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Validated All-in-One™ qPCR Primer for SOX2(NM_003106.3) Search again
Product ID:
HQP110118
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ANOP3, MCOPS3
Gene Description:
SRY-box transcription factor 2
Target Gene Accession:
NM_003106.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq].
Gene References into function
- SOX2 has a role in eye development, and its mutation can cause anophthalmia
- Sox-11 activates transcription more strongly than Sox-2; the transactivation domain of Sox-11 is primarily responsible for this capability
- Sox2 can dimerize onto DNA in a distinct conformational arrangement.
- OCT1 and SOX2 have roles in transcriptional activation of the Oct1.Sox2.Hoxb1-DNA ternary transcription factor complex
- Down-regulation of Sox2 may be an important factor for the development of intestinal metaplasia
- SOX2 may play a role in differentiation of the human gastric epithelium and may be involved in gastric carcinogenesis
- Sox2 may play an important role in maintaining a gastric phenotype in stomach cancers as well as in normal tissue.
- Identification of Sox2 as a novel c-myc BUR-binding protein was achieved through yeast one-hybrid screening and the Sox2/DNA interaction was confirmed by electrophoretic mobility shift assay and immunoprecipitation with Sox2 antibody.
- The SOX2 protein co-occupy a substantial portion of its target genes, and collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
- Bilateral anophthalmia and brain malformations caused by a 20-bp deletion through a slipped-strand mispairing event in the SOX2 gene.
- Expression of SOX2 is involved in later events of pancreaatic carcinogenesis.
- Increased SOX2 is associated with the pancreatobiliary phenotype of ampulla of vater carcinoma and involved in later events in carcinogenesis, such as invasion and metastasis
- This review describes how cross regulation for PAX6, SOX2 and perhaps OTX2 has now been uncovered, pointing to the mechanisms that can fine-tune the expression of three such essential components in eye development.
- SOX2 is necessary for the normal development and function of the hypothalamo-pituitary and reproductive axes in both humans and mice.
- These data suggest that SOX2 plays an important role in regulation of pepsinogen A, and ectopic expression of SOX2 may be associated with abnormal differentiation of colorectal cancer cells.
- SOX2 mutation associated with AEG syndrome.
- Sox-2 immunoreactivity showed a nuclear labeling pattern and colocalised on GFAP immunoreactive cell in the human subventricular zone.
- Sox2 is preferentially expressed in breast tumours with basal-like phenotype.
- Detection of anti-SOX2 T cells predicts favorable clinical outcome in patients with asymptomatic plasmaproliferative disorders
- Sox2 was observed in all cases of immature teratomas in primitive neuroepithelial tissues, but was not expressed in mature tissues.
- RNAi-mediated silencing of SOX2 induced differentiation with mesodermal characteristics in EC cells
- Results provide further evidence that SOX2 haploinsufficiency is a common cause of severe developmental ocular malformations and that background genetic variation determines the varying phenotypes.
- have implicated duplications of SOX3 and mutations of both SOX2 and SOX3 in the aetiology of variants of septo-optic dysplasia
- PAX6 and SOX2 are obvious candidates in RE genetic studies because of their biological roles and prior linkage studies. The present findings strongly suggest refractive error is not directly affected in this population by variants in either gene.
- expression of both SOX2 and MUC5AC in serrated polyps supports the hypothesis that these polyps may be predominant precursors of mucinous and signet ring cell carcinomas of the colorectum
- only SOX2 has been identified as a major causative gene of anophthalima and microphthalmia.
- Using ectopic expression of Oct4, Sox2, Klf4 and Myc, we have derived iPS cells from fetal, neonatal and adult human primary cells
- Sox2 may be a tumor marker of glial lineages
- Sox2-expressing MSCs showed consistent proliferation and osteogenic capability in culture media containing bFGF
- The human primordial germ cells is the first primary cell type described to express POU5F1 and NANOG but not SOX2.
- SOX2 is frequently downregulated in gastric cancers
- SOX2 plays a critical role in the pituitary, forebrain, and eye during human embryonic development.
- A high level of SOX2 expression correlates with epigenetic modifications of distant enhancers SRR1 and SRR2 during creation of cell-specific epigenomes.
- Thsi study identifies DPPA4 and other genes as putative Sox2:Oct-3/4 target genes using a combination of in silico analysis and transcription-based assays.
- These results support the role of SOX2 in ocular development. Loss of SOX2 function results in severe eye malformation. CHX10 was not implicated with microphthalmia/anophthalmia in our patient cohort.
- Sox2, Oct4 and Nanog are linked together in a pluripotent regulatory network
- SMAD 2/3 signaling directly supports NANOG expression, while SMAD 1/5/8 activation moderately represses SOX2.
- SOX2 and beta-catenin act in synergy in the transcription regulation of CCND1 in breast cancer cells
- Mutation in SOX2 is associated with typical ocular coloboma and probably other anomalies in this Chinese family.
- The transcriptional activation of miR-302 and the translational repression of its targets, such as cyclin D1, may provide a link between Oct4/Sox2 and cell cycle regulation in pluripotent cells.
- describe two sisters with bilateral anophthalmia/microphthalmia, brain anomalies and a novel heterozygous SOX2 gene single-base pair nucleotide deletion, c.551delC, which predicts p.Pro184ArgfsX19
- expression of Sox1, Sox2 and Sox9 was detected at the mRNA level in both foetal and adult cerebellum samples; Sox1, Sox2 and Sox9 expression was detected in the Purkinje cell layer of the adult cerebellum