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Validated All-in-One™ qPCR Primer for YBX1(NM_004559.4) Search again
Product ID:
HQP102987
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BP-8, CBF-A, CSDA2, CSDB, DBPB, EFI-A, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1
Gene Description:
Y-box binding protein 1
Target Gene Accession:
NM_004559.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- interacts with an upstream element in the alpha1(I) collagen gene
- YB-1 relocates to the nucleus in adenovirus-infected cells and facilitates viral replication
- NMR structure and DNA-binding properties of the cold-shock domain of the human Y-box protein YB-1
- interacts with JCV agnoprotein to modulate transcriptional activity of JCV promoters
- data suggest that p73 stimulates the transcription of the YB-1 promoter by enhancing recruitment of the c-Myc-Max complex to the E-box
- The central and C-terminal domains of YB-1 are required for specific binding to 8-oxoguanine-containing RNA, a mechanism that involves sequestering oxidatively damaged RNA from normal processes and contributes to the high fidelity of translation in cells
- Nuclear expression of YB-1 protein correlates with P-glycoprotein expression in human breast carcinoma.
- nuclear YB-1 shuttling and alternative splice site selection is regulated by SRp30c interaction with YB-1
- YB-1 functions under the regulation of GATA factors in erythroid differentiation and aberrant expression of YB-1 gene may result in dyserythropoiesis
- YB-1 is a cell cycle stage-specific transcription factor important for cell proliferation
- Nuclear localization of this protein requires wild-type p53.
- YB1 interacts directly with Smad3 during TGFbeta signal inhibition by interferon gamma
- findings suggested that YB-1 functions in the early stage of erythropoiesis and that aberrant expression of this protein may induce hematological diseases such as MDS
- YB-1 is involved in base excision and mismatch repair pathways
- autoregulation of YB-1 mRNA translation via the 5'-UTR
- Hepatic nuclear factor 3 and nuclear factor 1 regulate 5-aminolevulinate synthase gene expression and are involved in insulin repression
- 5-HTT VNTR domains differentially respond to YB-1 which binds to the VNTR in a sequence-specific manner-- a new mechanism explaining the ability of the distinct VNTR copy numbers to support differential reporter expression based on YB-1 binding sites.
- study demonstrates that NF-1 plays an important role in PMA/IL-1-mediated transactivation of the JC virus early promoter; suggests a possible involvement of NF-1 in JC virus reactivation in immunosuppressive states such as AIDS
- YB-1 mediates DNA polymerase alpha gene expression
- Overexpression of a transfected gene encoding YB-1 in human HeLa cervical carcinoma cells significantly represses the transactivation of a minimal AP-1 reporter construct in response to the tumour promoter PMA.
- YB-1 overexpression can induce EGF independence in human mammary epithelial cells via activation of the EGFR pathway
- activated Akt is positively correlated with the protein expression of the transcription/translation factor Y-box binding protein-1 (YB-1) in primary breast cancer; activated Akt binds to and phosphorylates the YB-1 cold shock domain at Ser102
- The release of YB-1 from promoter DNA coupled with its ability to bind RNA and shuttle between the nucleus and cytoplasm is suggestive of a regulatory loop for coordinating SMalphaA gene output.
- YB-1 expression is correlated with PCNA expression in NSCLC. The DNA repair pathway and tumor proliferation mediated by YB-1 linking to PCNA may be responsible for controlling the growth of NSCLC.
- FRGY2a and YB1 disassemble nucleoli by sequestering B23, which is associated with pre-ribosomes and other structurally important nucleolar components
- NSEP1 is an authentic selenocysteine insertion sequence binding protein that is structurally associated with the selenoprotein translation complex and functionally involved in the translation of selenoproteins in mammalian cells
- YB-1 colocalized with P-body (messenger RNA processing bodies)
- conclusion, the data demonstrate the utility of the analyzed RNAs as powerful laboratory tools and indicate that YB-1 is not involved in the regulation of the MDR1 gene.
- YB-1 nuclear expression is an important prognostic factor in ovarian carcinoma.
- PP2Cgamma modulates alternative splicing of specific pre-mRNAs coregulated by YB-1.
- OSU-03012 prevents YBX1 from inducing EGFR.
- YB-1 thus appears to have a novel regulatory role in NEIL2-mediated repair under oxidative stress.
- Y-box binding protein-1 (YB-1) as a new repressor of MMP-13 transactivation.
- Radiation mediated increase of nuclear YB-1 in glioma cells enhanced the oncolytic potential of adenovirus dl520
- TGF-beta1 translation in proximal tubule cells requires YB-1 binding to a high-affinity site in the 5'-untranslated region of its mRNA.
- Both YB-1 and Twist expression could induce tumor progression, promoting cell growth and driving oncogenesis in various cancers.
- that the recruitment of YB1, PURalpha, and H1.2 to the p53 target gene Bax is required for repression of p53-induced transcription.
- YB-1 binds specifically to the auto-inhibitory domain of hNTH1, providing a mechanism by which YB-1 stimulates hNTH1 activity.
- HER2 and estrogen receptor alpha expression depends upon nuclear localization of Y-box binding protein-1 in human breast cancers
- YB-1 nuclear expression support the concept that alveolar and embryonal rhabdomyosarcomas are molecular biologically distinct neoplasms.
- Results indicate that the splicing and the nuclease activities associated with YB-1 have minor impact on cisplatin resistance.
- Among three possible YB-1 binding sites in the CCL5 promoter, a critical element was mapped at -28/-10 bps. This site allowed up-regulation of CCL5 transcription in human monocytes; however, it repressed transcription in differentiated macrophages
- APE1 downregulation sensitizes MDR1-overexpressing tumor cells to cisplatin or doxorubicin, showing APE1's critical role in YB-1-mediated gene expression and drug resistance in tumor cells.
- The authors showed that RBBP6 and its isolated RING finger domain were able to ubiquitinate YB-1, resulting in its degradation in the proteosome. As a result, RBBP6 was able to suppress the levels of YB-1 in vivo and reduce its transactivational ability.