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Validated All-in-One™ qPCR Primer for UCP1(NM_021833.4) Search again
Product ID:
HQP111086
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
SLC25A7, UCP
Gene Description:
uncoupling protein 1
Target Gene Accession:
NM_021833.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells.
Gene References into function
- a new polymorphic site disrupts a consensus site for the binding of ATF/CREB transcription factor family and inhibits the factor binding in vitro
- Regulatory motifs for CREB-binding protein and Nfe2l2 transcription factors in the upstream enhancer of the mitochondrial uncoupling protein 1 gene.
- The -3826A > G polymorphism of UCP1 does not play a major role in the development of obesity and/or disturbances of glucose metabolism.
- Data describe the expression of peroxisome proliferator-activated receptor gamma (PPAR gamma) co-activator 1, PPAR gamma, insulin receptor substrate-1, glucose transporter isoform-4, and mitochondrial uncoupling protein-1 in adipose tissue.
- Uncoupling protein 1 polymorphisms are associated with waist-to-hip ratio.
- the polymorphism -3826 A/G of the UCP1 gene is associated with a greater degree of obesity and very high figures of arterial tension.
- GG allele carriers have lowered capacity of thermic effect of a meal in response to fat intake. Impaired UCP1-linked thermogenesis can have adverse effects on regulation of body weight.
- No association between the different polymorphisms and diabetic nephropathy.
- the GG type of the UCP-1 gene has a strong association with increased LDL cholesterol level and might be a significant risk factor for hyper-LDL cholesterolemia among Korean obese subjects
- Polymorphisms in uncoupling protein 1 is associated with Type 2 Diabetes Mellitus
- Our results agree with the previously reported association between UCP1 -3826G allele and obesity and point to a gender-related effect.
- results of the present study provided insufficient evidence of the existence of a major [cleft lip/palate] susceptibility locus in the 4q region
- UCP1 has a new genetic polymorphism, with effects on body fat accumulation.
- UCP-1 polymorphisms have roles in body fat accumulation in Korean female subjects
- Nuclear magnetic resonance study of this protein.
- Ectopic UCP1 gene expression in HepG2 cells affects ATP production.
- These results suggest that insulin resistance caused by the -112C allele influences the susceptibility to type 2 diabetes.
- Our results indicated that young healthy Japanese men with the AG heterozygote of the A-3826G polymorphism in the UCP-1 gene showed higher BMI than those with other genotypes.
- human uncoupling proteins 1 and 2 are activated by polyunsaturated fatty acids in planar lipid bilayers
- Possible involvement of the A-3826G polymorphism in the regulation of body composition.
- the GG genotype of UCP1 may be associated with the presence of hypertension in Japanese males and older subjects
- These results suggest that the GG genotype may be an independent protective factor associated with low HDL-cholesterolemia in this population, although the role of the UCP-1 A-3826G polymorphism in HDL-C is complex and remains controversial.
- The expression of UCP1 mRNA was found in the human epidermis and was upregulated in differentiated keratinocytes.
- UCP1 genetic polymorphisms are associated with blood pressure among Korean female subjects
- no significant associations between these UCP1 genetic polymorphisms and thigh fat areas, visceral fat areas, or blood biochemical profiles, in Korean women