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Validated All-in-One™ qPCR Primer for CCNE1(NM_001238.3) Search again
Product ID:
HQP115372
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CCNE, pCCNE1
Gene Description:
cyclin E1
Target Gene Accession:
NM_001238.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle.
Gene References into function
- This study identifies cyclin E1 as a target for activation by ionizing radiation and which plays a functional role in apoptosis of hematopoietic cells. Run-on analyses indicated a predominantly transcriptional regulation of cyclin E.
- TSG101 expression in gynecological tumors: relationship to cyclin D1, cyclin E, p53 and p16 proteins.
- Retinoic acid-induced cell cycle arrest of human myeloid cell lines is associated with sequential down-regulation of c-Myc and cyclin E.
- Proteolytic cleavage of cyclin E leads to inactivation of associated kinase activity and amplification of apoptosis in hematopoietic cells
- found that cyclin A and cyclin E are able to regulate both nuclear and cytoplasmic events because they both shuttle between the nucleus and the cytoplasm
- Cyclin E is a target of WT1 transcriptional repression
- Ectopic expression of cyclin E in estrogen responsive cells abrogates antiestrogen mediated growth arrest.
- The cyclin D1 high and cyclin E high subgroups of breast cancer: separate pathways in tumorogenesis based on pattern of genetic aberrations and inactivation of the pRb node.
- regulates cell cycle
- function and clinical value of cyclin E and p27 in adult acute leukemia (AL)
- CDK-independent transactivation of the estrogen receptor by cyclin D1 is, by itself, not sufficient to cause estradiol-independent growth of breast cancer cells. A vast overexpression of G1/S cyclins D1 A & E is able to do so by capturing CDK inhibitors.
- human cytomegalovirus immediate-early protein 2 can activate cyclin E independent of the cell-cycle state
- A 2.7 A X-ray crystal structure of a Skp1-Cdc4 complex bound to a high-affinity CPD phosphopeptide from human cyclin E reveals a core CPD motif, Leu-Leu-pThr-Pro, bound to an eight-bladed WD40 propeller domain in Cdc4
- increases of cyclin D1, cyclin-dependent kinase 4, cyclin E, cyclin A, and Wee1 play an important role in the development of hepatocellular carcinoma from cirrhosis
- overexpression of cyclin D3 and cyclin E was mutually exclusive possibly reflecting different underlying pathways inducing deregulated expression of these cyclins
- Data suggest that the interaction between PKCeta and cyclin E is carefully regulated, and is correlated with the inactivated form of the cyclin E/Cdk2 complex.
- results show that cyclin D2 is complexed with p27, leading to a model for testicular germ cell tumors whereby the overexpression of cyclin D2 leads to the functional sequestration of p27 in the presence of CCNE and CCND2, favoring cell proliferation
- High levels of this protein are found in malignant glioma.
- Up-regulation of cyclin E is associated with spindle-cell sarcoma
- p220 is an essential downstream component of the cyclin E/Cdk2 signaling pathway and functions to coordinate multiple elements of the G1/S transition.
- CDK2-cyclin E, without prior CDK4-cyclin D activity, can phosphorylate and inactivate pRb, activate E2F, and induce DNA synthesis.
- Assessment of p21, p27, Bax, and cyclin E expression in tumor tissues have been reported to be useful as prognostic factors in head and neck squamous cell carcinoma.
- This review summarizes what is known about the biological role of cyclin E1 and cyclin E2, their deregulation in cancer, and the opportunities they may provide as targets in clinical therapy.
- Cyclin E low molecular weight forms have roles in ovarian tumorigenesis
- The expression patterns of cyclin D1, cyclin E, p21/waf1 and p27/kip1 in inflammatory bowel disease (IBD) may indicate their contribution in epithelial cell turnover and their possible implication in IBD-related dysplasia-carcinoma.
- study shows that cyclin E overproduction is of adverse prognostic significance in adrenocortical tumors
- Cyclin E-mediated impairment of DNA replication provides a potential mechanism for chromosome instability observed as a consequence of cyclin E deregulation.
- Our results demonstrate a physiologic role for TRIP-Br in coupling E2F to novel functions in the regulation of cyclin E expression during cell cycle progression to ensure the proper execution of DNA replication and the maintenance of genomic stability.
- Cyclin E expression is significantly higher in clear cell than in serous ovarian carcinomas. Cyclin E expression is significantly related with p53 positivity.
