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Validated All-in-One™ qPCR Primer for ACE2(NM_021804.2) Search again
Product ID:
HQP108537
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ACEH
Gene Description:
angiotensin converting enzyme 2
Target Gene Accession:
NM_021804.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq].
Gene References into function
- role in hydrolyzing biological peptides
- tissue distribution of ACE 2 mRNA by RT-PCR
- We tested the hypothesis that cardiac ACE2 activity contributes to features of ventricular remodeling associated with the renin-angiotensin system
- ACE2 is a functional receptor for the coronavirus that causes severe acute respiratory syndrome (SARS-CoV).
- a metallopeptidase, angiotensin-converting enzyme 2 (ACE2), isolated from SARS coronavirus (SARS-CoV)-permissive Vero E6 cells, that efficiently binds the S1 domain of the SARS-CoV S protein; ACE2 is a functional receptor for SARS-CoV
- ACE2 binds to a 193-amino acid fragment of the SARS coronavirus S protein
- large hinge-bending motion is important for inhibitor binding and catalysis in ACE2
- ACE2 also serves as the cellular entry point for the severe acute respiratory syndrome virus and the enzyme is therefore a prime target for pharmacological intervention on several disease fronts--REVIEW
- ACE2 is a constitutive product of adult-type Leydig cells and may participate in the control of testicular function by as yet unknown mechanisms.
- Angiotensin-converting enzyme 2 gene (ACE2) is little associated in genetic predisposition to hypertension.
- We have examined the kinetics of angiotensin peptide cleavage by full-length human ACE, the separate N- and C-domains of ACE, the homologue of ACE, ACE2, and NEP (neprilysin).
- Failure of angiotensin-(1-9) to increase in response to increased angiotensin I indicated little role for ACE2 in angiotensin I metabolism. Levels of angiotensin-(1-7) were more linked to those of angiotensin I than angiotensin II.
- cloning and characterization of a constitutively secreted form of ACE2
- This article will focus on angiotensin-converting enzyme 2 (ACE2) as a new, potential target of gene therapy for hypertensive disorders.
- Upregulated in nonischemic but not ischemic cardiomyopathy.
- Results identify the SARS coronavirus spike protein binding site on angiotensin-converting enzyme 2.
- A molecular docking model of SARS-CoV S1 protein in complex with human ACE2 was constructed and the interacting residue pairs within this complex model and their contact types are described.
- ADAM17 is the protease responsible for shedding of the SARS-CoV receptor, ACE2
- data identify a critical function for ACE2 in acute lung injury
- report associates severe congenital uropathies and renal hypodysplasia with decreased renin-angiotensin system activity associated with the ACE II genotype and a possible functional imbalance among ATR1 receptors.
- This paper reviews data regarding the biochemistry of angiotensin-(1-7)-forming enzymes and the elucidation of the regulatory mechanisms participating in the expression of ACE2 and angiotensin-(1-7) in the control of the circulation.
- Aortic distensibility was increased by prolonged training in endurance athletes, particularly in those with the ACE II genotype. This effect represents an extracardiac adaptation to chronic prolonged training in athletes.
- ACE2 and ACE are ectoenzymes that have distinct localization and secretion patterns that determine their role on the cell surface in kidney epithelium
- crystal structure at 2.9 angstrom resolution of the receptor-binding domain(RBD)of SARS coronavirus spike protein bound to the peptidase domain of ACE2 shows that the RBD presents a gently concave surface which cradles the N-terminal lobe of the peptidas
- In Finnish type 1 diabetic patients, ACE2 polymorphisms are not associated with diabetic nephropathy or any studied risk factor for this complication.
- The G8790A polymorphism in angiotensin I converting enzyme 2 gene may be related to the essential hypertension with cardiac incompetence in Chinese population.
- ACE2 might be involved in the progression of pulmonary sarcoidosis which may depend on gender.
- ACE2 is expressed in the invading and intravascular trophoblast and in decidual cells during pregnancy.
- ACE2 A/G polymorphism is associated with hypertension in patients with metabolic syndrome
- charged amino acids between residues 22 and 57 were important (K26 and D30, in particular) as determinants on ACE2 critical for SARS-CoV infection
- shown to be localized on the apical plasma membrane of polarized respiratory epithelial cells and to mediate SARS-CoV infection from the apical side of these cells.
- functions as a receptor for hCOV NL63 and 229E S proteins but is engaged diferentially by each strain.
- There was higher angiotensin-converting enzyme (ACE) and chymase mRNA expression and mast cell density in failing than in control myocardium and no changes in ACE2 expression were detected.
