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Validated All-in-One™ qPCR Primer for NKX2-1(NM_001079668.2) Search again
Product ID:
HQP110667
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BCH, BHC, NK-2, NKX2.1, NKX2A, NMTC1, T/EBP, TEBP, TITF1, TTF-1, TTF1
Gene Description:
NK2 homeobox 1
Target Gene Accession:
NM_001079668.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- highly sensitive and specific immunomarker distinguishing between metastatic pulmonary and extrapulmonary adenocarcinoma in effusion cytology specimens
- haploinsufficiency of the TTF1 gene results in a predominantly neurological phenotype and secondary hyperthyrotropinemia
- the association of symptoms in the patients with NKX2-1 mutations points to an important role of human NKX2-1 in the development and function of thyroid, basal ganglia, and lung, as already described for rodents
- TTF-1 and Pax 8 cooperatively activate their target genes and their synergistic activity requires the cross-talk between enhancer and gene promoter
- results show that TTF-1 is not expressed in normal and hyperplastic neuroendocrine(NE) cells or in carcinoids, but is expressed in high-grade NE proliferations and in lung adenocarcinomas
- evidence that mutations in TITF-1 are associated with benign hereditary chorea
- review on use in differentiating neoplasm types
- Data suggest that SMAD3 interactions with the positive regulators NKX2.1 and HNF-3 underlie the molecular basis for TGF-beta-induced repression of surfactant protein B gene transcription.
- the significance of thyroid transcription factor-1 (TTF-1) in the histogenesis of pulmonary sclerosing hemangioma (PSH)
- directly interacts with Pax8 and synergistically activates transcription
- nuclear staining of TTF-1 was observed in two of six adenocarcinoma cell lines, none of seven small cell lung cancer cell lines, and none of three squamous lung cancer cell lines
- -1 is expressed in pulmonary adenocarcinomas and SCLCs
- Glucocorticoid receptor inhibits SP-A promoter activity through the ttf-1 binding element
- Examination of different immunohistochemical markers including TTF-1 is helpful in the diagnosis and differential diagnosis of pulmonary sclerosing hemangioma.
- TTF-1 immunoreactivity favors a diagnosis of primary thyroid medullary carcinoma over laryngeal moderately differentiated neuroendocrine carcinoma.
- NFAT and thyroid transcription factor-1 have roles in regulating transcription of the surfactant protein D gene
- significantly increased the amount of protein-bound radioiodide and prolonged the iodide efflux
- nuclear galectin-3 and TTF-1 have roles in progression of non-small cell lung carcinoma
- Site-specific mutagenesis identifies that lysine-182 in the TTF-1 homeodomain is acetylated in respiratory epithelial cells. Mutation at this acetylation site shows a dominant negative effect on human surfactant protein B gene transcription.
- results demonstrate that interleukin-10 induces uteroglobin related protein 1(UGRP1) gene expression in lung epithelial cells through transcription factor T/EBP/NKX2.1-dependent pathway
- TTF-1 in orbital tissues suggests that it may play an important role in extrathyroidal, as it does in thyroidal, thyrotropin receptor expression
- Presented is a pedigree with infancy-onset benign hereditary chorea (BHC) caused by a novel nonsense mutation in exon 3 (523G-->T, E175X) of the TITF-1 (Nkx2.1) gene.
- a nonsense mutation in the TITF1 gene may be the genetic cause of benign hereditary chorea in a Portuguese family
- pendrin and thyroglobulin are downstream targets in vivo of TTF-1, whose action is a prime factor in controlling thyroid differentiation in vivo
- Thyroid transcription factor 1 gene is not associated with mental retardation in iodine-deficient areas in China.
- PARP-2 and PARP-1 interact with TTF-1 and regulate the expression of surfactant protein B, a protein required for lung function
- p23 forms a specific complex with Hsp90 primarily through binding to its middle domain
- Erm is involved in SP-C regulation, which results from an interaction with TTF-1
- Via promoter mutation analysis, adjacent TTF-1 binding sites within the proximal promoter region of SP-C were found to be essential for TTF-1/TAP26-enhanced SP-C promoter activity.
