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Validated All-in-One™ qPCR Primer for MMP7(NM_002423.4) Search again
Product ID:
HQP102434
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
MMP-7, MPSL1, PUMP-1
Gene Description:
matrix metallopeptidase 7
Target Gene Accession:
NM_002423.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq].
Gene References into function
- MMP-7 activity is upregulated in colorectal cancer liver metastases
- plays an important role not only in tumor metastasis but in micrometastasis to lymph node
- Overexpression of MMP-7 is associated with invasiveness in gastric carcinoma
- Gene expression analysis reveals matrilysin as a key regulator of pulmonary fibrosis in mice and humans.
- Immunoelectron microscopy revealed that matrix metalloproteinase-7 was expressed within basal laminar deposits and amorphous materials around the retinal pigment epithelial cells in age-related macular degeneration.
- destruction of the surrounding matrix by endometriosis might be caused by various MMPs, which are mainly produced in stromal cells.
- study of expression in ulcerative colitis related tumorigenesis
- Cleavage of FasL by MMP-7 occurs at the leucine residues in sequence "ELAELR" within the region between the transmembrane and trimerization domains. When this site is unavailable, a "SL," is cleaved. MMP-7 differentially processes murine and human FasL
- increased expression of matrix metalloproteinase-7 is associated with high grade transitional cell carcinoma of the urinary bladder may be associated with tumor invasion and metastasis
- Increased expression of MMP-7 in high grade renal cell carcinoma might be associated with tumor invasion and metastasis
- In alveolar-like epithelial cells, transfection of activated matrilysin resulted in shedding of E-cadherin and accelerated cell migration. In vivo, matrilysin co-localized with E-cadherin at the basolateral surfaces of migrating tracheal epithelium.
- findings suggest that local pericellular production of hypochlorous acid by phagocytes is a physiological mechanism for governing matrix metalloproteinase-7 activity during inflammation
- MMP-7 is induced in human gastric epithelial cells infected with Helicobacter pylori; it has a role in epithelial cell migration
- src oncogene signaling involves synergistic cooperation between the AP-1 and LEF-1 transcription factors in activation of the matrilysin promoter in colon cancer cells.
- Data show that matrilysin confers macrophages with their most potent matrix metalloproteinase-dependent elastolytic system.
- Down-regulated PTEN expression and up-regulated MMP-7 expression were greatly implicated in tumorigenesis and progression of gastric carcinoma.
- May contribute to the tumorigenesis of MMP-7-producing IGF-IR-expressing tumors in the primary site and to organ-specific metastasis in a paracrine manner
- Heparanase, CD44v6 and nm23 may play important roles in the invasive infiltration and lymph node metastasis in gastric carcinomas.
- matrix metalloproteinase-7 has a role in progression of pancreatic cancer
- matrilysin may have a role in renal tubular injury and progression of tubulointerstitial fibrosis, and Wnt4 may regulate matrilysin expression in the kidney
- the importance of MMP7 as a predictor of secondary Extramammary Paget's disease or the putative origin of Paget's cells from the dermal adenocarcinoma cells of apocrine duct origin
- Overexpression of MMP-7 is associated with non-small cell lung cancer
- These results indicate MMP-7 is overexpressed in malignant ovarian epithelium and suggest MMP-7 may facilitate tumor cell invasion in vivo partly through the induction of progelatinase activation.
- alcohols are considered to bind the hydrophobic S1' subsite most plausibly, and the size of the pocket was estimated to be large enough to accommodate the length of 1-butanol (4-carbon chain) and the bulk of tertiary alcohols
- pattern of secretion in polarized MDCK cells expressing stably transfected human MMP-7
- Results imply that MMP-7 is a major MMP associated with the tissue remodeling during the progression of liver fibrosis in biliary atresia.
- We assessed mRNA expression of MMPs in six human colorectal cancer cell lines and found a considerable level of MMP-7 expression in HT-29 cells.
- results suggest E1A-F is overexpressed in early stages of human CRC development and may be an important factor in the overexpression of COX-2 and MMP-7
- Activation of MMP-7 protein closely involved in disruption of tight junction structure and consequent induction of cell dissociation as well as invasion in pancreatic cancer
- Some green tea catechins induce pro-MMP-7 production via O2- production and the activation of JNK1/2.
- expression up-regulated in Helicobacter species-infected colon and bile duct epithelial cells and hepatocytes.
- Polymorphism might be a candidate marker for predicting individuals who are at higher risk to certain tumors but might not be used to predict potential of lymphatic metastasis in various cancers.
