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Validated All-in-One™ qPCR Primer for BMP2(NM_001200.3) Search again
Product ID:
HQP109737
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BDA2, BMP2A, SSFSC, SSFSC1
Gene Description:
bone morphogenetic protein 2
Target Gene Accession:
NM_001200.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq].
Gene References into function
- The expression signals of BMP-2 mRNA in malignant schwannoma were not lower than in benign lesions
- BMP-2 gene transfection tends to accelerate HPDLFs into osteoblast-like cells.
- promotes chondrogenic differentiation of multipotential mesenchymal cells
- Macrophage cell lines produce osteoinductive signals that include bone morphogenetic protein-2.
- BMP-2 and TGF-beta activate both Ras/MAPK/AP-1 and Smad signaling in osteoblasts with Smads modulating AP-1 activity.
- RT-PCR analysis of human bone marrow stromal cells during osteogenesis in vitro: the mRNA levels of bone morphogenetic protein-2 (BMP-2), bone sialoprotein-II (BSP), osteopontin (OP) and cbfa-1 increased with culture time in osteogenic medium.
- REVIEW:Bone morphogenetic protein (BMP)-2 cell cycle arrest and apoptosis in human myeloma cells.
- Local application of recombinant human BMP2 from coated titanium implants significantly improves fracture healing, biomechanical torsional stability, and callus remodeling in a rat model.
- induces osteocalcin expression in osteoblasts
- BMP2 binds to collagen II at a specific binding domain and has a role in embryo development, organogenesis, and regeneration of damaged tissues
- BMP2 has a role in controlling normal and premature cranial ossification in humans [review]
- rhBMP-2 promotes MSC activity and reverses bone and cartilage loss in osteopenic mice
- BMP2 can recruit progenitors to the cementoblastic lineage and regulate differentiation by inducing and enhancing the expression of cementum attachment protein, a cell lineage-specific regulator.
- BMP-2 inhibits PDGF-stimulated proliferation of HASMCs through induction of p21. p21-mediated induction of cell cycle arrest by BMP-2 may initiate osteogenic differentiation of vascular smooth muscle cells, ultimately leading to vascular mineralization.
- BMP2 and BMP4 differently affect activin A induction of erythropoiesis; follistatin and FLRG proteins downmodulate the effects of activin A and BMP2.
- results show that expression of a dominant negative type II TGFbeta receptor impairs BMP2 signaling in MDA-MB-231 breast cancer cells, resulting in an attenuation of the anti-proliferative effects of BMP2 on these cells
- BMP2 exposure can regulate PTEN protein levels by decreasing PTEN's association with the degradative pathway
- In pulmonary artery smooth muscle cells from pulmonary hypertension patients, the BMP-2- or BMP-7-induced apoptosis was significantly inhibited compared with PASMCs from patients with secondary pulmonary hypertension.
- expression of the mature BMP-2 protein is disregulated in the majority of NSCLC. BMP-2 enhancement of tumor cell migration and invasion, as well as stimulating tumor growth in vivo, suggests it has important biological activity in lung carcinomas.
- BMP-2 exerts both positive and negative effects on osteoblasts, possibly depending on the differentiation stage and/or the existing BMP signaling
- Results suggest that rHuIL-11 acts synergistically with rHuBMP-2 to more rapidly stimulate bone formation compared with rHuBMP-2 alone.
- Data show that retinoid-induced expression of bone morphogenetic protein-2 is necessary and sufficient for apoptosis of retinoid-responsive cells.
- Study evaluates several aspects of the osteogenic effect of human BMP-2 protein injected into quadriceps of female C57B1/6J SCID mice.
- In cell culture, IL-1beta and TNF-alpha increased BMP-2 mRNA and protein levels by eightfold and fifteenfold, respectively, whereas IGF-1 and TGF-beta1 had no effect. In cartilage explant cultures, IL-1beta and TNF-alpha increased BMP-2 levels.
- brief exposure to recombinant human bone morphogenetic protein-2 (rhBMP-2)is sufficient to induce irreversible commitment to mineralization in dexamethasone-treated osteoblast cultures
- BMP-2 stimulated Placental growth factor secretion from MG63 osteosarcoma and mesenchymal stem cells
- These results indicate that the 73-92 peptide of bone morphogenetic protein-2 (BMP-2) may be a receptor-binding site and may stimulate bone precursor cells to induce calcification.
- BMP-2 and BMP-6 are expressed in arthritic synovium and are strongly up-regulated by proinflammatory cytokines.
- the effect of MGP on calcification and osteogenic differentiation is determined by availability of BMP-2
- a region on the short arm of Chromosome 20 contains a gene or genes that appear to be a major risk factor for osteoporosis and osteoporotic fractures, including BMP2
- BMP2 acts as a tumor suppressor promoting apoptosis in mature colonic epithelial cells.