- analysis of SARS coronavirus interactions with ACE2
- analysis of spike protein of human coronavirus NL63 interaction with its cellular receptor ACE2
- ACE2 is a receptor for human respiratory coronavirus NL63
- SARS coronavirus airway epithelial infection is associated with ACE2 expression and localization (review)
- Here we show that transgenic mice that express the SARS-CoV receptor (human angiotensin-converting enzyme 2 [hACE2]) in airway and other epithelia develop a rapidly lethal infection after intranasal inoculation with a human strain of the virus.
- ACE2 might play an important role in maintaining a balanced status of local renin-angiotensin system synergistically with ACE by counterregulatory effects confounded by the presence of hypertension
- This review compares the structures, distributions and properties of ACE1 and its new homologue ACE2 in the context of cardiovascular function, focusing on the autocrine/paracrine cardiac and brain renin-angiotensin systems.
- ACE2 is a central enzyme in balancing vasoconstrictor and proliferative actions of Ang II with vasodilatory and antiproliferative effects of Ang-(1-7)
- In this review, ACE2 acts in a counter-regulatory manner to ACE by modulating the balance between vasoconstrictors and vasodilators within the heart and kidney, and may thus play a significant role in the pathophysiology of cardiac and renal disease.
- Enhanced angiotensin converting enzyme 2 regulates the insulin/Akt signalling pathway by blockade of macrophage migration inhibitory factor expression.
- Our data suggest that the polymorphism (A1075G) might be a risk factor-at least a marker-for stage 2 hypertension in males and that the 2 studied polymorphisms might be the indicators of systolic hypertension in females.
- ACE2 overexpression in the THP-1 attenuates AngII-induced MCP-1 production and that this reduction is likely mediated by increased Ang (1-7) level.
- Minor alleles of ACE2 gene might be the genetic modifier for the magnitude of left ventricular hypertrophy in male patients with HCM.
- a decrease in ACE2 may be involved in diabetic kidney disease--REVIEW
- we show that ACE2 circulates in human plasma, but its activity is suppressed by the presence of an endogenous inhibitor
- this study is the first to report co-expression of ACE and ACE2 in human cardiac myofibroblasts
- Data suggest that ACE2 overexpression in the subfornical organ impairs Ang II-mediated pressor and drinking responses at least by inhibiting the Ang II type 1 receptor expression.
- we focus on the recent findings related to the ACE2-angiotensin (1-7)-Mas axis and its putative role as an Angiotensin II-AT(1) receptor counter-regulatory axis within the renin-angiotensin system--REVIEW
- Relationship between urinary mRNA expression of ACE2 and the degree of proteinuria in type 2 diabetic nephropathy.
- ACE-2 protects against lung fibrogenesis by limiting the local accumulation of the profibrotic peptide ANG II.
- the major species barriers are determined by interactions between four ACE2 residues (residues 31, 35, 38, and 353) and two RBD residues (residues 479 and 487)
- The spike protein of SARS-CoV (SARS-S) induced TNF-alpha-converting enzyme (TACE)-dependent shedding of the ACE2 ectodomain.
- study identifies smooth muscle cell alpha actin positive ACE2 and AT4R in blood vessels as well as in angiogenic vessels, indicating a possible role for these enzymes in pathological disease
- The authors demonstrated that the receptor-binding domain (RBD) of the spike protein alone could be internalized into SARS-CoV susceptible cells together with ACE2.
- Genetic variation in angiotensin-converting enzyme 2 gene is associated with extent of left ventricular hypertrophy in hypertrophic cardiomyopathy.
- Angiotensin converting enzyme-2 confers endothelial protection and attenuates atherosclerosis.
- We found that ACE2 of Caco-2 cells co-purifies with marker proteins of detergent-resistant membranes
- Found that the A1075 allele of the ACE2 gene was independently associated with higher mortality in an acute coronary syndrome cohort of males of predominantly European ancestry.
- A lower-affinity interaction with ACE-2 may partly explain the different pathological consequences of infection by SARS-Coronavirus and NL63 coronavirus.
- ACE II genotype is identified as a valuable risk factor in the development and outcome of acute kidney injury.
- Amino acid residues important for the chloride sensitivity, substrate selectivity and inhibition of testicular ACE and ACE2 were identified by site-directed mutagenesis.
- kidney disease of patients with type 2 diabetes is associated with a reduction in ACE2 gene
- Polymorphisms of the angiotensin-converting enzyme gene and endothelial nitric oxide synthase gene have been suggested to be associated with left ventricular hypertrophy.