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Validated All-in-One™ qPCR Primer for CD4(NM_000616.4) Search again
Product ID:
HQP116017
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD4mut, IMD79, Leu-3, OKT4D, T4
Gene Description:
CD4 molecule
Target Gene Accession:
NM_000616.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- the role of CD4, CXCR4, and CCR5 in HIV envelope-mediated apoptosis was examined in peripheral blood mononuclear cells
- Degradation of the HIV receptor CD4 by the proteasome, mediated by the HIV-1 protein Vpu, is crucial for the release of fully infectious virions.
- Role of CD4 hinge region in GP120 utilization by immunoglobulin domain 1
- cytokine regulation of CD4 expression on monocytes, monocyte-derived macrophages (MDMs), and microglia was investigated
- Results show that the presence of an alpha-helix N-cap in the CD4 cytoplasmic domain increases CD4 affinity to Nef.
- NF-kappaB activation upon interaction of HIV-1 envelope glycoproteins with cell surface CD4 involves IkappaB kinases.
- All of the human testicular macrophages expressed the markers CD45 and MAC387 and most also expressed CD4.
- Ligation of human CD4 interferes with antigen-induced activation of primary T cells derived from CD4 transgenic mice
- CD4 represents a critical turning point that governs the apoptotic and survival programs in T cells, without modifying the physical association with the TCR-CD3 complex.
- CD4 is active as a signaling molecule on the human monocytic cell line Thp-1
- The CD4 coreceptor mediates tolerance-inducing signals when triggered by an appropriate ligand in vivo.
- coreceptor binding domain, the V3 region of the surface envelope (SU) glycoprotein, was replaced by the V3 loop of a CD4- and CXCR4-tropic HIV-1 strain; the resulting virus, termed SIVagm3-X4mc, exclusively used CD4 and CXCR4 for cell entry
- CD4 dimerization is necessary for helper T cell function.
- study of structural and functional homology between human apolipoprotein A-I and envelope proteins of human immunodeficiency virus type 1 in CD4 receptor binding
- Palmitoylation of Cys394 and Cys397 in the CVRC motif of CD4 and CD4's association with Lck are essential for keeping CD4 highly concentrated in lipid rafts.
- evidence that neutrophils from both HIV-1-infected and uninfected donors express endogenous CD4 with conformation similar to that of CD4 expressed on T lymphocytes, and that binds HIV gp120, significantly influencing the biodistribution of HIV
- physical association of CD4 and CCR5 results in receptor cross talk with allosteric CD4-dependent regulation of the binding and signaling properties of CCR5
- HIV-1 entry involves a chemokine-receptor-dependent but CD4-independent entry in neural cells.
- Modulation of NFI-B2 by HIV-1 may represent yet another mechanism by which HIV infection reduces cell surface expression of CD4
- Involvement of the CD4 extracellular domain for the inhibitory effect of oxidative stress on activation-induced CD4 down-regulation in T cells.
- RT-PCR, and related methodologies are not useful substitutes for assessment of CD4 and CD8 cell numbers in HIV-infected persons
- overexpression of CD4 can inhibit T-cell killing by limiting CCR5-using HIV-1 envelope glycoprotein processing and membrane fusion
- CD4 is downregulated by HIV-1 nef
- The degree of human CD4 dependence of the agonist effects of gp120IIIB at the rat CXCR4 receptor is cell-type specific.
- CD4 di-leucine motif is dispensable for nef binding. In contast, this motif is essential for CD4 down modulation.
- examined the folding and solution structures of ternary CD4-Lck-Zn2+ and CD8alpha-Lck-Zn2+ complexes;coreceptor tails and the Lck N-terminus are unstructured in isolation but assemble in the presence of zinc to form compactly folded heterodimeric domains
- downregulated by HIV-1 Nef protein in coorelation with AIDS clinical progression
- Raft localization of CD4 is not required for hiv-1 entry, however, post-binding fusion/entry steps may require lipid raft assembly.
- The number of CD4+CD28- cells in patients after renal transplantation, especially in graft recipients with chronic graft rejection, suggests a role of these cells in chronic graft destruction.
- When undergoing endocytosis, a small fraction of CD4 on the surface membranes of lymphoblastoid cells is targeted to clathrin-coated pits where it becomes associated with CD71 molecules that are also targeted for endocytosis into the same pit.
- downregulated by Hiv-1 Nef protein in association with lipid rafts
- down-regulated by SIVcpz and HIV-1 group N or O nef alleles
- CCR5 HIV coreceptor is significantly more mobile than CD4 and it requires membrane cholesterol for mobility
- We have shown that 3D domain swapping is a likely candidate for the conformational change which influences permissiveness of cells to HIV infection; the hinge loop, or linker, is loop E - F.
- HIV uptake by CD4 T cells requires cellular contacts mediated by the binding of gp120 to CD4 and intact actin cytoskeleton
- entropy calculation of HIV-1 Env gp120, its receptor CD4, and their complex
- CD4 recycling from the plasma membrane and the nascent CD4 in transit to the plasma membrane are susceptible to intracellular retention in HIV-1 Nef-expressing cells
- Deficiency in function of the CD4(+)CD25(+) T-cell population may influence the pathogenesis of type 1 diabetes.