- BMP-2 enhanced the angiogenic response in developing tumors
- Decreased BMP2 expression associated with progression to prostate cancer; loss of BMP2 and Smad4 related to progression to a more aggressive phenotype
- BMP2 or 4 in pilomatricoma is responsible for induction of proalpha(1)(II) collagen mRNA in overlying epidermal cells resulting in deposition of type II collagen in dermo-epidermal junction.
- bone morphogenetic protein 2- and transforming growth factor beta-regulated osteoblastic differentiation requires menin, Smads and Runx2
- Myogenin protein stability is decreased by BMP-2 through a mechanism implicating Id1.
- The Bmp2 3' UTR contains essential regulatory elements that act post-transcriptionally
- The expression of tenascin-W is induced by BMP-2 in mammary canacer.
- In the presence of 10 microg rHuBMP-2, muscle-derived mesenchymal cells expressed type II collagen messenger RNA at 4 days after transplantation, and a mature cartilage mass was formed 5 weeks after transplantation in the diffusion chamber.
- BMP-2 upregulates the expression of alphavbeta integrins, and these integrins, in turn, play a critical role in BMP-2 function in osteoblasts
- BMP-2 inhibits DHT-induced growth of LNCaP cells through a decrease in E2F protein expression and suppression of E2F activity by hypophosphorylation of Rb
- (TNF)-alpha induced NF-kappaBeta activation and elicited significant increases in BMP-2 mRNA and protein in human umbilical vein endothelial cells
- To analyze the expression of bone morphogenetic proteins (BMPs) in prostate and breast cancers with established metastasis in bone
- Osterix regulation by recombinant BMP-2 proceeds via protein kinase D and MAP kinase (p38 and JNK) signaling in human mesenchymal stem cells
- BMPs influence the formation of the osteolytic prostate cancer metastases, and treatment modalities that inhibit BMP activity may limit the progression of the lytic component of prostate cancer metastases.
- findings identify functional cross-talk of Id1 expression between Wnt and BMP signaling and demonstrate a novel mechanism for Wnt regulation of the BMP-2 response, linking Id1 expression to Wnt/beta-catenin signaling
- The principal finding of this study was that BMP2 is up-regulated in the chorioamniotic membranes in labor and histologic chorioamnionitis.
- Forced expression of BMP-2 in A549 cells significantly enhanced tumor growth in the lungs following intravenous injection but not of subcutaneous tumors
- data indicate that BMP-2 can utilize a new signal transduction pathway in the NF-kappaB activation system, which plays a crucial role in the survival of the N1511 chondrocytic cell line
- Data show that phosphophoryn (PP) up-regulates bone morphogenetic protein 2 gene expression 12 h post-treatment with PP, much later than initial detection of Smad1 phosphorylation at 30 min.
- identified several BMP-2-responsive genes in LNCaP cells, human prostate cancer cell line
- Bone morphogenetic protein-2 modulates Wnt and frizzled expression and enhances the canonical pathway of Wnt signaling in normal keratinocytes
- autocrine BMP production is necessary for the RUNX2 transcription factor to be active and that BMPs and RUNX2 cooperatively interact to stimulate osteoblast gene expression.
- BMP-2 stimulates Runx2 acetylation
- Finally, two inhibitors of cyclin-dependent kinase 9 (a dominant negative CDK9 and flavopiridol) repressed activity from the MR and BMPR2 promoters
- BMP-2 antagonizes Wnt-3a signaling in osteoblast progenitors by promoting an interaction between Smad1 and Dvl-1 that restricts beta-catenin activation
- Potential use of slow degrading silk fibroin 3-D scaffolds loaded with BMP-2 in combination with human bone marrow cells to induce osteogenesis.
- structure of the ternary complex representing the signaling competent complex of BMP-2 bound to the entire extracellular domains of both its type I receptor, BMPR-Ia, & its type II receptor, ActRII, at a resolution of 2.2 angstroms
- Topical and differential expression of BMP-2/4 and BMP-7 mRNA and protein was found in bursa tissue.
- heparin enhances BMP-2-induced osteoblast differentiation in vitro and in vivo by protecting BMPs from degradation and inhibition by BMP antagonists
- TSG-6 is down-regulated during osteoblastic differentiation, and suppressed osteoblastic differentiation induced by both BMP-2 and osteogenic differentiation medium.
