|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for NCAM1(NM_001076682.3) Search again
Product ID:
HQP102756
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD56, MSK39, NCAM
Gene Description:
neural cell adhesion molecule 1
Target Gene Accession:
NM_001076682.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Gene References into function
- NCAM 105-115 kDa is a protease- and neuraminidase-susceptible fragment and was correlated with ventricular enlargement in chronic schizophrenia (p = 0.01). Release of NCAM fragments in schizophrenia may be part of the pathogenic mechanism.
- Significance of cell adhesion molecules, CD56/NCAM in particular, in human tumor growth and spreading.
- exists in lipid microdomains and transducts cell signalings via regulating the activation of signal transduction molecules
- PSA-NCAM was found to be expressed in the somata, dendrites and axonal processes of some neurons, which were identified as chandelier cell axon terminals (chandelier terminals), in the adult human entorhinal cortex and neocortex.
- CD56 expression predicts occurrence of CNS disease in acute lymphoblastic leukemia but not CR or survival. CD56 may enable targeting of leukemic cells to tissues that express it.
- CD56 molecules on NK cells interact with fibroblast growth factor receptor 1 on Jurkat T cells to trigger IL-2 production.
- Preferential apoptosis of CD56dim natural killer cell subset in patients with cancer.
- a useful immunohistochemical marker of Merkel cell carcinoma
- which the polysialyltransferases bind to the first fibronectin type III repeat (FN1) of NCAM to polymerize polysialic acid chains on appropriately presented glycans in adjacent regions.
- Strong overexpression of NCAM(CD56) and RUNX1(AML1) is a constant and characteristic feature of cardiomyocytes within or adjacent to scars in ICM.
- Natural killer (NK) cells expressing high CD56 levels are terminally differentiated cells identical to mature NK cells recently activated in the presence of IL-12, and not a functionally distinct subset or progenitors to mature CD56+low NK cells.
- there are two types of CD56+ epithelial cells in the pancreatic duct system: CD56+ endocrine cells are numerous during the early stage of gestation while CD56+ luminal cells may represent developmental and regenerative changes of pancreatic ducts.
- CD56 is expressed in bone marrow of acute myelogenous leukemias but not acute lymphoblastic leukemia
- Alzheimer patients presented values of low molecular weight-NCAM and high molecular weight-NCAM significantly higher than healthy controls of similar age (higher than 130 kDa)
- genetic variations in neural cell adhesion molecule 1 or nearby genes could confer risks associated with bipolar affective disorder in Japanese individuals.
- REVIEW: Prognostic significance of CD56 expressed in multiple myeloma
- CD56 expression was associated with the leukemogenetic mutation at the primitive hematopoietic progenitor cell level
- Unlike wild-type FGFR4, pituitary tumor derived-FGFR4 does not associate with neural cell-adhesion molecule (NCAM).
- Cells expressing this antigen repond to a Wilms tumor cell line feeder cells and are precursors of NK cells.
- Pre-activated, adherent-natural killer cells express low levels of CD56 and CD161
- There is a close relationship between PSA-NCAM expression and neuronal migration.
- CD56bright natural killer cells accumulate in inflammatory lesions and, in the appropriate cytokine environment, can engage with CD14+ monocytes in a reciprocal activatory fashion, thereby amplifying the inflammatory response.
- first pediatric case describing coexpression of CD56 on B-lineage acute lymphoblastic leukemia
- CD56 seems to be the most sensitive marker for the diagnosis of SCC of the uterine cervix
- A significant increase was observed in PSA-NCAM, NCAM-180, NCAM-140, and HSP70 expression as seen by Western blotting and immunocytofluorescent studies in NMDA-treated cultures.
- polysialyltransferase ST8Sia IV/PST recognizes specific amino acids in the first fibronectin type III repeat of the neural cell adhesion molecule
- analysis of polymerization of polysialic acid on neural cell adhesion molecules
- NCAM is associated not only with a cell-to-cell adhesion mechanism, but also with tumorigenesis, including growth, development and perineural invasion in human salivary gland tumors
- CD56(bright) and CD56(dim) human natural killer-cell subsets exert different functional and cytotoxic activities in response to a live bacterial pathogen.
- The new role of Neural Cell Adhesion Molecules in tumor neo-angiogenesis relevant for endothelial cell organization into capillary-like structures.
- NCAM is hyposialylated in hereditary inclusion body myopathy skeletal) muscle.
- Tests whether activation and expansion of human NK cells with lipopolysaccharide (LPS) reveals differences between identical twins with regaard to C56 antigen.
- The gene expression profile of hepatic stem cells throughout life consists of high levels of expression of neuronal cell adhesion molecule (NCAM).
- identified co-induction of NKG2A and CD56 on activation of TH2 cells
- Isolation of 3 novel isoforms of AML 1 (RUNX1) with different transactivating function, that might be a regulatory element of the NCAM (CD56) overexpression in chronic myocardial ischemia.
- the FN1 alpha-helix is involved in an Ig5-FN1 interaction that is critical for the correct positioning of Ig5 N-glycans for polysialylation
- Co-immunoprecipitation and co-clustering paradigms were used to show that both NCAM and N-cadherin can interact with the 3Ig IIIC isoform of the FGFR1 in a number of cell types.