- sFasL and matrilysin are expressed in seminal plasma and have a role in regulation of the function of the Fas system
- MMP7 immunoreactivity was present in only polymyositis not dermatomyositis
- Study suggests that targeting matrilysin may have important therapeutic implications.
- Increased expression of MMP-7 in high grade uterine endometrial carcinoma (UEC) may be associated with tumor invasion and metastasis, and MMP-7 could serve as a prognostic maker in UEC.
- shed syndecan-1 or MMP7-syndecan-1 complexes may have roles in tumor progression
- Fas downregulation and a consequential increased resistance to FasL-triggered apoptosis resulting from upregulated MMP-7 in colorectal cancer cells could be a key mechanism for their escape from the immune surveillance
- Data suggest that tissue expression of MMP-7 could be a useful marker to predict the progression and metastasis of hepatocellular carcinoma.
- it is likely that binding of matrilysin to cholesterol sulfate facilitates the matrilysin-catalyzed modulation of cell surface proteins, thus inducing the cancer cell aggregation
- FGF-2-induced MMP7 expression in endothelium depends on AP-1 and FGF-2 signaling to AP-1 involves a Stat1/3-dependent pathway.
- intense MMP-7 signal in tumor cells is an independent prognostic factor predicting better survival in epithelial ovarian cancer
- The present result, for the first time, suggested that the MMP-7-181A/G polymorphism might be associated with the susceptibility to adult astrocytoma.
- Matrix metalloproteinase-7 overexpression is thought to be an early event in the colonic adenoma-carcinoma pathway.
- These data demonstrate that CD151 is overexpressed in osteoarthritic cartilage and suggest that CD151 plays a role in the pericellular activation of proMMP-7, leading to cartilage destruction and/or chondrocyte cloning.
- Data show that MMP7 may be relevant with carcinogenesis, development and metastasis of adenoid cystic carcinoma, and different metastasis potential may result from different subtype of MMPs gene family.
- Estrogen modulates tight junctional resistance through estrogen receptor-alpha-mediated remodeling of occludin
- MMP-7 secretion/activation may represent a new mechanism that facilitates ovarian cancer invasion.
- A significant increase in the proportion of MMP 7 immunopositive cells was seen in the nucleus pulposus of discs classified as showing intermediate levels of degeneration and a further increase was seen in discs with severe degeneration.
- MMP-7 influences tumor progression by regulating invasion and angiogenesis. Multivariate analysis showed that MMP-7 status of cancer tissues was strong predictor of poor prognosis. MMP-7 targeting treatment may be a potential target against human RCC.
- Data show that MMP7 and LN5 are coexpressed in colonic carcinoma cells HT29 cells.
- Our results suggested the importance of MMP-7 and MT1-MMP in the peritoneal metastases of gastric cancer.
- MMP-7 is associated with the innate host defense in periodontal tissues.
- MMP-7 has a role in invasiveness of glioma
- MMP-7-selective targeting to the plasma membrane of epithelial cells promotes its activity by conferring resistance to TIMP-2.
- incubation with HGF mediated the release of MMP-7, resulting in extracellular cleavage of E-cadherin from stomach cancer cells
- MMP-7 is an independent prognostic factor for survival in advanced colorectal cancer. In our sample, the risk of death associated to MMP-7 increase is much higher than the risk of death associated to lactate dehydrogenase elevation
- reveal the MMP7-activated photodynamic therapy efficacy of this photodynamic molecular beacons
- The expression of MMP7 was specifically enhanced at the bronchiolo-alveolar duct junction and suggests that MMP7 plays a key role during the bronchioalveolar remodeling.
- Diminished expression of MMP-7 may play a role in fibronectin accumulation in the diabetic kidney in response to advanced glycation end products and/or TGF-beta.
- Expression of MMP7 was evaluated in hepatocellular carcinoma and surrounding non-tumor tissue.
- VEGF, MMP2, 7 and 9, and COX-2 expression is related to progression of advanced uterine cervical cancer in young women
- MMP-7 gene expression is associated with increased risk only for early stages of oral cancer.
- Wnt1 may play a critical role in regulating the intratumoral MMP-7 expression
- MMP-7 is involved in tumor angiogenesis, thereby contributing to malignant growth and hence associated with decreased survival
- evidence that matrix metalloproteinase-7 (MMP-7) interacts with anionic, cationic and neutral lipid membranes, although it interacts strongest with anionic membranes.
- the significantly increased expression of MMP7 in primary CNS lymphoma (PCNSL), suggest the involvement of this MMP in the characteristic infiltration pattern of PCNSL.