- BMP-2 significantly (>2-fold) upregulated mRNA expression of KCNA5, KCNA7, KCNA10, KCNC3, KCNC4, KCNF1, KCNG3, KCNS1, and KCNS3 but downregulated (at least 2-fold) KCNAB1, KCNA2, KCNG2, and KCNV2 in pulmonary artery smooth muscle
- TNF-alpha increases the stability of BMP-2 mRNA through the pentameric motifs via NF-kappaB
- Bone morphogenetic protein 2 (BMP-2) mRNA was upregulated in injured articular cartilage explants
- GREMLIN 1 was expressed in the stroma of human basal cell carcinoma (BCC) tumors but not in normal skin in vivo. Bone morphogenetic protein (BMP) 2 and 4 are expressed by BCC cells.
- Biglycan is a positive modulator of BMP-2 induced osteoblast differentiation
- Results show calcium-sensing receptor regulate BMP-2 synthesis and secretion required phosphatidylinositol 3-kinase activation.
- the BMP1 prodomain specifically binds and regulates signaling by BMP2 and BMP4
- strong BMP staining is seen in maturing chondrocytes, and thus may play a role in chondrocyte differentiation and/or apoptosis; BMP release by osteoclasts may promote osteoblastic differentiation at sites of bone remodeling
- BMP-2, TGF-beta2, and TGF-beta3 are involved in bone formation in heterotopic ossifications.
- AZFc subdeletions do not seem to cause severe impairment of spermatogenesis in Chilean men.
- Results show that cells contacting 316L steel are exposed to increased concentrations of Ni which suffice to impair BMP-2-induced ALP activity.
- RGMa facilitates the use of ActRIIA by endogenous BMP2 and BMP4 ligands that otherwise prefer signaling via BMPRII and that increased utilization of ActRIIA leads to generation of an enhanced BMP signal
- We conclude that the controlled release of BMP-2 for a certain time period was essential to inducing the osteoinductive potential of BMP-2.
- Increased mRNA expression of BMP-2 in bone marrow derived cultured human mesenchymal stem cells under cyclic tensile strain demonstrates the critical nature of mechanical factors in the osteogenic differentiation process.
- BMP2 augmentation might be critically important in achieving this effect
- BMP-2 induced the chondrogenic differentiation of synovial explants in a dose- and time-dependent manner under serum- and dexamethasone-free conditions
- BMP2 regulates the interaction of IGF-1 with growth plate chondrocytes.
- lysyl hydroxylases and lysyl oxidase/lysyl oxidase-like proteins are differentially responsive to BMP-induced osteoblast differentiation
- Glycidyl methacrylated dextran gelatin scaffolds containing micospheres loaded with BMP2 enhanced periodontal regeneration.
- Approximately half of the prostate tumors displayed increased copy numbers of the BMP2, BMP5, BMP7, and UC28 gene loci, which may account for their abnormal gene expression patterns in neoplastic prostate tissue.
- PKA signaling pathway would be the main intracellular signaling pathway of the EP4 for the anabolic effect of bone and mineral metabolisms
- BMP2 functions via a conserved signaling pathway involving Wnt4 to regulate uterine decidualization.
- Variation in BMP2 masy play a previously unidentified role in aspects of bone density, which may be age- and site-dependent.
- STRO-1 selected rat dental pulp stem cells transfected with adenoviral-mediated human BMP2 gene show enhanced odontogenic differentiation.
- interactive effect on serum ferritin level of rs235756 in BMP2 and a SNP in HJV, with a small additive effect of a SNP in BMP4
- Statins induce apoptosis in colorectal tumor cells through induction of BMP2. Statin therapy may only be effective in SMAD4-expressing tumors and may have adverse effects in SMAD4-negative tumors.
- Cotransfection of mesenchymal stem cells with the combination of the CTLA4Ig and the BMP2 gene stimulates osteoblastic cell differentiation in vitro.
- Members of the transforming growth factor beta (TGFbeta) superfamily, bone morphogenetic protein 2 (BMP2), and growth and differentiation factor 11 (GDF11), can signal cultured RGCs to form dendrites
- polymorphisms in the BMP2 and BMP4 genes, both members of the TGF-beta superfamily, contribute to the susceptibility to otosclerosis and further strengthen the results from the recently reported association of TGFB1 with this disease
- BMP-7 but not BMP-2 and BMP-4 prevents the loss of primitive hematopoietic progenitor cells cultured in SFM plus SF3.
- post-transcriptional mechanisms including regulation of RNA stability, rather than transcriptional mechanisms, contributed to the increased BMP2 expression in mycoplasma-infected cells.
- BMP2 downregulates PTEN via RAS/ERK in a SMAD4-null environment that contributes to cell growth, and constitutes a SMAD4-independent but BMP-responsive signaling pathway.
- HtrA1 may regulate matrix calcification via the inhibition of BMP-2 signaling, modulating osteoblast gene expression, and/or via the degradation of specific matrix proteins
- inactivating BMPR-IA and causing a loss of the BMP-2 tumor suppressor function in colon epithelial cells.
