinical manifestations of periodontal disease.
- single nucleotide polymorphism in the promoter region influences protein expression and confers susceptibility to sepsis
- Our results demonstrate that the +896A/G TLR4 single nucleotide polymorphism (SNP) is associated with Alzheimer disease, whereas no association has been observed with -260C/T CD14 SNP
- There was an interaction between the CD14-159 allele T carrier status and H. pylori antibodies on serum total IgE (P=0.004).
- Carriers of the CD14 gene -159T allele had a marginally increased risk of developing gastric MALT lymphoma than the controls.