- The T cells exclusively originating from preexisting CD4CD25 regulatory T cells and proves anergic and highly suppressive on isolation.
- The presence of CD4+CD25+ (regulatory T cells in head and neck cancer patients might be, in part, responsible for downregulation of antitumour immune responses.
- CD4(+)CD25(+) fetal T cells are present in the human fetus from week 14 onwards, which shows that generation of regulatory-suppressor T cells is consubstantial to the generation of a functional and self-tolerant immune system
- Cell surface rearrangements in response to CD4 engagement may serve as a means to enhance cell-to-cell signaling at the immunological synapse and modulate chemokine responsiveness, as well as facilitate HIV entry.
- The first is a lag phase that is caused in part by the concentration-dependent reversible association of virus with CD4 and CCR5 to form an equilibrium assemblage of complexes.
- association of CD4+ T lymphocyte nadir with the extent of immune reconstitution in HIV-infected individuals suggests that HIV-1 may cause irreparable immune system damage
- down regulated by four SIVcpz Vpu proteins
- adaptation to microglia correlates with the generation of a gp120 that forms a more stable interaction with CD4
- Nef-induced CD4 down-regulation is not implicated in the Nef/CD4 complex formation in the cellular context.
- Phenotypic studies demonstrated decreased frequency of CD4+CD25+ T cells in patients with chronic graft-versus-host disease
- determination of the structure of V3 in the context of an HIV-1 gp120 core complexed to the CD4 receptor and to the X5 antibody at 3.5 angstrom resolution
- HIV-1 envelope glycoproteins from the brain of an infected patient had a lower CD4 dependence, since they efficiently mediated fusion in the presence of low levels of CD4 on the target cell membrane
- Runx1 blocks the elongation by RNAPII, which may contribute to CD4 silencing during T-cell development.
- We hypothesized that LA might induce dissociation of p56(Lck) from CD4, thus leading to its downmodulation.
- molecular modeling of the global complex of CCR5, gp120 including the V3 loop and CD4
- CD4 and CD25 in T cells, which are increased in breast cancer, are decreased after vaccination with a HER2/neu peptide (E75) and GM-CSF vaccine
- Monomeric forms of CD4 are preferentially used by HIV-1 to gain entry into target cells, thus implying that the dimer/monomer ratio at the cell surface of HIV-1 target cells may modulate the efficiency of HIV-1 entry.
- The results reported in this study demonstrate the activation of both gammadelta- and alphabeta-T cell responses in healthy elderly after influenza vaccination.
- Contribution of Vpu, Env, and Nef to CD4 down-modulation was compared.
- Present and functional on natural killer (NK) cells, CD4 plays a role in innate immune responses as a chemotactic receptor and by increasing cytokine production.
- CD4+ CD45RO+ Foxp3+ CD25hi T lymphocytes are highly proliferative, with a doubling time of 8 days, compared with memory CD4+ CD45RO+ Foxp3- CD25- (24 days).
- This study of tissue-derived HIV-1 Nefs demonstrates that CD4 and MHC-I downregulation are highly conserved Nef functions, while Pak2 association is variable in late stage AIDS patients.
- CD4, complement receptors, and CXCR4 chemokine receptors are required for complement mediated antibody dependent enhanced entry of HIV into MT2 cells.
- The result, including a total of 2640 MS patients and 2194 controls shows no significant association with CD4 and LAG3 and MS. We conclude that these genes are of minor importance in regard of genetic predisposition to the MS.
- human herpesvirus 7 suppresses the replication of CCR5-tropic (R5) HIV-1 in coinfected blocks of lymphoid tissue by downregulation of CD4
- These data support studies using in vitro generated and expanded human CD4+ CD25+ regulatory T cells with indirect allospecificity as therapeutic reagents to promote donor-specific transplantation tolerance.
- The CD4 mRNA expression level strongly correlated with the CD4(+) infiltration score in the cancer epithelium (r(s) = 0.858, P < 0.001) and in the cancer stroma (r(s) = 0.797, P < 0.001).
- In conclusion, numbers of circulating immunosuppressive T(reg) are reduced in transplant recipients.
- CD44 v3-containing isoforms are associated with head and neck squamous cell carcinoma growth, migration, and matrix metalloproteinase activity and can be identified in lymph node metastasis
- results support a model in which HIV-1 Nef downregulates CD4 by promoting its accelerated endocytosis by a clathrin/AP2 pathway
- although CD4+ CD25+ T regulatory cells induced by IL-10 seem to contribute to aspects of sepsis-induced lymphoid immune suppression, the oblation of CD25+ cells does not provide a survival advantage or disadvantage
- results indicate a critical role for Arg(59) in the CD4 for conformational changes in gp120 during the sequential process of entry and infection by HIV-1
- human variant of CD4 modified to be resistant to HIV-1 binding can rescue the signaling for T cell development in the thymus in vivo, having helper T cell functions
- Stimulation of two receptors together, using gp120, a component of HIV enhanced cell adhesion greater than stimulation of CD4 or CXCR4 individually. (gp120)
- CD4 potentiates human iNKT cell activation by engaging CD1d molecules. These results indicate that the CD4 coreceptors may contribute to the fine tuning of iNKT cells reactivity.