- We propose that such increased E2F activity stabilizes cyclin E and contributes to establish the high and persistent levels of the protein commonly found in human neoplasias.
- crystal structure of phospho-CDK2 in complex with a truncated cyclin E1 (residues 81-363) at 2.25 A resolution
- overexpressed in breast cancer development.
- low molecular weight forms of cyclin E may contribute to tumorigenesis through their resistance to the inhibitory activities of p21 and p27 while sequestering these CKIs from the full-length cyclin E
- Cyclin E expression may be characteristic for papillary thyroid carcinomas with a tendency to early metastasizing.
- p16(INK4A) reconstitution in p16(INK4A)-deficient T-ALL cells induced cell cycle arrest in the presence of cyclin E and c-Myc expression, uncoupled growth from cell cycle progression and caused a sequential process of growth, differentiation and apoptosi
- The decreased expression of p57(kip2) and/or overexpression of cyclinE protein and PCNA may contribute to the occurrence and progression of pancreatic cancer.
- human CUL4B and cyclin E proteins interact with each other and the CUL4B complexes can polyubiquitinate the CUL4B-associated cyclin E
- These findings suggested that cyclin E may play a role in suppressing the colony formation of CFU-GM by CKbeta8-1.
- Cyclin-dependent kinases regulate the transcriptional activity of FOXM1c; a combination of three phosphorylation sites mediates the Cyclin E and Cyclin A/CDK2 effects.
- Higher cyclin E expression in samples of ZAP 70-positive patients may reflect a larger proliferating compartment in vivo compared to ZAP 70-negative patients with B-cell chronic leukemia.
- The CCNE expression assessment is easy on paraffin-embedded tissue and high CCNE expression hints at the presence of a possible target for individualized cancer therapy.
- The CCNE1 qualify as independent prognostic markers for lymph node-negative breast cancer patients, and CCNE1 may provide additional information for specific subgroups of patients.
- cyclin E expression in breast cancer cells is associated with negative steroid receptor status, HER2 presence, higher tumor grade and higher proliferation index
- an isoform of cyclin E1 involved in G0 maintenance and suggest an additional mechanism for cell cycle control.
- Gene amplification of cyclin E affects the correlation between gene amplification and protein expression of cyclin A, as well as the prognostic value of cyclin A overexpression.
- HuR critically contributes to cyclin E1 overexpression and its growth-promoting function in breast cancer cells.
- This work indicates that-in addition to their function as CDK activators-E cyclins play kinase-independent functions in cell-cycle progression.
- Association of a fragment of cyclin E with Ku70 induces dissociation of Bax, its activation, and apoptosis. These interactions provide a balance between apoptosis and the survival of cells exposed to genotoxic stress.
- cyclin E has a role in dysplasia and multiple pulmonary adenocarcinomas
- Data show that cyclin E overexpression impairs progression through mitosis by inhibiting APC(Cdh1).
- The overexpression of cyclin E positively correlated with the decreasing degree of tumor differentiation, implicating a role for cyclin E in the promotion of tumorigenesis.
- Tissue expression of cyclin D1 or E1 seems not to add independent prognostic value to standard features in patients with nonmuscle -invasive urothelial cell carcinoma of the bladder.
- Cyclin E and SV40 small T antigen cooperate to bypass quiescence and contribute to transformation by activating CDK2 in human fibroblasts
- Immunohistochemical tests for p16 (INK4A) and cyclin E could help in early diagnosis of cervical carcinoma.
- Cyclin E and cyclin D1 expression distinguishes non-BRCA1/2 tumors from both sporadic and BRCA1- and BRCA2-associated tumors and may reflect different predisposition and pathogenesis in these groups.
- Aberrant expression of cyclin E is not associated with low-risk node negative breast cancer.
- Results suggest that cyclin E plays an important, cdk2-independent role in genotoxic stress-induced apoptosis in mesenchymal stem cells.
- Cyclin E is shown to directly interact with and colocalize on centrosomes with the DNA replication factor MCM5 in a centrosomal localization sequence-dependent but Cdk2-independent manner.
- RNA and protein levels of cyclin E1, a dominant oncoprotein, were elevated in the EBP2- enhanced green fluorescent protein stable clones.
- Loss of p27 and overexpression of cyclin E play a critical role in the aggressiveness of gastroenteropancreatic neuroendocrine tumors