- BMP-2 may promote vascular calcification via increased phosphate uptake and induction of osteogenic phenotype modulation in SMC
- results show that different isoforms of hemojuvlin may play unique physiological roles through defined interactions with distinct signaling proteins such as BMP2 and neogenin, and demonstrate that some of these interactions are defective.
- findings indicate that BMP-2 expression induced by high glucose might be closely related to NF-kappaB activation.
- BMP-2, BMP-4, and BMP-6 are endogenous ligands for Hemojuvelin in hepatoma-derived cell lines, and all 3 of these ligands are expressed in human liver
- Results describe an antiproliferative BMP-2/PPARgamma/apoE axis in human and murine SMCs and its role in pulmonary hypertension.
- BMP-2 Gene is not only a factor associated with the occurrence ofossification of the posterior longitudinal ligament (OPLL), but also a factor related to more extensive OPLL
- These results support a model in which the angular gradient in gap junction-mediated intercellular coupling in the lens, and thus proper lens function, is dependent on signaling between the FGF and BMP pathways.
- BMP-2-producing human adipose tissue-derived mesenchymal stem cells, created using adenoviral gene transfer, induced spinal fusion in athymic rats.
- alleles exerting a beneficial effect in young women may subsequently contribute to phenotypes associated with osteoporosis risk in elderly women
- BMP2 and TGFbeta1 are likely to be involved in HESC decidualization
- BMP-2/4 and BMP-6/7 differentially utilize cell surface receptors to induce osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells
- Overproduction of BMP-2 was noted in ankylosing spondylitits patients but there was an absence of a correlation between BMP-2 and changes in noted in the spine
- In MSI CRC this is associated predominantly with impaired BMPR2 expression and in MSS CRC with impaired SMAD4 expression.
- activation of protein kinase A elicits an immediate response through induction of genes such as ID2 and FosB, followed by sustained secretion of bone-related cytokines such as BMP-2, IGF-1, and IL-11
- The developed synthetic gene is a promising approach for scaling-up the soluble expression of hBMP-2.
- Osteoblast-derived BMP-2 act through Akt and ERK, which in turn activates IKK alpha/beta and NF-kappaB, resulting in the activations of beta1 and beta 3 integrins and contributing the migration of prostate cancer cells.
- Proteomic profiling of CHO cells with enhanced rhBMP2 productivity following co-expression of furin is reported.
- TSG-6 has dual roles in bone remodeling: it inhibits RANKL-induced bone erosion in inflammatory diseases, and its interactions with BMP-2 and RANKL help to balance mineralization by osteoblasts and bone resorption by osteoclasts
- analysis of cis-regulatory elements that control a species-specific difference in the 3'-end processing of BMP2 and potentially of other genes
- These results suggest that the BMP-2 acts through PI3K/Akt, which in turn activates IKKalpha/beta and NF-kappaB, resulting in the activations of beta1 integrin and contributing the migration of human chondrosarcoma cells.
- Our study has provided evidence that BMPR-IB-dependent signaling plays a crucial role in BMP-2 up-regulation of the ALP, OC, Dlx5 and Cbfa1 genes in bone cells, suggesting a role of this receptor in BMP-2-induced osteoblast differentiation in vitro.
- BMP2/4 were selectively expressed by endothelial colony-forming cells but not by early colonies in culture (CFU-Hill).
- The solution structure of BMPR-IA reveals a local disorder-to-order transition upon BMP-2 binding.(
- BMP-2 enhanced the invasiveness of chondrosarcoma cells by increasing MMP-13 expression through the PI3K, Akt, c-Fos/c-Jun and AP-1 signal transduction pathway.
- Chinese Han male patients with TC and CC genotypes in exon 3 (-726) T/C have genetic susceptibility to OPLL but not to more extensive OPLL in the cervical spine.
- Human sclera fibroblasts expressed BMP-2, which promoted cell proliferation, and elicited changes in MMP-2 and TIMP-2, might influence extracellular matrix synthesis.
- BMP-2-containing poly(L-lactic acid) nanofibers affect growth and osteogenic differentiation of human mesenchymal stem cells
- findings show that BMP-2 expression is sufficient for initiating breast cancer microcalcification in a rodent model
- The recruitment of both canonical and noncanonical Wnt pathways is required in BMP-2-mediated angiogenesis.
- plays a crucial role in BMP-2 expression and acetylation of NF-kappaB p65 and p50 subunits by TSA treatment may activate the BMP-2 promoter.
- The effects of BMP-2 on hBMSCs can vary depending on the stage of osteoblast differentiation.