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Validated All-in-One™ qPCR Primer for VIM(NM_003380.4) Search again
Product ID:
HQP111161
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
-
Gene Description:
vimentin
Target Gene Accession:
NM_003380.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM].
Gene References into function
- These findings suggest that platelet vimentin may regulate fibrinolysis in plasma and thrombi by binding platelet-derived Vn.PAI-1 complexes.
- association with MICAL, a novel CasL interacting molecule
- Vimentin expression correlates with the cytoplasmic localization of wild-type p53 in human primary glioblastoma.
- Menin's interaction with glial fibrillary acidic protein and vimentin suggests a role for the intermediate filament network in regulating menin activity.
- Results describe the asymmetric distribution of vimentin in the human sperm head.
- expression correlated with motility of prostate carcinoma cells, poor cell differentiation, and presence of bone metastasis
- specific interaction of the periplakin linker domain with keratin 8 and vimentin
- The 3' untranslated region of human vimentin mRNA binds protein complexes containing eEF-1gamma and HAX-1.
- It is secreted by activated monocyte-derived macrophages
- vimentin expression contributes to the invasive phenotype in androgen-independent prostate cancer but cannot confer it alone.
- The vimentin promoter is a target of the beta-catenin/TCF pathway and strongly suggest an implication of this regulation in epithelial cell migration/invasion in breast cancer.
- role of mitogen-activated protein kinase-activated protein kinase-2 phosphorylation in retaining assembly capacity
- vimentin, in conjunction with low molecular weight heparan sulfate proteoglycans, contributes to the enhanced binding of human group IIA PLA2 to apoptotic T cells
- High MW vimentin was formed after the digestion of vimentin by caspase-3 but not caspase-8. It acted as an autoantigen to form anti-vimentin autoantibody in vivo.
- findings implicate reduced vimentin in the conversion of these tumorigenic prostate epithelial cells into slow growing, less aggressive cells
- study of human vimentin rod 1 structure and the sequencing of assembly steps in intermediate filament formation
- It is unlikely that ependymal vimentin is directly involved in the pathogenesis of Chiari II malformation, but may reflect a secondary upregulation due to defective expression of another gene.
- Data suggest that the PAL-E antibody defines secretion of vimentin as a molecular distinction among endothelial cells and exposes a novel, extracellular role for vimentin in the blood vasculature.
- examination of mutation the LNDR to LNDS motif in vimentin to examine role in epidermolysis bullosa simplex
- Interacts with alpha2beta1-enriched focal adhesions and this association is lost after prolonged adhesion of endothelial cells to collagen.
- Results indicate that protein kinase C epsilon-mediated phosphorylation of vimentin is a key process in integrin traffic through the cell.
- Kidney recipients from non-heart-beating donors showed higher levels of anti-vimentin antibodies than heart-beating donors.
- Apoptotic neutrophils express vimentin on their surface; these cells may participate in the development of autoantibodies directed against cytoskeletal proteins, a condition frequently reported in several inflammatory diseases.
- All polyps were immunohistochemically positive for vimentin.
- regulation of vimentin is controlled by SIP1 in breast neoplasms
- isolated & identified vimentin as the major skeletal-muscle group A streptococcus (GAS)-binding protein; vimentin expression was up-regulated on injured skeletal-muscle cells in vitro and was expressed in muscle tissues from a patient with GAS myonecrosi
- actin and vimentin filaments can interact directly through the tail domain of vimentin
- Data show that the architecture of the vimentin cytoskeleton is modified by perturbation of the GTPase ARF1.
- Increased vimentin expression was found in pseudomyxoma peritonei.
- unit-length filaments appears to be a dynamic and a relatively loosely packed structure with a roughly even mass distribution over its cross-section
- Vimentin is involved in binding of NKp46 to M. tuberculosis H37Ra-infected mononuclear phagocytes.
- The regulatory protease factor Xa is able to cleave IbeA between R297 and K298 residues, and this cleavage abolishes the IbeA-vimentin interaction.
- Vimentin filaments in cross-section exhibit predominantly a four-stranded protofibrilar organization with a right-handed supertwist with a helical pitch of about 96 nm.
- a kinetic model for the in vitro assembly of intermediate filaments from tetrameric vimentin
- Vimentin is a major arterial substrate for transglutaminases.Transglutaminase-mediated vimentin dimerization produces a novel unifying pathway by which vasodilatory and remodeling responses may be regulated.
- In conclusion, ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines.
- The nestin(-) vimentin(+) fibroblasts may represent a novel type of multipotent adult stem cells in human dermis.
- ZBP-89 functions as a repressor by recruiting HDAC1 to the vimentin promoter.
- study of vimentin protein filament structure and assembly by electron paramagnetic resonance spectroscopy of site-directed spin labels. Review.
- The suppression of vimentin expression by ras, and the relief of this suppression by TGFbeta, occurs in a promoter-independent fashion, possibly through sequences in the first or second intron.
- Rults indicate that cysteine 328 in vimention is the main site for prostaglandin PGA(1) addition.
- The vimentin expression patterns in human malignant glioma cells strongly depend on cellular density, algorithms of drug delivery and chemo/radio treatment.
- The interstitial vimentin immunopositivity correlated with sclerotic/fibrotic lesions (interstitial fibrosis, tubular atrophies, vascular hyalinosis/fibrosis, chronicity index), and negatively with glomerular filtration rate.
- Immunohistochemical identification of the combination of calponin and vimentin is suggested for the identification of myoepithelial cells in salivary gland tumors.
- Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways.
- Vimentin isoforms are differentially expressed in normal and transformed astrocytes.
- ability of Surfactant protein A to interact with desmin and vimentin, and to prevent polymerization of desmin monomers, shed light on unexpected and wider biological roles of this collectin
- Affects prostate cancer cells motility and invasiveness.
- coordination of vimentin and detyrosinated microtubules provides structural support for the extensive microtentacles observed in detached tumor cells and a possible mechanism to promote successful metastatic spread
- the O-GlcNAc cycling enzymes associate with kinases and phosphatases at M phase to regulate the posttranslational status of vimentin
- Endosomal lipid accumulation in Niemann-Pick type C1 disease leads to inhibition of protein kinase C, hypophosphorylation of vimentin and Rab9 entrapment.
- vimentin is required for dengue virus serotype 2 infection.
- The role of vimentin in proliferation and motility, was studied.
- These findings suggest that the turnover rate of hepatitis C virus core protein is regulated by cellular vimentin content.
- Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.