- NCAM1 along with RUNX1 is overexpressed during stress hematopoiesis in Down syndrome children and may contribute to the development of overt leukemia.
- In the "Europe only" stratum, there were nominally significant associations with five contiguous SNPs.
- Neuroblastoma cells resistant to anticancer drugs have increased invasive capacity caused by down-regulation of NCAM adhesion receptor.
- GPI-anchored NCAM-120 suppressed rabies virus replication via induction of IFN-ss even though NCAM-120 was able to promote virus penetration into the cells.
- The pattern of serum NCAM bands could be useful to detect brain tumor pathology. NCAM immunostaining of tumors was inversely correlated with the histological grade of malignancy. Loss of NCAM staining was significantly associated with poor prognosis.
- Renal NCAM-expressing interstitial cells can participate in the initial phase of interstitial fibrosis.
- Single nucleotide polymorphisms within NCAM1 contribute differential risk for both bipolar disorder and schizophrenia possibly by alternative splicing of the gene.
- HIV-1 Nef protein up-regulates the ability of dendritic cellss to stimulate the immunoregulatory naturak killer cells.
- PSA-NCAM is expressed in the human PFC neuropil following a laminated pattern and in a subpopulation of mature neurons, which lack doublecortin expression. Most of these cells have been identified as interneurons expressing calbindin
- Although CD56 expression level varies among the cases, this molecule might play some roles in the manner of growth and expansion of CD56-positive B-cell lymphomas
- PSA-NCAM is not involved in masking Siglec-7
- association studies of alcohol dependence and 43 SNPs mapped to the gene cluster of NCAM1, TTC12, ANKK1 and DRD2
- may explain the preferential accumulation of CD56(bright) NK cells often seen in environments rich in reactive oxygen species, such as at sites of chronic inflammation and in tumors
- lack of CD56 expression on MM cells is not a prognostic marker in patients treated with high-dose chemotherapy, but is associated with t(11;14).
- fetal forebrain axonal PSA-NCAM expression is inversely related to primary myelination
- that expansion of the CD56(bright) NK cell subtype in peripheral blood is not a hallmark of TAP deficiency, but can be found in other diseases as well
- Ubiquitylation represents an endocytosis signal for NCAM.
- NCAM expression may be used as a predictor of perineural invasion in adenoid cystic carcinoma
- Monitoring of T cells driven to senescence showed de novo induction of CD56, the prototypic receptor of NK cells.
- Ganglioneuromas and ganglioneuroblastomas express the adhesive 120 kDa NCAM isoform, while neuroblastomas preferentially express the 180 kDa isoform classically involved in cell motility
- determined the clinical characteristics of 204 multiple myeloma (MM) patients and 26 plasma cell leukemia (PCL) patients with regard to CD56 expression.
- NCAM-fibroblast growth factor receptor 1 interaction at the cell surface is likely to depend upon avidity effects due to receptor clustering
- Neural cell adhesion molecule-extracellular domain overexpression disrupts aborization of basket cells during the major period of axon/dendrite growth in transgenic mice.
- identified molecular characteristics of an aggressive subset of pediatric patients with AML through a prospective evaluation of CD56+ neural cell adhesion molecule (NCAM) and CD94 expression
- relative frequency of CD19 and/or CD56 expression in acute myeloid leukemia (AML) with t(8;21) was significantly higher than those without this translocation and co-expression of these two antigens may serve as the surrogate markers for AML with t(8;21)
- direct receptor-receptor interactions are not required for high affinity GDNF binding to NCAM but play an important role in the regulation of NCAM-mediated cell adhesion by GFRalpha1
- results provide evidence that the BCL motif is one of the multiple FGFR binding sites in NCAM
- Enzymatic removal of PSA from NCAM or reduction of polysialyltransferase expression led to reduced association between NCAM and E-cadherin and subsequently increased E-cadherin-mediated cell-cell aggregation and reduced cell migration.
- CD56 is often expressed by a wide variety of spindle cell sarcomas, thus, it has no value in differentiating GYN from non-GYN spindle cell tumors.
- there was increased placental expression of NCAM (neural cell adhesion molecule) in small for gestational age cases
- extramedullary relapse of MM is characterized by loss of CD56 expression
- CD56-expressing gammadelta T lymphocytes are resistant to Fas ligand and chemically induced apoptosis
- No genetic association between NCAM1 gene polymorphisms and schizophrenia in the Chinese population.
- Immunological events associated with the luteinizing hormone surge induce alterations in all subsets of CD56(+) cells in the fertile menstrual cycle
- CD56(bright) NK cells postallogeneic hematopoietic stem cell transplantation exhibit peculiar phenotypic and functional properties
- To identify the gene regulated by PKD2 and c-Myc, we performed gene expression profiling in PKD2 and c-Myc overexpressing cells. NCAM is an important molecule in the cystogenesis induced by PKD2 overexpression.
- NCAM represents a marker for neuroblastomas irrespectively of their stages
- The results obtained show that, in man, the expression of PSA-NCAM in selective populations of central and peripheral neurons occurs not only during prenatal life, but also in adulthood.