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Validated All-in-One™ qPCR Primer for PKM(NM_002654.5) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
Gene References into function
- measurement of fecal Tumor M2-PK concentrations might provide an interesting screening tool for colorectal cancer
- With the fuzzy logic method and a tumor marker panel (including the new marker Tumor M2-PK), a useful diagnostic tool for the detection of progression in lung cancer patients is available
- phosphorylated at tyrosine residues in breast cancer patients; did not find any evidence that this phosphorylation is initiated by the oncoprotein HER-2/neu
- The X-ray structure of tumor specific PKM2 complexed with Mg2+, K+, inhibitor oxalate, and allosteric activator fructose 1,6-bisphosphate has been determined, and its unique structural characteristics relative to other known isoforms have been analyzed.
- PanK4 interacts with Pkm2 and thereby may modulate the glucose metabolism through regulating the activity of Pkm2.
- PK can function as an autoimmune target and that this immunoreactivity may be associated with Tourette syndrome, obsessive compulsive disorder, and associated disorders
- A sensitive and specific tumor marker for colorectal cancer, seen in older adults.
- An elevated level in this enzyme on admission in suspected periampullary cancer is a predictor of adverse prognosis in periampullary cancer.
- PKM2 exists in either an active tetrameric form which has high affinity for its substrate phosphoenolpyruvate (PEP) or a less active dimeric form which has low affinity for its substrate.
- results indicate that expression of this phosphotyrosine-binding form of pyruvate kinase is critical for rapid growth in cancer cells
- expression of pyruvate kinase M2 isoenzyme was found in 53% 0f granulosa cell tumors of the ovary
- aberrant reduction of miR-133a and miR-133b was associated with the dysregulation of M2-type pyruvate kinase (PKM2) in squamous cell carcinoma (SCC) of the tongue.
- NS5B interacted with M2 type pyruvate kinase but not L type pyruvate kinase.
- Data confirmed the cancer-associated expression of the PKM2 variant with primary colon cancer and colon cancer cell lines, with PKM2 expressed at high levels in colon cancer and in colon cancer cell lines.
- fecal pyruvate kinase M2 is an inferior biological marker of inflammatory bowel disease when compared with fecal calprotectin