- Circulating Pro-MMP-7 is associated with IgG specific to pro-MMP-7 in patients with renal cell carcinomas
- Immunoassays showed significantly elevated content of matrix metalloproteinase 7 in tumors compared to adjacent histologically unchanged mucosa of patients with colorectal cancer.
- Expression of MMP-7 in cholangiocarcinoma is an unfavorable postoperative prognostic factor of cholangiocarcinoma arising from the large bile ducts. Underexpression of MMPs in papillary cholangiocarcinoma might be associated with a favorable prognosis.
- matrilysin may be multiple, multifarious, and multifaceted functions contributing to early tumor growth
- reduced extracellular proteolytic activity of certain matrix metalloproteinases-for example, MMP-7-is associated with accelerated aging and senescence in normal HMECs.
- TIMP-1 interacts with matrix metalloproteinases and regulates matrilysin activity during airway epithelial repair.
- HER2-dependent transcriptional upregulation and protein secretion of MMP-7 are mediated by activated STAT3.
- Increased plasma MMP-7 is associated with metastatic disease in patients with renal cell carcinomas
- MMP7 and MMP1, are overexpressed in the lung microenvironment and distinguish idiopathic pulmonary fibrosis from other chronic lung diseases. Additionally, increased MMP7 concentration may be indicative of asymptomatic ILD and reflect disease progression
- Individuals with MMP-7 -181GG genotype are at significantly higher risk of cervical cancer.
- HLE may be a natural inactivator of MMP-7 which can counteract MMP-7-induced apoptosis resistance.
- MMP7 may contribute to the process by which intraductal papillary mucinous neoplasm (IPMN) of the pancreas advances from adenoma to carcinoma and to subsequent invasion of tumor cells in IPMN.
- E1AF mRNA overexpression correlated well with matrilysin expression in rectal cancers.
- MMP7 may act as a downstream mediator of FVIIa/TF signal transduction to facilitate the development of metastasis in colon cancer.
- HRG-beta-induced MMP-7 expression was regulated by HER2-mediated AP-1 activation in MCF-7 cells.
- Higher matrix metallopeptidase 7 is associated with colorectal cancer.
- MMP-7 cleaves the NR1 subunit of the N-methyl-d-aspartate (NMDA) receptor to generate an N-terminal fragment of approximately 65 kDa
- Results from our study suggest that common MMP-7 genetic polymorphisms may contribute to breast cancer susceptibility
- p120 and Kaiso regulate Helicobacter pylori-induced expression of matrix metalloproteinase-7
- beta-catenin expression was significantly related to E-cadherin and MMP-7 expression
- activin A may modulate the expression of MMP-7 via AP-1
- MMP7 and MMP9-mediated cleavage of EphB2 is induced by receptor-ligand interactions at the cell surface and that this event triggers cell-repulsive responses
- The matrix metalloproteinase-7 gene may be up-regulated by mutated or continuously elevated beta-catenin protein in sporadic desmoid tumors.
- It is supposed that the cleavage of cell surface annexin II by matrilysin contributes to tumor invasion and metastasis by enhancing tPA-mediated pericellular proteolysis by cancer cells.
- Functional polymorphisms in the promoter of MMP-7 do not significantly confer susceptibility to hepatocellular carcinoma in a southern Chinese population.
- common MMP-7 genetic polymorphisms may have roles in survival among Chinese women with breast cancer
- Gene expression revealed up-regulation of pro-angiogenic (PGF), anti-apoptotics (BAG-1, BCL-2), heart development (TNNT2, TNNC1) and extracellular matrix remodelling (MMP-2, MMP-7) genes in SM.
- the expression level of MMP-7 in tumor cells is predictive of response to chemotherapy and outcome in patients with advanced NSCLC receiving platinum-based chemotherapy.
- metalloproteinase-7 expression was associated with more aggressive buccal squamous cell carcinomas
- Identification of amino acid residues of matrix metalloproteinase-7 essential for binding to cholesterol sulfate.
- In Japan, carriage of the MMP-7-181 G allele and of the CMA/B A allele were each associated with an increased risk for H. pylori-related noncardia gastric cancer development.
- MMP7 gene expression was significantly higher in colorectal cancer patients than in healthy volunteers.
- Serous and mucinous ovarian tumors express different profiles of MMP-7.
- Hyaluronan synthase (HAS2 and HAS3) and hyaluronan may mediate cellular invasion via changes in MMP-7 expression in SW620 colon carcinoma cells.