- 42-kD TTF-1 is required for induction of a subset of regulated genes during type II cell differentiation.
- study supports the fact that TTF-1 could be included in further prospective trials studying prognostic factors in NSCLC
- New syndromic form of benign hereditary chorea is associated with a deletion of TITF-1 and PAX-9 contiguous genes.
- Although morphogenesis of both the pallidum and the hypothalamus requires the presence of transgenic TTF1, only the hypothalamus necessitates the continuous expression of TTF1 to carry out specific differentiated functions.
- GST-FOXA1 fusion protein interferes with the formation of NKX2.1 transcriptional complexes by potentially masking the latter's homeodomain DNA binding function
- calcitonin gene expression could be directly activated by Nkx2.1, whereas Pax9 is not involved in transcription from the 2kbp calcitonin promoter
- Our results suggest that an immunohistochemical panel consisting of TTF-1, CEA, CA-125, and OCT-4 is helpful in distinguishing most pulmonary and ovarian carcinomas with clear cell features.
- TTF-1 expression may be crucial for survival of a subset of adenocarcinomas that express TTF-1, providing credence for the lineage-specific dependency model
- Met3Leu reduced the activity of the RET promoter by 100% in the presence of the wild-type or HSCR-associated RET promoter SNP alleles.
- Anaplastic thyroid carcinoma tissue express TTF1 mRNA abundantly.
- a new splicing mutation (376-2A>C) of the TITF-1 gene was detected in a mother and her daughter in this study.
- We have identified a new mutation in the TTF-1 coding gene in a patient with benign hereditary chorea. The mutation causes a haploinsufficiency of TITF-1 and opens the question of genotype/phenotype correlation.
- Occasional expression of TTF-1 in endometrial and endocervical carcinomas should be kept in mind when evaluating neoplasms of uncertain origin.
- Data suggest that TTF-1 gene missense mutation and synonymous mutation and the loss of TTF-1 protein and mRNA can be regarded as the important indices of molecular pathology of lung carcinoma.
- NKX2-1 mutations could contribute to Hirschsprung's disease by affecting RET expression through defective interactions with other transcription factors.
- TITF1 amplification and overexpression contribute to lung cancer cell proliferation rates and survival
- Data show that direct interaction between Foxp2 and Nkx2.1 inhibits Nkx2.1 DNA-binding and transcriptional activity and suggest a mechanism for down-regulation of SP-C during transition of AT2 cells to an AT1 cell phenotype.
- No mutation or polymorphism was detected in the coding region of thyroid transcription factor-1 (TTF-1) gene in Chinese children with congenital hypothyroidism
- TTF-1 response element is critical for temporal and spatial regulation and necessary for hormonal regulation of human surfactant protein-A2 promoter activity
- Quantitative reverse transcription-polymerase chain reaction (RT-PCR) showed decreased expression of HNF3 beta/FoxA2 and TTF-1 mRNA in papillary thyroid carcinoma cell lines, when compared with normal thyroid cells
- a NKX2.1 mutation may have a role in hypothyroidism and benign hereditary chorea
- a heterozygous TITF1/NKX2.1 mutation leading to neonatal death from respiratory failure.
- The simultaneous positivity for thyroid specific factor TTF-1 and onfFN suggest they might represent putative thyroid cancer stem-like cells.
- TTF-1 was found to be positive in 8 of 48 (16.6%) nephroblastomas and negative in all 5 metanephric adenomas.
- TTF 1 immunostaining has the potential to misguide a pathologist to conclude an ovarian or endometrial tumor being a lung metastasis
- TTF1 expression defines a subgroup of patients with a favorable outcome and may be useful for prognostic stratification of patients with stage I lung adenocarcinoma.
- A missense mutation (1016C>T) was identified in TITF-1/NKX2.1 that led to a mutant TTF-1 protein (A339V) in 4 of the 20 MNG/PTC patients (20%). These patients developed substantially more advanced tumors than MNG/PTC or PTC patients without the mutation