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Validated All-in-One™ qPCR Primer for CD9(NM_001769.3) Search again
Product ID:
HQP116282
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29
Gene Description:
CD9 molecule
Target Gene Accession:
NM_001769.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family.
Gene References into function
- peripheral blood monocytes treated for 5--8 days with GM-CSF (i.e. mature Mphi) acquired the capacity to assemble CD9 with alpha3-beta1 integrin, tyrosine phosphatase SHP-1 and with CD46.
- CD9 protein expression was inversely associated with lymph node metastasis.
- By site-directed mutagenesis, CD9 was found to be palmitoylated at any of the four internal juxtamembrane regions.
- C-kit is physically associated with transmembrane 4 superfamily proteins CD9, CD63, and CD81, that may negatively modulate c-kit and thus regulate c-kit receptor sensitivity to SLF in hematopoietic progenitors.
- Infertility of CD9-deficient mouse eggs is reversed by human CD9
- Data show that the expression of the PAINS-13 epitope depends on CD9 association with alpha(6)beta(1) integrin.
- The second extracellular loop sequence (173)LETFTVKSCPDAIKEVFDNK(192) is largely responsible for trasnfected human CD9-mediated CHO cell adhesion to fibronectin and fibronectin pericellular matrix assembly.
- low expression of CD9 may contribute to the highly invasive and metastatic phenotype of small cell lung cancer
- downregulation of CD9 antigen is associated with breast tumor progression
- CD9 expression was rather intensified in gastric cancer tissue in comparison with normal tissues. CD9 expression was more prominent in advanced gastric cancer.
- CD9 does not have a role in progression of human osteosarcoma or predict its prognosis
- CD9 activates the p46 Shc isoform in tumor cells and leads to apoptosis
- cell-surface expression of CD9, a beta1 integrin-associated transmembrane protein that is involved in cell adhesion and cell motility events, was enhanced in the ra-a47 anti-sense oligonucleotide-treated cells.
- The MRP-1/CD9 gene expression was down-regulated in gastric cancer, its expression was probably related to the carcinogenesis and histology types of gastric cancer.
- endothelial CD9 tetraspanins relocalize to the contact site with transmigrating leukocytes and associate laterally with both intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1)
- leukocyte antigen-DR, CD38, and CD9 share a common pathway of tyrosine kinase activation in human monocytes
- tetraspanin intra- and intermolecular interactions are mediated by conserved residues in adjacent, but distinct regions of TM1 and TM2
- phenotypic conversion and reversion of bladder cancer cells is controlled by a glycosynapse 3 microdomain through GM3-mediated interaction of alpha3beta1 integrin with CD9
- results have shown that CD-9 is present in prostate tumour epithelial cells and that it is regulated by androgens in LNCaP-FGC cells
- CD9 is essential for the IL-16-mediated chemotaxis and activation, acting as an IL-16 receptor in HMC-1, a mast cell line that lacks CD4
- A role of CD9 in melanocyte motility was shown.
- results suggest that the restored expression of CD9 in SCLC cells may reduce the metastatic spread of SCLC cells via the inhibition of cell proliferation and motility
- EWI proteins EWI-2 and EWI-F, alpha3beta1 and alpha6beta4 integrins, and protein palmitoylation have contrasting effects on cell surface CD9 organization
- MRP-1/CD9 regulates the actin cytoskeleton by downregulating of the WAVE2, through the Wnt-independent signal pathway.
- CD9 expression by multiple myeloma cells is upregulated in vivo by close interaction of the cells with endothelial cells and CD9 is involved in transendothelial invasion
- CD9 molecules on endothelial cells participate in the mitogenic effect of platelets; results suggest that the tetraspanin CD9 plays an important role in endothelial regeneration
- role of CD81 and CD9 on the cell-to-cell fusion process mediated by HIV-1, syncytia formation induced by HIV-1 envelope proteins and viral entry in human T lymphoblasts
- Ectopic CD9 expression suppressed neurite-like process outgrowth & promoted apoptotic death of some SCLC cells. Its absence in SLCL may contribute to postadhesive morphologic differentiation, survival, & MMP-2 production via PI3K/Akt pathway.
- results clearly point to an involvement of CD9 in the attachment, uptake or processing of adeno-associated virus-2 by target cells
- Expression of CD9 in transitional cell carcinoma bladder is reported.
- CD9 gene inactivation may play an important role in prostate cancer progression.
- transferrin receptor and CD9, CD81, and CD9P-1 are differentially sorted into exosomes after TPA treatment of K562 cells
- Data show that HIV-1 envelope glycoprotein (Env) and core protein (Gag) colocalize strongly with CD63 and CD81 and less strongly with CD9, and suggest that HIV-1 promotes virus assembly and cell-cell transfer by targeting these plasma membrane proteins.
- establish for the first time a role for CD9 in the tumorigenic process
- Nine potential tetraspanin CD9 partners, including claudin-1, were identified.
- CD9 plays roles in cell proliferation and attachment in vitro as well as in in vivo engraftment and that it can be considered as a useful marker to predict the in vivo efficacy of human adipose-derived mesenchymal stem cells.
- examined whether CD63-PI4K55 and CD9-PI4K55 complexes were resident in platelet-lipid rafts, or formed distinct microdomains
- Results demonstrate the physical and functional association of CD9 with epidermal growth factor receptor on MKN-28 cells.
- CD9 expression correlates with disease status and survival of multiple myeloma patients.
- DHHC2 affects palmitoylation, stability, and functions of tetraspanins CD9 and CD151
- Ectopic expression of CD9 in HT1080 cells actually reduced their metastatic ability. CD9 expression reduced lung retention and platelet aggregation activity of the transfectants.
- the role of CD9 in the presentation of TGFalpha in epithelial and carcinoma cells, whose physiology is driven by ligand-induced EGFR activation.
- These data show the high dynamic of interactions in the tetraspanin web and further indicate that the tetraspanin web is distinct from raft microdomains.
- tetraspanins regulate the activity of ADAM10 toward several substrates