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Validated All-in-One™ qPCR Primer for CCL18(NM_002988.3) Search again
Product ID:
HQP108998
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AMAC-1, AMAC1, CKb7, DC-CK1, DCCK1, MIP-4, PARC, SCYA18
Gene Description:
C-C motif chemokine ligand 18
Target Gene Accession:
NM_002988.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for naive T cells, CD4+ and CD8+ T cells and nonactivated lymphocytes, but not for monocytes or granulocytes.
Gene References into function
- mRNA for the chemokine PARC is expressed in the lower respiratory tract in both healthy subjects and patients with pulmonary sarcoidosis.
- An in vivo role for DC-CK1 is shown in the establishment of primary T cell responses in mice indicating the potential of DC-CK1 as an adjuvant for vaccines against malaria as well as other diseases in which cellular immune responses are important.
- Investigation of the regulation of CCL18 production in maturing dendritic cells proposes that CCL18 may be part of an inhibitory pathway devoted to limiting the generation of specific immune responses at peripheral sites.
- Eosinophil production of PARC, its chemotactic effect on monocytes and lymphocytes, and PARC's previously described localization to the lung suggest that this chemokine might play a role in pulmonary leukocyte trafficking.
- Results suggest that pulmonary and activation-regulated chemokine is a member of the CC chemokine family that acts directly as a profibrotic factor.
- Serum PARC levels thus constitute a novel leukemia marker, possibly reflecting tumor/host cell interactions in the circulation
- Regulation of CCL18 by allergen exposure and microbial products suggests its important role in the initiation and amplification of atopic skin inflammation.
- Conclusive evidence links CCL18 protein expression with various stages of atopic dermatitis and demonstrates the capacity of CCL18 to recruit memory T cells into human skin in a SCID-hu mouse model.
- chitotriosidase and CCL18 have roles in formation of pathological lipid-laden macrophages in type B Niemann-Pick disease
- Native collagen significantly up-regulated CCL18 expression in normal AMs activated with Th2 cytokines via a mechanism mediated by beta2-integrin/ scavenger receptor(s).
- A new function for CCL18--the recruitment of Th2 cells and basophils--suggests that CCL18 may play a predominant role in allergic asthma.
- urinary CCL18 levels are related to Gaucher cell burden in Gaucher patients
- variation in the CCL18-CCL3-CCL4 chemokine gene cluster influences HIV Type 1 transmission and AIDS disease progression
- IL-10 synergistically induces CCL18 secretion in combination with IL-4 of IL-13 on monocytes and monocyte derived cells.
- Differential transcription occurring early in atopic dermatitis skin was indicated for CCL18, CCL13, IFNalpha2, PPARalpha and PPARgamma.
- These findings suggest that CCL18 production by bronchoalveolar lavage cells and serum CCL18 concentrations reflect pulmonary fibrotic activity in patients with idiopathic interstitial pneumonias and those with systemic sclerosis.
- CCl18 is expressed in synovial fluid neutrophils in patients with rheumatoid arthritis.
- Upregulation of CCL18 expression and downregulation of CX3CR1 expression play a role in immune responses against the adult t-cell leukemia cells.
- CCL18 and CCL5 are transiently raised during episodes of UAP, and peak levels of both chemokines are indicative of refractory symptoms.
- CCL18 expression didn't differ in PBMCs, sera and Langerhans cells from intrinsic and extrinsic subgroups, but CCL18 expression was observed in lesional skins and inflammatory dendritic epidermal cells in patients with extrinsic atopic dermatitis.