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Validated All-in-One™ qPCR Primer for NANOG(NM_024865.3) Search again
Product ID:
HQP112480
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
-
Gene Description:
Nanog homeobox
Target Gene Accession:
NM_024865.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- mouse and human Nanog and Nanog2 genes may have a common role in the maintenance of pluripotency in both species
- Ten processed NANOG pseudogenes are identified in the complete human genome.
- demonstrated changes in DNA methylation in the promoter regions of Nanog and Oct-4 in a human cell line during retinoic acid-induced differentiation
- Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
- NANOG is a new marker for testicular carcinoma in situ and germ cell tumours.
- findings suggest that NANOG acts as a gatekeeper of pluripotency in human embryonic stem and carcinoma cells by preventing their differentiation to extraembryonic endoderm and trophectoderm lineages
- The NANOG protein co-occupy a substantial portion of its target genes, and collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
- We conclude that, despite the difference in primary structure and expression pattern in various stem cells, the alternatively-spliced variant of Nanog has similar activity to that of the full length version.
- NANOG overexpression in human embryonic stem cells enables their propagation for multiple passages during which the cells remain pluripotent
- Nanog possesses an oncogenic potential that may be related to the role it plays in germ cell tumors and to its function in self renewal of ES cells.
- These results suggest that NANOG plays a crucial role in maintaining the pluripotent state of primate embryonic stem cells.
- A series of deletion and site-directed mutagenesis within the homeodomain revealed that two basic NLS motifs are located at the N-terminus and C-terminus of the homeodomain and that both motifs are required for complete hNanog nuclear localization.
- Nanog is described in this review as a unique homeobox transcription factor and key downstream effector of extrinsic signals of embryonic stem cell self-renewal and pluripotency.
- reprogramming of DNA methylation and histone modifications on regulatory regions of the developmentally regulated OCT4 and NANOG genes
- knock-downs of NANOG produced a proliferation rather than a differentiation phenotype in EC cells
- Hence, Nanog does not inhibit terminal differentiation of committed cells but it is an inhibitor of trans-differentiation that is dependent on de-novo activation of gene transcription.
- the CD2 domain of Nanog is a unique transactivator that utilizes aromatic residues to confer specific activity absolutely required for ES self-renewal.
- Sox2-expressing MSCs showed consistent proliferation and osteogenic capability in culture media containing bFGF
- The human primordial germ cells is the first primary cell type described to express POU5F1 and NANOG but not SOX2.
- Sox2, Oct4 and Nanog are linked together in a pluripotent regulatory network
- SMAD 2/3 signaling directly supports NANOG expression, while SMAD 1/5/8 activation moderately represses SOX2.
- may be involved in carcinogenesis of the cervix and progression of cervical carcinoma
- Immunohistochemical localization of nanog in stem cell compartments of human sacrococcygeal teratomas.
- HA binding to tumor cells promotes Nanog protein association with CD44 followed by Nanog activation and the expression of pluripotent stem cell regulators, and forms a complex with Stat-3 in the nucleus leading to Stat-3 and MDR1 activation
- Oct-4 and Nanog may have roles in oral cancer stem-like cells and high-grade oral squamous cell carcinoma
- These findings suggest that NANOG is involved in the pathogenesis and clinical progress of gestational trophoblastic disease, likely through its effect on apoptosis, cell migration, and invasion.
- Identification of the OCT4-pg1 retrogene and NANOG gene expression in the human embryonic eye
- Esrrb coordinates with Nanog and Oct4 to activate the internal machinery of ES cells
- NANOG regulates S-phase entry in human embryonic stem cells (hESCs) via transcriptional regulation of cell cycle regulatory components.