- These results suggest that Tregs inhibit osteoclast differentiation from PBMCs in a cytokine-dependent manner, not by cell-to-cell contact manner and that TGF-beta and IL-4 may be the key cytokines for this suppressive function of Tregs.
- CD158a and CD158b display a coactivatory function, involving the c-Jun NH2-terminal protein kinase signaling pathway, when expressed on malignant CD4+ T cells from a patient with Sezary syndrome
- Human CD4(+)CD25(bright) T cells play a major role in tolerating conceptus antigens and therefore may contribute to the maintenance of pregnancy.
- CD4(+)CD25(+)IL-7Ralpha(+) cell population represents a specific marker to monitor allospecific CD4 T cell responses in blood and in tissues after organ transplantation.
- Impaired function of CD4+/CD25+ T regulatory lymphocytes characterizes the self-limited hepatitis A virus infection.
- Results suggest that Siva-1 might participate in the CD4-initiated signaling apoptotic pathway induced by the HIV-1 envelope in T-lymphoid cells.
- CD4+CD25+CTLA-4+FoxP3+ T cells have roles in patients with systemic lupus erythematosus and are impaired after conventional treatments
- These results indicate that CD4 can contribute to natural killer cell activation independently of the presence of a CD4-ligand on antigen presenting cells and suggest that it preferentially modulates cytokine and proliferative responses.
- CD4 interacts constitutively with multiple CCR5 at the plasma membrane of living cells
- CD4+ cells may facilitate the early clearance of bacteria by regulating neutrophils function possibly through an IFN-gamma-dependent mechanism
- we show that L37, P78 and E177 residues of Nef are required for its effect on CD4 internalization and recycling but dispensable for Nef-induced retention and degradation of intracellular CD4.
- antibodies to CD4-induced epitopes may play a role in controlling Simian immunodeficiency virus infection and provide insights for HIV vaccine development
- CD4+CD25highFoxp3+ T cells have a role in squamous cell carcinoma of the head and neck
- TCR-pMHC-CD4 complex in a membrane environment studied as a model for the immunological synapse
- NMR study identified a stable transmembrane helix and a short amphipathic helix in the cytoplasmic region of CD4mut.
- Detection of conformational changes within sCD4 as a result of ligand binding was followed by SAXS on sCD4 bound to two different glycoprotein ligands: the tick saliva immunosuppressor Salp15 and the HIV-1 envelope protein gp120.
- Vaccination-induced HBsAg-specific CD4 T cell responsed can be measured with the use of an interferon-gamma staining assay.
- Strucural basis for the interaction between FAK and CD4 is reported.
- Suggest that CD4+CD25+bright T cells were likely to play anti-inflammatory and immunosuppressive roles in the pathogenesis of primary Sjogren's syndrome.
- Restricted lateral mobility of plasma membrane CD4 impairs HIV-1 envelope glycoprotein mediated fusion
- there is an enrichment of both IL-17+CD4+ and CD8+ T cells in active multiple sclerosis lesions
- Sequence variation occurs within recognized CD4 epitopes during early HIV infection.
- Moderate intensity long-term training may increase the recruitment of active memory CD4+CD25+ in men rather than women.
- The CD4 complex included the following major components: tyrosine phosphatase CD45, transferrin receptor CD71, tyrosine kinase Lck, and a lymphocyte phosphatase-associated phosphoprotein LPAP.
- Campath-1H (Alemtuzumab) is an effective immunodepletion agent used in renal transplantation which increass CD4 regulatory T cells.
- HIV infection of cells with limiting levels of cell surface CCR5 can be facilitated by gp41 sequences
- Increased numbers of CD4+CD25high Tregs may influence IgG4 production in autoimmune pancreatitis.
- gp120/CD4 interactions are the main driving force of the formation of cellular contacts between infected and uninfected CD4 T cells whereby HIV transmission occurs.
- The alloreactivity of expanded cells was negatively correlated with cell expansion and positively correlated with CD4/CD8 ratio and CD8 expression level.
- Results showed that patients with chronic periodontitis presented increased frequency of T lymphocytes and CD4+CD25+ T cells in the inflammatory infiltrate of gingival tissues.
- There was an inverse relation between the CD4 counts and duration of diarrhea from intestinal protozoan infestations in various seasons.
- The kinetics of CD4 antigen T-cell accumulation during a human cutaneous antigen-specific memory response in vivo are reported.
- the genetic polymorphisms at the CD4 enhancer gene are associated with the risk of development of rheumatoid arthritis and systemic lupus erythematosus
- Results suggest that activated moesin promotes F-actin redistribution and CD4-CXCR4 clustering and is also required for efficient X4-tropic HIV-1 infection in permissive lymphocytes.
- The mutation of lysine-297 and arginine-340 on the alpha-adaptin specifically inhibits the ability of Nef to bind AP-2 and downregulate CD4.