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Validated All-in-One™ qPCR Primer for SOX9(NM_000346.3) Search again
Product ID:
HQP110126
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CMD1, CMPD1, SRA1, SRXX2, SRXY10
Gene Description:
SRY-box transcription factor 9
Target Gene Accession:
NM_000346.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq].
Gene References into function
- Sequence analysis of the intergenic regions revealed five regulatory elements, E1-E5, 5' to SOX9 and three such elements, E6-E8, 3' to SOX9.
- A chromosomally normal boy with ACD and his clinical follow-up up to the age of 2 years, in whom a heterozygous SOX9 missense mutation (H165Y) was identified.
- discussion of molecular action (REVIEW)
- demonstration that a novel human gene, KIAA0800, is preferentially expressed in the testis and is transactivated by Sox9
- novel potential role for SOX9 in pancreas development during human embryogenesis and early foetal life
- RAR agonists stimulate SOX9 gene expression in breast cancer cell lines: evidence for a role in retinoid-mediated growth inhibition.
- mesenchymal chondrosarcoma showed positive nuclear staining in both primitive mesenchymal and cartilaginous components for Sox9 protein
- SOX9 exerts a bifunctional effect on COL2A1 gene expression in chondrocytes depending on the differentiation state.
- CBP and p300 function as co-activators of Sox9 for cartilage tissue-specific gene expression and chondrocyte differentiation.
- Loss of DNA-dependent dimerization of the transcription factor SOX9 as a cause for campomelic dysplasia
- no positive correlation between SOX9 and COL2A1 expression was observed in articular chondrocytes--to the contrary, the expression of COL2A1 was significantly increased in the diseased cells
- SOX9 contributes to growth regulation by mac25 via inhibition of cell growth and promotion of differentiation in prostate tumor
- It is revealed with an in vitro sumoylated Ad4BP/SF-1 that DNA binding activity and interaction with Sox9 ware unaffected.
- Results suggest that SOX9 is necessary and sufficient to specify pyloric sphincter epithelial properties.
- SOX9 is an intestine crypt transcription factor, is regulated by the Wnt pathway, and represses the CDX2 and MUC2 genes
- SOX9 is a key regulator of CRTL1
- Smad3 induces chondrogenesis through the activation of SOX9 via CREB-binding protein/p300 recruitment
- the ubiquitin-proteasome proteolytic system degrades Sox9 and regulates its transcriptional activity
- Transcription factor SOX9 down-regulates CEA gene expression and, as a probable consequence, induces apoptosis in the human colon carcinoma cell line HT29Cl.16E.
- The SOX9, as a potential melanogenic transcriptional regulator, as its expression level is increased following the down-regulation of BRN2 in differentiated melanoblasts.
- Sox9 promoter is regulated by CCAAT-binding factor through its interaction with two functional CCAAT boxes.
- SOX9 may play an important role in the transcriptional activation of the newest collagen gene, COL27A1.
- Sox9 and p300 interact with chromatin and activates transcription via regulation of chromatin modification
- Human SOX9 in undifferentiated mouse ES cells might have dual potentials to induce both chondrogenic commitment and growth capacity in the undifferentiated status.
- by controlling the cellular concentrations of SOX9, PIAS proteins and sumoylation may be part of a major regulatory system of SOX9 functions
- pediatric and adult high grade tumors display strong nuclear staining for SOX9
- SOX9 mRNA regulation in human articular chondrocytes involves p38 MAPK activation and mRNA stabilization
- Sox9 is variably expressed in ovarian Sertoli cell tumor and other tumors that are in the differential diagnosis
- Results indicate that SOX9 in prostate basal cells supports the development and maintenance of the luminal epithelium and that a subset of prostate cancer cells may escape basal cell requirements through SOX9 expression.
- Here we report that the human SOX9 proximal promoter is also regulated by the cyclic-AMP response element binding protein (CREB) and Sp1.
- Sox9 transcriptionally regulates furin expression during chondrogenesis.
- data support a direct molecular link between the Hh signaling pathway and SOX9 regulation, wherein SHH stimulates SOX9 through its mediator GLI1, and are consistent with a mechanism of SOX9 regulation through distal chromatin interactions
- The embryonic male prostaglandin D synthase (Pgds)/SOX9 pathway is expressed at both the RNA and protein levels in different types of human ovarian tumors.
- These findings suggest that non-syndromic PRS may be caused by both SOX9 and KCNJ2 dysregulation.
- SOX9 signaling is not essential for LE135-induced chondrogenesis in mesenchymal stem cells derived from osteoarthritis patients.
- role of SOX9 in pigmentation emphasizes the impact of SOX proteins in adult tissues.
- The expression of genes in a human chondrosarcoma cell line is altered following SOX9 overexpression.
- new hypoxia-inducible and SOX9-regulated genes, Gdf10 and Chm-I. In addition, Mig6 and InhbA were induced by hypoxia, predominantly via HIF-2alpha
- The association of SOX9 hypermethylation with tumour progression and clinical outcome suggests its relevant clinical implications at stratifying patients affected with bladder cancer.
- Critical levels of WT1 + KTS, SRY and SOX9 are required for normal Sertoli cell maturation, and subsequent normal spermatogenesis.
- SOX9 has a role in mediating extracellular matrix deposition characteristic of organ fibrosis
- SOX9 expression is a general feature of basal cell carcinomas and adnexal skin neoplasms, suggesting a contribution of SOX9 to the pathogenesis of these tumors.
- Results support important functions of SOX9 in both the development and maintenance of normal prostate, and indicate that these functions contribute to PCa tumor growth and invasion.
- These findings implicate a mutation at a sex-determining locus other than SRY and SOX9 as the cause for the XX sex reversal trait in this family.
- Data show SOX9 transduction in chondrocytes increased their chondroitin sulfate synthetic capacity, but this was not accompanied by changes in the transcription of the CS biosynthetic enzymes.
- Quantitative changes of enteric glia represented by SOX9 provide a basis for pathological assessment of glial proliferation and/or degeneration in the diseased gut.
- negative regulation of claudin 7 seems to be defective in CRC, possibly due to decreased Sox-9 activity
- Role of the SOX transcription factor family in adult human cartilage is most probably restricted to a few members, with SOX9 being the most prominent.
- SOX9 expression was predictive for favorable outcome in ependymoma
- Sox-9, ER81 and VE-cadherin have roles in retinoic acid-mediated trans-differentiation of breast cancer cells
- Sox9 proteins are exclusively expressed in the cytoplasmic compartment in solid pseudopapillary tumours
- p54nrb plays an important role in the regulation of Sox9 function and the formation of paraspeckle bodies during chondrogenesis
- CEACAM1 is a direct target of SOX9 in the colon epithelium
- SOX-9 is not useful in the chordoma-chondrosarcoma differential diagnosis
- Prostaglandin D synthase-mediated SOX-9 upregulation could provide a reasonable explanation for the presence of testicular differentiation in ovarian ovarian sex cord-stromal tumours.
- study confirmed SOX9 upregulation in colorectal cancer compared with normal mucosa; immunostaining showed more SOX9+ cells in lower zone of colonic crypts than upper zone; cancers with strong SOX9 immunostaining were associated with lower 5-year survival
- expression of Sox1, Sox2 and Sox9 was detected at the mRNA level in both foetal and adult cerebellum samples; Sox1, Sox2 and Sox9 expression was detected in the Purkinje cell layer of the adult cerebellum